Anti-CD33 抗体 [WM53] (ab30371)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [WM53] to CD33
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD33 antibody [WM53]
CD33 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [WM53] to CD33 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human CD33. Human AML cells.
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ポジティブ・コントロール
- ICC/IF: HL-60 cells Flow Cyt: Human whole blood
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特記事項
This product has switched from a hybridoma to recombinant production method on 21st September 2020.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
WM53 -
ミエローマ
NS1 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
- APC Anti-CD33 antibody [WM53] (ab118967)
- PerCP Anti-CD33 antibody [WM53] (ab124531)
- APC/Cy7® Anti-CD33 antibody [WM53] (ab206634)
- PE/Cy5® Anti-CD33 antibody [WM53] (ab233448)
- PE Anti-CD33 antibody [WM53] (ab233577)
- FITC Anti-CD33 antibody [WM53] (ab234216)
- PE/Cy7® Anti-CD33 antibody [WM53] (ab239310)
- Anti-CD33 antibody [WM53] - BSA and Azide free (ab252263)
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab30371の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC/IF |
Use a concentration of 10 µg/ml.
Works in both 4% PFA fixed cells (10mins) and 100% MeOH fixed cells (5mins) |
特記事項 |
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Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use a concentration of 10 µg/ml. Works in both 4% PFA fixed cells (10mins) and 100% MeOH fixed cells (5mins) |
ターゲット情報
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機能
Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). -
組織特異性
Monocytic/myeloid lineage cells. -
配列類似性
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
ドメイン
Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
翻訳後修飾
Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 945 Human
- Omim: 159590 Human
- SwissProt: P20138 Human
- Unigene: 83731 Human
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別名
- CD 33 antibody
- CD33 antibody
- CD33 antigen (gp67) antibody
see all
画像
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ab30371 staining CD33 in HL-60 cells. The cells were fixed with 4% paraformaldehyde (10min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab30371 at 10μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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Flow cytometry staining of human whole blood with ab30371 (right) or mouse IgG1 and kappa; (ab170190) isotype (left). Red blood cells of 200 µL were lysed, then cells were incubated for 30 mins on ice in 1 x PBS containing 10 µg/mL human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interactions followed by antibody (ab30371) or mouse IgG1 & kappa; (ab170190) isotype for 30 min on ice. The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor 488, Pre-absorbed) (ab150119) was used at 1/2000 dilution for 30 min on ice. The cells were simultaneously stained with CD14. Ac
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab30371 は 2 報の論文で使用されています。
- Wißfeld J et al. Deletion of Alzheimer's disease-associated CD33 results in an inflammatory human microglia phenotype. Glia 69:1393-1412 (2021). PubMed: 33539598
- Ornatsky O et al. Highly multiparametric analysis by mass cytometry. J Immunol Methods 361:1-20 (2010). Flow Cyt ; Human . PubMed: 20655312