Anti-CD31 抗体

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB28364)

Immunohistochemical analysis of formalin fixed paraffin embedded human lung labelling CD31 with ab28364 at a concentration of 0.40 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab28364 Anti-CD31 antibody was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB28364)

ab28364 at 1/50 dillution staining CD31 in human tonsil tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

• IHC-P

AbReview76203****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB28364)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD31 with ab28364 at 1/50 dilution (30 minutes at 23°C). 10% Neutral buffered formalin used as fixative. Heat mediated antigen retrieval was performed at 95°C for 20 minutes. Blocking performed using 2.5% serum for 20 minutes at 23°C. HRP-conjugated Goat Anti-rabbit was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Mr. Victor Bernal.

• IHC-P

AbReview76110****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB28364)

Immunohistochemistry (PFA-fixed paraffin-embedded sections) analysis of human palatine tonsil tissue labelling CD31 with ab28364 at 1/50 dilution (15 hours at 4°C). Heat mediated antigen retrieval was performed using Tris/EDTA buffer. Blocking performed with 5% normal goat serum for 1 hour at 21°C. HRP-conjugated Goat Anti-Rabbit was used as the secondary antibody.

This image is courtesy of an anonymous Abreview.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB28364)

Immunohistochemical analysis of formalin fixed paraffin embedded human lung labelling CD31 with ab28364 at a concentration of 0.50 µg/ml. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with a Bond™ Polymer Refine Detection kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.ab28364 Anti-CD31 antibody was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin. Image inset shows absence of staining in secondary antibody only control.

• IHC-P

AbReview78290****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB28364)

Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed human skin tissue. Stained with ab28364 at 1/100 dilution. Secondary antibody used was Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG at 1/300 dilution. Blocking was done with 5% BSA for 30 minutes at 21°C. The sample was incubated with the primary antibody for 30 minutes at 4°C. Antigen retrieval method was heat mediated with citrate buffer pH 6

This image is courtesy of an anonymous Abreview

• IHC

CiteAb

Immunohistochemistry - Anti-CD31 antibody (AB28364)

Immunohistochemistry-paraffin using Anti-CD31 antibody, ab28364. Publication image from Liao, Y. et al., 2020, Theranostics, 32373208. Legend direct from paper.

The down-regulation of HOXA5 predicts poor prognosis and negatively correlates with angiogenesis in HCC. (A) Representative IHC staining images of HOXA5 in HCC and adjacent non-tumor liver tissues. Scale bar, 500 µm. (B) HOXA5 expression was significantly decreased in HCC tissues. Quantification of HOXA5 levels according to IHC scores. (C) Kaplan-Meier plot revealed shorter overall and recurrence-free survival in the lower HOXA5 group. Based on the minimum p-value approach, the 49th percentile of the HOXA5 level in 449 HCC tissues was chosen as the cut-off value for dividing the HOXA5-low level group (n = 220) from the HOXA5-high level group (n = 229). (D) (E) and (F) HOXA5 was negatively correlated with angiogenesis markers CD31, CD34 in HCC. (D) Representative staining images of HOXA5 and CD31, CD34 are shown. (E) The expression of CD31 and CD34 in HOXA5-low and HOXA5-high group, respectively. The median IHC scores were chosen as the cut-off value for separating the low and high groups (n = 314). (F) Correlations between HOXA5 and CD31, CD34 in HCC were assessed using the Pearson correlation and linear regression analytics (n = 314). * p < 0.05, *** p < 0.001.

• IHC

CiteAb

Immunohistochemistry - Anti-CD31 antibody (AB28364)

Immunohistochemistry-paraffin using Anti-CD31 antibody, ab28364. Publication image from Liao, Y. et al., 2020, Theranostics, 32373208. Legend direct from paper.

The down-regulation of HOXA5 promotes HCC tumorigenicity and angiogenesis in vivo. (A) HOXA5 down-regulation promoted the growth of Huh7 xenograft tumors. HOXA5 down-regulated and control Huh7 cells were subcutaneously injected into nude mice (n = 8). The nude mice were sacrificed on day-28 after inoculation and the tumor were harvested. (B) The tumor weight on day-28 after inoculation are presented. (C) The tumor growth curve was measured every week after inoculation. The mean tumor volume was significantly increased in the HOXA5-downregulated group. (D) HOXA5 down-regulation elevated the expression of angiogenesis markers. Immunohistochemical expression of angiogenesis markers CD31, CD34 and VEGF were detected on serial sections of tumor samples. Scale bar, 100 µm (100x), 25 µm (400x). (E) The staining intensity of CD31, CD34, and VEGF from tumor samples were shown.Data are presented as their mean ± SD. Statistical analysis was performed with Student's t-test. *p < 0.05; ***p < 0.001.

• IHC

CiteAb

Immunohistochemistry - Anti-CD31 antibody (AB28364)

Immunohistochemistry-paraffin using Anti-CD31 antibody, ab28364. Publication image from Liao, Y. et al., 2020, Theranostics, 32373208. Legend direct from paper.

miR-130b-3p promotes tumor angiogenesis in vitro and in vivo. (A) TCM from miR-130b-3p transfected HCC cells induced tube formation of HUVECs. Representative images of capillary-like structures and the number of branch points of HUVECs are shown. (B) Effect of miR-130b-3p on vascularization in the CAM angiogenesis model. Filter discs soaked with TCM were loaded on the CAMs of Day 8 chick embryos. After incubation for 5 days, the area under and surround the filter was fixed and photographed. Representative images of neovascularization and the number of new blood vessels are shown. (C) HUVECs proliferation was promoted by the TCM from miR-130b-3p transfected HCC cells. HUVEC cells were seeded on 6-well plate with a density of 3 x 105 cells per well and were cultured with SFM supplemented with 20% FBS and 0.3% EGF for 6 hours and shifted to TCM from indicated cells and cultured for additional 24 h. The number of HUVEC cells was counted using the ScepterTM Handheld Automated Cell Counter. Results were based on six independent experiments. (D) The mRNA level of angiogenesis relevant genes was upregulated by miR-130b-3p. The expression of MMP9, FGF2, VEGFA, VEGFC, PDGFA, PDGFC in Huh7 cells transfected with miR-130b-3p or vector were determined by qRT-PCR. Results were based on at least three independent experiments. Data are presented as their mean ± SD. (E) miR-130b-3p overexpressed and control BEL-7402 cells were subcutaneously injected into nude mice (n = 8). The nude mice were sacrificed on day 30 after inoculation and the tumor were harvested. (F) The tumor weight on day-30 after inoculation are presented (left). The tumor growth curve was measured every 3 days for 30 days after inoculation. The mean tumor volume was significantly increased in the miR-130b-3p overexpression group (right). (G) Representive immunohistochemical images of endothelial markers CD31, CD34 and VEGF on serial sections of tumor samples are shown. Scale bar, 100 µm. (H) The staining intensity of CD31, CD34, and VEGF from tumor samples are shown. Data are presented as their mean ± SD. Statistical analysis was performed using the Student's t-test. * p < 0.05, **p < 0.01, ***p < 0.001.