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AB303539

Anti-CD30-L 抗体 [EPR24237-61] - BSA and Azide free

Anti-CD30-L antibody [EPR24237-61] - BSA and Azide free

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Rabbit Recombinant Monoclonal CD30-L antibody. Carrier free. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.

別名を表示する

CD153, CD30L, CD30LG, TNFSF8, Tumor necrosis factor ligand superfamily member 8, CD30 ligand, CD30-L

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CD30-L antibody [EPR24237-61] - BSA and Azide free (AB303539)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD30-L antibody [EPR24237-61] - BSA and Azide free (AB303539)

This data was developed using ab303538, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC (human Peripheral blood mononuclear cell) cells labelling CD30-L with ab303538 at 1/50 (10.42 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and membranous staining in subsets of human PBMC treated with calcium ionomycin (200 ng/ml) for 24 h. is observed. Anti-rat CD3 mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Flow Cytometry - Anti-CD30-L antibody [EPR24237-61] - BSA and Azide free (AB303539)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD30-L antibody [EPR24237-61] - BSA and Azide free (AB303539)

This data was developed using ab303538, the same antibody clone in a different buffer formulation.Flow cytometric analysis of / fixed / permeabilized Human peripheral blood mononuclear cell (PBMC) treated with 50ng/ml PMA and 200 ng/ml calcium ionomycin for 24 hours cells labelling CD30-L with ab303538 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Cells were stained with rabbit IgG or ab303538. Then stained with anti-CD3 conjugated to Alexa Fluor® 647.

Flow Cytometry - Anti-CD30-L antibody [EPR24237-61] - BSA and Azide free (AB303539)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD30-L antibody [EPR24237-61] - BSA and Azide free (AB303539)

This data was developed using ab303538, the same antibody clone in a different buffer formulation.Flow cytometric analysis of / fixed / permeabilized Human peripheral blood mononuclear cell (PBMC) treated with 50ng/ml PMA and 200 ng/ml calcium ionomycin for 24 hours cells labelling CD30-L with ab303538 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR24237-61

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

Flow Cyt, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Assay Kits

AB252362

Human CD30-L ELISA Kit (TNFSF8)

Recombinant|SimpleStep

ELISA - Human CD30-L ELISA Kit (TNFSF8) (AB252362)

  • 0
  • 0
Sample Prep & Detection Kits

AB269823

Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link®

Immunocytochemistry - Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link® (AB269823)

  • 5
  • 2
Primary Antibodies

AB192033

Anti-Nectin-4 antibody [EPR15613-68]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-Nectin-4 antibody [EPR15613-68] (AB192033)

  • 0
  • 0
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
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Reactivity data

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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
See full target information TNFSF8
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com