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AB762

Anti-CD34 抗体 [4H11(APG)]

Anti-CD34 antibody [4H11[APG]]

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(10 Publications)

Mouse Monoclonal CD34 antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Cell preparation containing CD34 protein.

別名を表示する

CD34, Hematopoietic progenitor cell antigen CD34

2 Images
Flow Cytometry - Anti-CD34 antibody [4H11[APG]] (AB762)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD34 antibody [4H11[APG]] (AB762)

Flow cytometric surface staining pattern showing CD34 positive stem cells in red stained with 2μg/ml ab762 in human peripheral whole blood.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [4H11[APG]] (AB762)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [4H11[APG]] (AB762)

IHC image of CD34 staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab762, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

関連する標識済み抗体及び組成の異なる製品 (5)

  • 723 Alexa Fluor® 700

    Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]]

  • Biotin

    Biotin Anti-CD34 antibody [4H11[APG]]

  • 519 FITC

    FITC Anti-CD34 antibody [4H11[APG]]

  • 578 PE

    PE Anti-CD34 antibody [4H11[APG]]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD34 antibody 4H11[APG]

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

4H11[APG]

アイソタイプ

IgG1

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt, IHC-P

applications

免疫原

Cell preparation containing CD34 protein. The exact immunogen used to generate this antibody is proprietary information.

P28906

特異性

This antibody reacts with Class III epitope on CD34 antigen.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
精製に関する特記事項
Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE.
バッファー組成
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
See full target information CD34
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文献 (10)

Recent publications for all applications. Explore the full list and refine your search

International journal of immunopathology and pharmacology 39:3946320251315007 PubMed39921228

2025

INF-γ/TGF-β1-primed umbilical cord mesenchymal stem cells boost the T-lymphocytes activity: Modulation of CD25 expression and IL-6 secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Abdulrahman H Almaeen,Heba M Saad-Eldien,Hala Gabr

International journal of molecular sciences 24: PubMed37108645

2023

Vascular Stem Cells and the Role of B-Raf Kinase in Survival, Proliferation, and Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dipali Goyal,Sean W Limesand,Ravi Goyal

BMC pulmonary medicine 22:377 PubMed36207695

2022

Relationship between the expression of ARHGAP25 and RhoA in non-small cell lung cancer and vasculogenic mimicry.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Shi,Jiatao Wu,Qianhao Jia,Kairui Li,Wenjuan Li,Yuqi Shi,Yufei Wang,Shiwu Wu

Cancer management and research 13:5919-5930 PubMed34354374

2021

The Relationship Between UBE2C and AGGF1 Overexpression and Tumor Angiogenesis in Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yufei Wang,Fan Shi,Run Tao,Jiatao Wu,Jinxiang Gu,Ruixue Yang,Shiwu Wu

Medicine 100:e24472 PubMed33530259

2021

Low PRRX1 expression and high ZEB1 expression are significantly correlated with epithelial-mesenchymal transition and tumor angiogenesis in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ruixue Yang,Yuanqun Liu,Yufei Wang,Xiaolin Wang,Hongfei Ci,Chao Song,Shiwu Wu

Diagnostic pathology 15:14 PubMed32035486

2020

FOXM 1 induces Vasculogenic mimicry in esophageal cancer through β-catenin /Tcf4 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Cheng,Qi Wang,Xiaoying Tao,Yanzi Qin,Qiong Wu,Dafang Zheng,Damin Chai,Yong Zhang,Dongbing Lu,Hongfei Ci,Zhiwei Wang,Jia Ma,Danna Wang,Zenong Cheng,Shiwu Wu,Yisheng Tao

Cancer control : journal of the Moffitt Cancer Center 26:1073274819875736 PubMed31533461

2019

Aberrant Cyclin E and Hepatocyte Growth Factor Expression, Microvascular Density, and Micro-Lymphatic Vessel Density in Esophageal Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Xie,Yuanyuan Wang,Jingkang He,Zhengzheng Ni,Damin Chai

International journal of clinical and experimental pathology 12:2205-2214 PubMed31934043

2019

Aberrant expression of MYH9 and E-cadherin in esophageal squamous cell carcinoma and their relationship to vasculogenic mimicry.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Cheng,Xiaoying Tao,Yanzi Qin,Jingping Wang,Jing Xu,Hongfei Ci,Qiong Wu,Dafang Zheng,Qi Wang,Zenong Cheng,Shiwu Wu,Yisheng Tao

Bioengineering (Basel, Switzerland) 6: PubMed31159244

2019

A Microcavity Array-Based 4D Cell Culture Platform.

Applications

Unspecified application

Species

Unspecified reactive species

Cordula Nies,Tobias Rubner,Hanna Lorig,Vera Colditz,Helen Seelmann,Andreas Müller,Eric Gottwald

The Journal of biological chemistry 288:13325-36 PubMed23519467

2013

Dual mechanism of integrin αIIbβ3 closure in procoagulant platelets.

Applications

Unspecified application

Species

Unspecified reactive species

Nadine J A Mattheij,Karen Gilio,Roger van Kruchten,Shawn M Jobe,Adam J Wieschhaus,Athar H Chishti,Peter Collins,Johan W M Heemskerk,Judith M E M Cosemans
View all publications
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代替製品

Primary Antibodies

AB195013

Alexa Fluor® 488 Anti-CD34 antibody [EP373Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD34 antibody [EP373Y] (AB195013)

  • 5
  • 5
Primary Antibodies

AB195017

HRP Anti-CD34 antibody [EP373Y]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CD34 antibody [EP373Y] (AB195017)

  • 0
  • 0
Primary Antibodies

AB223930

PE Anti-CD34 antibody [EP373Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-CD34 antibody [EP373Y] (AB223930)

  • 5
  • 1
Primary Antibodies

AB81289

Anti-CD34 antibody [EP373Y]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-CD34 antibody [EP373Y] (AB81289)

  • 4
  • 56
Primary Antibodies

AB198395

Anti-CD34 antibody [EP373Y] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (AB198395)

  • 4
  • 4

組合せ製品

Assay Kits

AB323933

Human CD34 ELISA Kit- Intracellular

SimpleStep|Recombinant

Sandwich ELISA - Human CD34 ELISA Kit- Intracellular (AB323933)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB10558

Anti-CD45 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (AB10558)

  • 4
  • 62
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB28364

Anti-CD31 antibody

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB28364)

  • 4
  • 161

Abcam product promise

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