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リコンビナントRabMAb

Anti-CD32B (phospho Y292) 抗体 [EP926Y] (ab68423)

Submit a review Q&A (3)References (4)
Immunoprecipitation - Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)
  • Western blot - Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)
  • Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP926Y] to CD32B (phospho Y292)
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-CD32B (phospho Y292) antibody [EP926Y]
    CD32B 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP926Y] to CD32B (phospho Y292)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC/IF or IHC-P

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IP: Raji whole cell lysate WB: Daudi (Human Burkitt's lymphoma lymphoblast) treated with 10mM pervanadate for 30 minutes whole cell lysate

  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab68423の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/50000 - 1/100000. Detects a band of approximately 40,44 kDa (predicted molecular weight: 40,44 kDa).
IP
1/20.
特記事項
WB
1/50000 - 1/100000. Detects a band of approximately 40,44 kDa (predicted molecular weight: 40,44 kDa).
IP
1/20.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

ターゲット情報

  • 機能

    Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.

  • 組織特異性

    Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.

  • 関連疾患

    Note=A chromosomal aberration involving FCGR2B is found in a follicular lymphoma. Translocation t(1;22)(q22;q11). The translocation leads to the hyperexpression of the receptor. This may play a role in the tumor progression.

  • 配列類似性

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

  • ドメイン

    Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD32 antibody
      • CD32 antigen antibody
      • CD32B antibody
      • CDw32 antibody
      • Fc fragment of IgG, low affinity II, receptor for (CD32) antibody
      • Fc fragment of IgG, low affinity IIb, receptor antibody
      • Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody
      • Fc fragment of IgG, low affinity IIb, receptor for (CD32) antibody
      • Fc fragment of IgG, low affinity IIb, receptor for antibody
      • Fc gamma receptor IIB antibody
      • Fc gamma RII antibody
      • Fc gamma RIIB antibody
      • Fc-gamma RII antibody
      • Fc-gamma RII-b antibody
      • Fc-gamma-RIIb antibody
      • FCG2 antibody
      • FCG2B_HUMAN antibody
      • Fcgr2 antibody
      • FCGR2B antibody
      • FCRII antibody
      • FcRII-b antibody
      • IGFR2 antibody
      • IgG Fc receptor II beta antibody
      • IgG Fc receptor II-b antibody
      • Low affinity immunoglobulin gamma Fc region receptor II antibody
      • Low affinity immunoglobulin gamma Fc region receptor II-b antibody
      • Ly-17 antibody
      • Lym 1 antibody
      • Lymphocyte antigen 17 antibody
      see all

    画像

    • Immunoprecipitation - Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)
      Immunoprecipitation - Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)

      ab68423 at 1/20 dilution immunoprecipitating CD32B  in Raji (Human Burkitt's lymphoma) whole cell lysates.
      Lane 1 (input): Raji (Human Burkitt's lymphoma) treated with 10mM pervanadate for 30 minutes whole cell lysate 10μg
      Lane 2 (+): Raji treated with 10mM pervanadate for 30 minutes whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68423 in Raji treated with 10mM pervanadate for 30 minutes whole cell lysate
      For western blotting, ab68423  at 1/20 dilution and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.

      Expsoure time: 1 second

      Observed MW: 40-44 KDa

    • Western blot - Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)
      Western blot - Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)
      All lanes : Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423) at 1/100000 dilution

      Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
      Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) treated with 10mM pervanadate for 30 minutes whole cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 40,44 kDa



      Exposure time: 5 seconds

      Observed MW: 40-44 KDa

      Blocking/diluting buffer and concentration: 5% NFDM/TBST

    • Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)
      Anti-CD32B (phospho Y292) antibody [EP926Y] (ab68423)

    参考文献 (4)

    ab68423 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab68423 は 4 報の論文で使用されています。

    • Beneduce C  et al. Inhibitory Fc-Gamma IIb Receptor Signaling Induced by Multivalent IgG-Fc Is Dependent on Sialylation. Cells 12:N/A (2023). PubMed: 37681862
    • Rieth N  et al. Characterization of SM201, an anti-hFc?RIIB antibody not interfering with ligand binding that mediates immune complex dependent inhibition of B cells. Immunol Lett 160:145-50 (2014). PubMed: 24472604
    • Sundgren NC  et al. Coupling of fc? receptor I to fc? receptor IIb by SRC kinase mediates C-reactive protein impairment of endothelial function. Circ Res 109:1132-40 (2011). PubMed: 21940940
    • Horton HM  et al. Antibody-mediated coengagement of Fc?RIIb and B cell receptor complex suppresses humoral immunity in systemic lupus erythematosus. J Immunol 186:4223-33 (2011). PubMed: 21357255

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Question

    Sind die AKs specifisch für die verschiedenen Isotypen IIa oder b oder c?

    Read More
    Answer

    Vielen Dank für Ihren Anruf.

    Ich kann Ihnen nun bestätigen, dass beide Antikörper spezifisch die IIb Isoform (und alle Unterformen von dieser Isoform: IIb1, IIb2, IIb3) binden.

    Beide Immunogen sind Peptide die die folgende Sequenz einschließt: TITYpSLL.

    Die Antikörper wurden nicht experimentell mit Isoform IIa und IIc getestet. Aber diese beiden Isoformen enthalten die Immunogensequenz TITYpSLL nicht.

    Ich hoffe, diese Information ist hilfreich und wünsche Ihnen viel Erfolg mit Ihrer Forschung.

    Read More

    Question

    Does ab68423 detect CD32A, CD32B, or both?

    Read More
    Answer

    ab68423 detects CD32b and is not expected to cross-react with CD32a. However, this has not been specifically tested in the laboratory as far as I am aware.

    Read More

    Question

    Inquiry: I noticed that the Epitomics data sheet for this antibody (clone EP926Y) states that it cross reacts with mouse CD32b (phospho Y292), as determined by western blot. Abcam states that it does not cross react with mouse or rat. I'm wondering which is true, or why there is this descrepancy. This is in reference to Abcam # ab68423 or Epitomics # 2308-1. Thanks.

    Read More
    Answer

    Thank you very much for contacting us with your question.

    I apologize for the confusing discrepancy on the Abcam and Epitomics datasheets. I've gotten in touch with the Epitomics lab, and they did test the antibody once on mouse samples (brain, heart, kidney and spleen) at a 1:100,000 primary dilution but did not observe a signal. They still recommend the product for mouse reactivity based on the fact that the immunogen sequence has 100% homology with the mouse protein.

    Though Epitomics is now an Abcam company, we are still working to merge all of our policies. Abcam only lists a species as reactive if it has actually been tested and shown positive results. If the results were negative then we say the antibody does not react with that species.

    Due to the above information, I hesitate to recommend this antibody for use with mouse samples. There may be tweaks to the protocol that would produce a signal with mouse lysates (such as increasing the concentration, 1:100,000 is quite high) but we can not guarantee this antibody to work with mouse.

    I hope that this information will be useful, but please let me know if you have any further questions and I'll be happy to help. Have a nice day.

    Read More

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