Name: Anti-CD31 antibody [RM0032-1D12] - BSA and Azide free
SKU: ab56299
Rating: 4 (7 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] - BSA and Azide free (ab56299)

Key features and details

Rat monoclonal [RM0032-1D12] to CD31 - BSA and Azide free
Suitable for: IHC-P
Reacts with: Mouse
Isotype: IgG2a

リコンビナント抗体で、ロット間での高い再現性を実現

Anti-CD31 antibody [EPR17259] (ab182981)

異なるロット間での安定した再現性
容易なスケールアップ
評価試験による特異性の確認済み
倫理基準に準拠 - アニマル・フリーの生産

製品名

Anti-CD31 antibody [RM0032-1D12] - BSA and Azide free
CD31 一次抗体製品一覧
製品の詳細

Rat monoclonal [RM0032-1D12] to CD31 - BSA and Azide free
由来種

Rat
アプリケーション

適用あり: IHC-Pmore details
種交差性

交差種: Mouse
免疫原

Full length protein corresponding to Mouse CD31. Endothelial membrane protein
ポジティブ･コントロール
- IHC-P: Mouse kidney, lung, heart and liver tissue.
特記事項

Proteinase K antigen retrieval is essential.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の状態

Liquid
保存方法

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファー

Constituent: PBS
キャリア・フリー

はい
Concentration information loading...
精製度

Protein G purified
特記事項（精製）

Purified from tissue culture supernatant and lyophilized from a 0.2 µm filtered solution in phosphate-buffered saline (PBS).
ポリ/モノ

モノクローナル
クローン名

RM0032-1D12
アイソタイプ

IgG2a
研究分野

Compatible Secondaries
- Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
- Goat Anti-Rat IgG H&L (HRP) (ab205720)
Isotype control
- Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
Recombinant Protein
- Recombinant human CD31 protein (ab155607)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab56299の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
IHC-P	(5)	1/50 - 1/400. 4% PFA ix preferred fixative over formalin. Fixation should be overnight. Carrying out Proteinase K enzymatic retrieval is essential (please see specific protocol below and Abreviews). A sensitive detection system such as HRP polymer system is recommended. The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue

特記事項

IHC-P
1/50 - 1/400.

4% PFA ix preferred fixative over formalin. Fixation should be overnight. Carrying out Proteinase K enzymatic retrieval is essential (please see specific protocol below and Abreviews). A sensitive detection system such as HRP polymer system is recommended.

The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples.

Positive Control: Hu tonsil tissue

機能

Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
組織特異性

Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
配列類似性

Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
ドメイン

The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
翻訳後修飾

Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
細胞内局在

Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
Target information above from: UniProt accession P16284 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 18613 Mouse
- SwissProt: Q08481 Mouse
- Unigene: 343951 Mouse
別名
- Adhesion molecule antibody
- CD31 antibody
- CD31 antigen antibody
- CD31 EndoCAM antibody
- EndoCAM antibody
- FLJ34100 antibody
- FLJ58394 antibody
- GPIIA antibody
- GPIIA' antibody
- PECA1 antibody
- PECA1_HUMAN antibody
- Pecam 1 antibody
- PECAM 1 CD31 EndoCAM antibody
- PECAM antibody
- PECAM-1 antibody
- Pecam1 antibody
- Platelet and endothelial cell adhesion molecule 1 antibody
- Platelet endothelial cell adhesion molecule antibody
- Platelet/endothelial cell adhesion molecule 1 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] - BSA and Azide free (ab56299)Yoshida et al PLoS One. 2016 Aug 2;11(8):e0160330. doi: 10.1371/journal.pone.0160330. eCollection 2016. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Tumor vascularization and VEGF in ascites.

(A) ID8, ID8-c-MYC, or ID8-KRAS cells (2 × 10⁶) were intra-peritoneal injected into C57/BL6 mice. Mice were sacrificed when the body weight reached 23 g. Disseminations were obtained from mice when sacrificed. Tumor vascularization of each dissemination was assessed using CD31 immunohistochemistry (ab56299).

Scale bars represent 200 μm and 50 μm at low and high magnification, respectively.

Paraffin sections (4-μm-thick) of the biggest tumor sections from ID8, ID8-c-MYC, and ID8-KRAS mice were dewaxed in xylene and rehydrated through graded ethanol to water. Antigens were retrieved by boiling in 10 mM citrate buffer (pH 6.0) for 30 min. The cooled sections were incubated in a peroxidase blocking solution for 10 min to quench endogenous peroxidase. Sections were incubated in a protein blocking solution at room temperature for 10 min to block non-specific binding. Sections were then stained for Ki67 using rabbit monoclonal antibody against mouse Ki67, CD31 using a rat monoclonal antibody against mouse CD31 (ab56299, Abcam 1:100 dilution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] - BSA and Azide free (ab56299)Vasilopoulou et al PLoS One. 2013 Jun 12;8(6):e65402. doi: 10.1371/journal.pone.0065402. Print 2013. Fig 8. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Proliferation and cell number in male wild-type (+/y) and male Mct8 knockout (−/y) placentae.

(Panel C): Representative picture demonstrating CD31 immunoreactivity (marker of endothelial cells represented by brown staining) in the mouse placenta at E18.5. The nuclei were counterstained using hematoxylin. Endothelial cells are indicated by arrows and trophoblast cells by arrowheads.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] - BSA and Azide free (ab56299)

ab56299 at 1/400 staining CD31 in LPS treated mouse kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] - BSA and Azide free (ab56299)

ab56299 at 1/400 dilution, staining CD31 in LPS treated mouse liver tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] - BSA and Azide free (ab56299)

ab56299 at 1/400 staining CD31 in LPS treated mouse lung tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] - BSA and Azide free (ab56299)

ab56299 at 1/400 dilution, staining CD31 in LPS treated mouse heart tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

Recommended IHC-P protocol with ab56299

Click here to view the general protocols

Datasheet download

Download

ab56299 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab56299 は 81 報の論文で使用されています。

Zou M et al. Prdm6 drives ductus arteriosus closure by promoting ductus arteriosus smooth muscle cell identity and contractility. JCI Insight 8:N/A (2023). PubMed: 36749647
Moghassemi S et al. Ex vivo purging of cancer cells from ovarian tissue using photodynamic therapy: a novel strategy to restore fertility in leukemia patients. Hum Reprod Open 2023:hoad005 (2023). PubMed: 36895885
Lee SR et al. PGRMC1 Ablation Protects from Energy-Starved Heart Failure by Promoting Fatty Acid/Pyruvate Oxidation. Cells 12:N/A (2023). PubMed: 36899888
Huang L et al. FGL2 deficiency alleviates maternal inflammation-induced blood-brain barrier damage by blocking PI3K/NF-κB mediated endothelial oxidative stress. Front Immunol 14:1157027 (2023). PubMed: 37051251
Del Campo Fonseca A et al. Ultrasound trapping and navigation of microrobots in the mouse brain vasculature. Nat Commun 14:5889 (2023). PubMed: 37735158

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-10 of 11 Abreviews or Q&A

Application

Western blot

Sample

Mouse Tissue lysate - nuclear (liver)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

liver

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 13 2015

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Brain)

Specification

Brain

Fixative

Paraformaldehyde

Antigen retrieval step

Enzymatic - Buffer/Enzyme Used: Proteinase K

Permeabilization

Yes - Triton X

Blocking step

Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Kate Schunke

Verified customer

投稿 Aug 20 2012

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (kidney)

Specification

kidney

Fixative

Paraformaldehyde

Antigen retrieval step

Enzymatic - Buffer/Enzyme Used: proteinase K

Permeabilization

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 06 2012

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Heart Frozen Section)

Specification

Heart Frozen Section

Fixative

Paraformaldehyde

Permeabilization

Yes - Triton X-100

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 26 2011

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Skeletal muscle)

Specification

Skeletal muscle

Fixative

10% Formalin

Antigen retrieval step

Enzymatic - Buffer/Enzyme Used: Proteinase K, 10ug/ml, 15 min RT

Permeabilization

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Josh Meisner

Verified customer

投稿 Apr 08 2010

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Human melanoma tumor recovered from nude mice.)

Specification

Human melanoma tumor recovered from nude mice.

Fixative

Formaldehyde

Antigen retrieval step

Enzymatic - Buffer/Enzyme Used: proteinase K 10ug/ml RT 30min

Permeabilization

Blocking step

DakoCytomation Serum Free General Blocking Cat#X0909 as blocking agent for 30 minute(s) · Concentration: 0% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Hongwei Shao

Verified customer

投稿 Oct 22 2009

Question

I´d like to know what is the specification of the epitope, it is N or C terminus?

Abcam community

Verified customer

Asked on Oct 16 2013

Answer

The antibody was raised against a mouse endothelial membrane preparation, and as it is a monoclonal antibody, it could target any region of the protein. The epitope has not been mapped, unfortunately, so we do not know whether it targets one of the termini or an internal region.

XXXXXX XXXXXX

Abcam Scientific Support

Answered on Oct 16 2013

Question

Do you have optimised protocol for paraffin-embedded sections immunofluorescence with Ab56299?

Abcam community

Verified customer

Asked on Feb 04 2013

Answer

On the bottom of the online datasheet for ab56299 there is a "Recommended IHC-P protocol with ab56299" link under the "Protocols" heading. In summation:

1.Dewax and Rehydration: Place the slides in a rack and sequentially pass the rack through following solutions: Histoclear or Xylene twice, 10 minutes each; and then a graded series of 100%, 90%, 70%, and 50% ethanol, 2 minutes each.
2. Wash the slides with tap water for 5 minutes and once with DI water.
3. Antigen Retrieval: treat the slides with 20µg/ml proteinase K in PBS for 15-30 minutes at room temperature.
4. Wash the slides with tap water for 5 minutes and quench the endogenous peroxidase treated in 3% H2O2 in DI water for 30 minutes
5. Wash the slides with tap water for 5mins and soaking in PBS-Tween20 (0.1%)
6. Block the slides with blocking buffer (2.5-25% BSA in PBS-Tween20) for 10 minutes
7. Add primary antibody (Rat against mouse CD31 1:100) and incubate the antibody for 2 hours at room temperature, or for overnight at 4°C.
8. Wash the slides with rotation in PBS-Tween20 for 3 times, 5 minutes each.
9. Add anti rat secondary antibody and incubate at room temperature following manufacturer instructions.
10. Wash the slides with rotation in PBS-Tween20 for 3 times, 5 minutes each.
11. Add Polymer-HRP labeled anti rabbit (Dako: K4010) and incubate for 30 minutes at room temperature.
12. Wash the slides with rotation in PBS-Tween20 for 3 times, 5 minutes each.
13. Perform DAB Color development (reagents available in Dako K4010).. 15. Wash with tap water and count stain the nuclear with hematoxin.

Abcam Scientific Support

Answered on Feb 04 2013

Question

The customer did try their experiment with a single secondary antibody and only got weak signal. So they would like to try your suggested protocol and were wondering if your secondary rabbit anti-rat ab6703 would be suitable for that purpose.

Abcam community

Verified customer

Asked on Feb 29 2012

Answer

Ab6703 should work as the secondary antibody in the amplification step.

Abcam Scientific Support

Answered on Feb 29 2012

Question

I have an enquiry about the “Recommended IHC-P protocol with ab56299 for Anti-CD31 antibody [RM0032-1D12]”, why do they recommend the use of 2 secondary antibodies (rabbit anti rat followed by HRP-anti-rabbit), instead of just HRP anti-rat.

The second reference (in Abcam website): http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=20145133&dopt=Abstract - there, they use only one secondary antibody i.e. biotinylated goat anti-rat.

The customer would like to know if they can use only one HRP-secondary antibody. They don’t have the 2 recommended secondary antibodies.

Abcam community

Verified customer

Asked on Feb 24 2012

Answer

We recommend a double secondary antibody application to add an amplification step to increase the strength of the signal. You can use a single secondary step, but if they see a weak signal, then they may have to go to the double secondary method to get a stronger signal.

Abcam Scientific Support

Answered on Feb 24 2012

1-10 of 11 Abreviews or Q&A

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Key features and details

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

キャリア・フリー

精製度

特記事項（精製）

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Compatible Secondaries

Isotype control

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

ドメイン

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

Datasheet download

参考文献 (81)

レビューと Q&A

Western blot abreview for Anti-CD31 antibody [RM0032-1D12]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD31 antibody [RM0032-1D12]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD31 antibody [RM0032-1D12]

Immunohistochemistry (Frozen sections) abreview for Anti-CD31 antibody [RM0032-1D12]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD31 antibody [RM0032-1D12]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD31 antibody [RM0032-1D12]