Anti-CD31 抗体 [TLD-3A12] (ab64543)
![Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (ab64543)](https://www.abcam.co.jp/ps/products/64/ab64543/Images/ab64543-77917-anti-cd31-antibody-tld-3a12-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (ab64543)")
Key features and details
- Mouse monoclonal [TLD-3A12] to CD31
- Suitable for: IHC-Fr
- Reacts with: Rat
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-CD31 antibody [TLD-3A12]
CD31 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [TLD-3A12] to CD31 -
由来種
Mouse -
特異性
ab64543 recognises rat PECAM-1 (CD31). It also partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. -
アプリケーション
適用あり: IHC-Frmore details
適用なし: IHC-P -
種交差性
交差種: Rat
交差が予測される動物種: Pig, Rhesus monkey
非交差種:
Human -
免疫原
Tissue, cells or virus corresponding to Rat CD31. Activated, Lewis rat derived microglial cells
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ポジティブ･コントロール
- Rat spleen; activated microglial cells.
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特記事項
This antibody has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
This antibody has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. -
ポリ/モノ
モノクローナル -
クローン名
TLD-3A12 -
ミエローマ
Sp2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64543の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | (3) |
1/10 - 1/100.
A fixation with acetone or methanol is recommended. Do not fix with PFA. |
| 特記事項 |
|---|
|
IHC-Fr
1/10 - 1/100. A fixation with acetone or methanol is recommended. Do not fix with PFA. |
追加情報
Is unsuitable for IHC-P.
ターゲット情報
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機能
Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC). -
組織特異性
Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level). -
配列類似性
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
ドメイン
The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity. -
翻訳後修飾
Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. -
細胞内局在
Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells. - Information by UniProt
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参照データベース
- Entrez Gene: 29583 Rat
- SwissProt: Q3SWT0 Rat
- Unigene: 1878 Rat
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別名
- Adhesion molecule antibody
- CD31 antibody
- CD31 antigen antibody
see all
画像
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Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (ab64543)Immunohistochemical staining of rat brain cryosection with Mouse anti Rat CD31 (ab64543)
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![Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (ab64543)](https://www.abcam.co.jp/ps/products/64/ab64543/Images/ab64543-77918-anti-cd31-antibody-tld-3a12-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (ab64543)This image is courtesy of Valley Lasry.ab64543 staining CD31 in rat brain tissue by Immunohistochemistry (Frozen sections). The sections were fixed in paraformaldehyde prior to blocking with 5% donkey serum, BSA 1%, Triton 1.5% for 2 hours at room temperature. The primary antibody was diluted 1/200 in PBS/5% Donkey serum and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 555-conjugated donkey anti-mouse polyclonal was used as the secondary antibody, diluted 1/200. -
![Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (ab64543)](https://www.abcam.co.jp/ps/products/64/ab64543/Images/ab64543-77916-anti-cd31-antibody-tld-3a12-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (ab64543)Immunohistochemical staining of rat spleen cryosection with Mouse anti Rat CD31 (ab64543)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (79)
ab64543 は 79 報の論文で使用されています。
- Li S et al. Enhanced Bone Regeneration through Regulation of Mechanoresponsive FAK-ERK1/2 Signaling by ZINC40099027 during Distraction Osteogenesis. Int J Med Sci 21:137-150 (2024). PubMed: 38164350
- Fan Z et al. Simulation of Cortical and Cancellous Bone to Accelerate Tissue Regeneration. Adv Funct Mater 33:N/A (2023). PubMed: 37601745
- Polo-Montalvo A et al. Effects of graphene oxide and reduced graphene oxide nanomaterials on porcine endothelial progenitor cells. Nanoscale 15:17173-17183 (2023). PubMed: 37853851
- Liu C et al. Overexpression of vascular endothelial growth factor enhances the neuroprotective effects of bone marrow mesenchymal stem cell transplantation in ischemic stroke. Neural Regen Res 18:1286-1292 (2023). PubMed: 36453413
- Wei D et al. Edgeworthia gardneri (Wall.) Meisn. extract protects against myocardial infarction by inhibiting NF-κB-and MAPK-mediated endothelial inflammation. Front Cardiovasc Med 9:1013013 (2022). PubMed: 36606274