JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB7388

Anti-CD31 抗体 [MEC 7.46]

Anti-CD31 antibody [MEC 7.46]

3

(8 Reviews)

|

(166 Publications)

Anti-CD31 antibody [MEC 7.46] (ab7388) is a rat monoclonal antibody detecting CD31 in Flow Cytometry, ICC/IF. Suitable for Mouse.

- Over 130 publications
- Trusted since 2002

別名を表示する

CD31, Pecam, Pecam-1, Pecam1, Platelet endothelial cell adhesion molecule, PECAM-1

2 Images
Flow Cytometry - Anti-CD31 antibody [MEC 7.46] (AB7388)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD31 antibody [MEC 7.46] (AB7388)

Flow cytometric analysis of / fixed / permeabilized B16-F0 (Mouse melanoma epithelial cell-like, Left) / bEnd.3 (Mouse brain endothelioma, Right) cells labelling CD31 with ab7388 at 1/1000 dilution (0.1μg) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Negative control : B16-F0.Gated on viable cells.

Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [MEC 7.46] (AB7388)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [MEC 7.46] (AB7388)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized bEnd.3 cells labelling CD31 with ab7388 at 1/50 (19.32 μg/ml) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in bEnd.3 cell line. Negative control : B16-F0 (PMID : 8082649) ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

関連する標識済み抗体及び組成の異なる製品 (5)

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

MEC 7.46

アイソタイプ

IgG1

軽鎖のタイプ

kappa

キャリアフリー

No

交差種

Mouse

アプリケーション

Flow Cyt, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

The antibody reacts with the murine form of the Platelet-Endothelial Cell Adhesion Molecule. The reactivity of the antibody is restricted to the isoform of the molecule that is selectively expressed by endothelial cells. The antibody precipitates a 130 kDa molecule present on the membrane of endothelial cell presents on all mouse blood vessels both in normal and inflamed or tumor tissues. The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/1000", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
style
left-column

製品の詳細

What is this antibody validated in?
Anti-CD31 antibody [MEC 7.46] (ab7388) is a rat recombinant monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.

Trusted by the scientific community
Anti-CD31 [MEC 7.46] (ab7388) was first used in a scientific publication in 2002 and has been cited over 130 times in peer-reviewed journals.

Reviewed by scientists
Anti-CD31 [MEC 7.46] (ab7388) has over 5 independent reviews from customers.

Other related products
We have a range of other formats of antibody clone [MEC 7.46] also available for your convenience: ab7388, Carrier free - ab264486, Alexa Fluor® 488 - ab307133, Alexa Fluor® 647 - ab307262, Alexa Fluor® 594 - ab307336, PE - ab314282

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (By similarity). Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (By similarity). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (By similarity). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (By similarity). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (By similarity). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (By similarity). Modulates bradykinin receptor BDKRB2 activation (By similarity). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (By similarity). Induces susceptibility to atherosclerosis (PubMed : 19048083).
See full target information Pecam1
style
left-column

文献 (166)

Recent publications for all applications. Explore the full list and refine your search

Stem cell reviews and reports : PubMed40987919

2025

Investigating the Therapeutic Efficacy of Quality-Controlled, miR-146a-5p-Enriched Small Extracellular Vesicles Derived From MSCs Against Idiopathic Pulmonary Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Wang,Lingjiao Meng,Qiuhong Wang,Ruixue Rong,Yu Zhang,Xiaohui Zhao,Chen Liang,Huizhen Guo,Li Deng,Zengqi Tan,Feng Guan,Yi Tan

Nature communications 16:6357 PubMed40640166

2025

Regulation of partial endothelial-to-mesenchymal transition by circATXN1 in ischemic diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Li,Zhe Zheng,Yanze Li,Siyuan Fan,Lingyao Kong,Wanrong Fu,Zhonggen Li,Jianchao Zhang,Shuang Li,Zongtao Liu,Chao Liu,Jinhua Cao,Zhenxuan Hao,Lili Xiao,Youyou Du,Xiaofang Wang,Lu Gao

Glia 73:2057-2076 PubMed40631759

2025

SUR1-TRPM4 Regulates Aquaporin-4 Subcellular Localization by Astrocytic Endfeet Calcium Signals Following Status Epilepticus.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Yang,Zhenzhou Lin,Mingjia Yu,Yongchuan Li,Jiancong Chen,Yuanchi Liu,Kaibin Huang,Suyue Pan

Biology direct 20:36 PubMed40140903

2025

Circulating extracellular vesicles regulate ELAVL1 by delivering miR-133a-3p which affecting NLRP3 mRNA stability inhibiting PANoptosome formation.

Applications

Unspecified application

Species

Unspecified reactive species

Deliang Wang,Zheng Dai,Lu Jiang,Ke Liu

Stem cell research & therapy 16:43 PubMed39901221

2025

Intranasal delivery of hMSC-derived supernatant for treatment of ischemic stroke by inhibiting the pro-inflammatory polarization of neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Yixiang Jiang,Ning Wang,Jingyi Liu,Haoran Ren,Wenkang Jiang,Yanting Lei,Xidan Fu,Miao Hao,Xiujuan Lang,Yumei Liu,Xijun Liu,Rui Li,Hulun Li

iScience 28:111548 PubMed39897939

2025

Neonatal infection with promotes autism-like phenotypes in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Eoin O'Neill,Lucy Curham,Caitlín Ní Chasaide,Síofra O'Brien,Gavin McManus,Barry Moran,Keith Rubin,Steven Glazer,Marina A Lynch,Kingston H G Mills

Bioactive materials 45:181-200 PubMed39651397

2024

Engineering aortic valves via transdifferentiating fibroblasts into valvular endothelial cells without using viruses or iPS cells.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Tang,Fuxiang Wei,Weihua Qiao,Xing Chen,Chenyang Ji,Wanzhi Yang,Xinyu Zhang,Sihan Chen,Yanyan Wu,Mingxing Jiang,Chenyu Ma,Weiqiang Shen,Qi Dong,Hong Cao,Minghui Xie,Ziwen Cai,Li Xu,Jiawei Shi,Nianguo Dong,Junwei Chen,Ning Wang

Theranostics 14:7219-7240 PubMed39629129

2024

Gut microbiota depletion and FXR inhibition exacerbates zonal hepatotoxicity of sunitinib.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhao,Yingmei Lu,Jingyi Duan,Dan Du,Qianlun Pu,Fei Li

Cell reports. Medicine 5:101795 PubMed39454569

2024

A humanized monoclonal antibody targeting an ectonucleotidase rescues cardiac metabolism and heart function after myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Shen Li,Bo Tao,Jijun Wan,Enca Montecino-Rodriguez,Ping Wang,Feiyang Ma,Baiming Sun,Yiqian Gu,Sivakumar Ramadoss,Lianjiu Su,Qihao Sun,Johanna Ten Hoeve,Linsey Stiles,Jeffrey Collins,R Michael van Dam,Mikayla Tamboline,Richard Taschereau,Orian Shirihai,Douglas B Kitchen,Matteo Pellegrini,Thomas Graeber,Kenneth Dorshkind,Shili Xu,Arjun Deb

Nature communications 15:8430 PubMed39341842

2024

The urotensin II receptor triggers an early meningeal response and a delayed macrophage-dependent vasospasm after subarachnoid hemorrhage in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Pedard,Lucie Prevost,Camille Carpena,Brian Holleran,Laurence Desrues,Martine Dubois,Celeste Nicola,Roxane Gruel,David Godefroy,Thomas Deffieux,Mickael Tanter,Carine Ali,Richard Leduc,Laurent Prézeau,Pierrick Gandolfo,Fabrice Morin,Olivier Wurtz,Thomas Bonnard,Denis Vivien,Hélène Castel
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB222783

Anti-CD31 antibody [EPR17260-263]

Recombinant|RabMAb|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [EPR17260-263] (AB222783)

  • 5
  • 11
Primary Antibodies

AB134168

Anti-CD31 antibody [EP3095]

RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] (AB134168)

  • 4
  • 7
Primary Antibodies

AB182981

Anti-CD31 antibody [EPR17259]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-CD31 antibody [EPR17259] (AB182981)

  • 5
  • 22
Primary Antibodies

AB76533

Anti-CD31 antibody [EPR3094]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] (AB76533)

  • 4
  • 12
Primary Antibodies

AB207090

Anti-CD31 antibody [EPR3094] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] - BSA and Azide free (AB207090)

  • 5
  • 2
Primary Antibodies

AB226157

Anti-CD31 antibody [EP3095] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EP3095] - BSA and Azide free (AB226157)

  • 0
  • 0
Primary Antibodies

AB199734

Biotin Anti-CD31 antibody [EPR3094]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-CD31 antibody [EPR3094] (AB199734)

  • 0
  • 0
Primary Antibodies

AB218582

Alexa Fluor® 647 Anti-CD31 antibody [EPR3094]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD31 antibody [EPR3094] (AB218582)

  • 0
  • 0

組合せ製品

Assay Kits

AB204527

Mouse CD31 ELISA Kit (PECAM1)

Recombinant|SimpleStep

Sandwich ELISA - Mouse CD31 ELISA Kit (PECAM1) (AB204527)

  • 0
  • 0
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

  • 5
  • 49

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com