JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB180175

Anti-CD31 (phospho Y713) 抗体 [EPR8079(2)] - C-terminal

Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Recombinant Monoclonal CD31 phospho Y713 antibody. C-terminal. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 3 publications.

別名を表示する

CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1

2 Images
Western blot - Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (AB180175)
  • WB

Supplier Data

Western blot - Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (AB180175)

All lanes:

Western blot - Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (ab180175) at 1/1000 dilution

Lane 1:

Non-treated Jurkat cell lysate at 10 µg

Lane 2:

Pervanadate-treated Jurkat cell lysate at 10 µg

Secondary

All lanes:

Standard HRP goat anti-rabbit at 1/2000 dilution

Predicted band size: 82 kDa

true

Dot Blot - Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (AB180175)
  • Dot

Lab

Dot Blot - Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (AB180175)

Dot blot analysis of CD31 (pY713) peptide (Lane 1) and CD31 non-phospho peptide (Lane 2) labelling CD31 (phospho Y713) with ab180175 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 10 seconds.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR8079(2)

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, Dot

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary.</p><p>The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31.</p><p>Hu Isoform 1-6: 79-83 kDa (predicted).</p><p>Observed band size is around 130 kDa.</p><p>Positive Control: HUVEC and Jurkat cell lysates (<a href='/products/cell-lysates/jurkat-whole-cell-lysate-ab7899'>ab7899</a>); Human spleen and kidney tissue lysate.</p><p>Negative Control: NIH/3T3 whole cell lysate (<a href='/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>).</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

出荷温度及び保存条件

製品の状態
Liquid
精製度
Tissue culture supernatant
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed : 17580308, PubMed : 19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed : 19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed : 27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed : 17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed : 12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed : 12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed : 18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed : 18672896). Induces susceptibility to atherosclerosis (By similarity).. Isoform Delta15. Does not protect against apoptosis.
See full target information CD31 phospho Y713

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed37549051

2023

CD31 signaling promotes the detachment at the uropod of extravasating neutrophils allowing their migration to sites of inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco Andreata,Marc Clément,Robert A Benson,Juliette Hadchouel,Emanuele Procopio,Guillaume Even,Julie Vorbe,Samira Benadda,Véronique Ollivier,Benoit Ho-Tin-Noe,Marie Le Borgne,Pasquale Maffia,Antonino Nicoletti,Giuseppina Caligiuri

Cancer cell international 20:1 PubMed31908598

2020

Patient-derived xenografts of different grade gliomas retain the heterogeneous histological and genetic features of human gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Wenxin Zeng,Zhaohua Tang,Yongguo Li,Guangnian Yin,Zili Liu,Jie Gao,Yan Chen,Feilan Chen

Oncology letters 9:1089-1094 PubMed25663861

2015

Cross-talk between endothelial and tumor cells via basic fibroblast growth factor and vascular endothelial growth factor signaling promotes lung cancer growth and angiogenesis.

Applications

IF

Species

Unspecified reactive species

Heng DU,Hui Shi,Dali Chen,Yubin Zhou,Guowei Che
View all publications

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com