Name: Anti-CD3 epsilon antibody [EP449E]
Brand: RabMAb
SKU: ab52959
Rating: 5 (3 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP449E] to CD3 epsilon
Suitable for: WB, IHC-P, IP
Reacts with: Human

Carrier Free

製品名

Anti-CD3 epsilon antibody [EP449E]
CD3 epsilon 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EP449E] to CD3 epsilon
由来種

Rabbit
特異性

This antibody reacts with CD3 epsilon. It does not cross-react with other subunits of CD3.
アプリケーション

適用あり: WB, IHC-P, IPmore details
種交差性

交差種: Human
免疫原

Synthetic peptide corresponding to Human CD3 epsilon aa 150-250. Derived from the cytoplasmic domain.
Database link: P07766
ポジティブ･コントロール
- Jurkat cell lysate and human spleen tissue. IP: Jurkat whole cell lysate.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EP449E
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608)
- Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Jurkat membrane extract lysate (ab30130)
Recombinant Protein
- Recombinant human CD3 epsilon protein (Active) (ab167755)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab52959の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB	(1)	1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P	(2)	1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP		1/20.

特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/20.

機能

The CD3 complex mediates signal transduction.
配列類似性

Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain.
細胞内局在

Membrane.
Target information above from: UniProt accession P07766 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 916 Human
- Omim: 186830 Human
- SwissProt: P07766 Human
- Unigene: 3003 Human
別名
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
- CD3e antigen epsilon polypeptide (TiT3 complex) antibody
- CD3E antigen epsilon polypeptide antibody
- CD3E antigen, epsilon subunit antibody
- CD3e molecule epsilon antibody
- CD3e molecule, epsilon (CD3 TCR complex) antibody
- CD3e molecule, epsilon (CD3-TCR complex) antibody
- CD3E_HUMAN antibody
- IMD18 antibody
- T cell antigen receptor complex epsilon subunit of T3 antibody
- T cell surface antigen T3/Leu 4 epsilon chain antibody
- T cell surface glycoprotein CD3 epsilon chain antibody
- T-cell surface antigen T3/Leu-4 epsilon chain antibody
- T-cell surface glycoprotein CD3 epsilon chain antibody
- T3E antibody
- TCRE antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

ab52959 at 1/250-1/500 dilution staining CD3 epsilon in human spleen by Immunohistochemistry, Paraffin embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-CD3 epsilon antibody [EP449E] (ab52959)

All lanes : Anti-CD3 epsilon antibody [EP449E] (ab52959) at 1/1000 dilution (purified)

Lane 1 : Jurkat( Human T cell leukemia T lymphocyte )membrane fraction cell lysate
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) without membrane fraction cell lysate
Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 23 kDa
Observed band size: 23 kDa

Exposure time: 20 seconds

Blocking/Dilution buffer and concentration: 5% NFDM/TBST
Immunoprecipitation - Anti-CD3 epsilon antibody [EP449E] (ab52959)

Purified ab52959 at 1/20 dilution (1µg) immunoprecipitating CD3 epsilon in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab52959 + Jurkat whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52929 in Jurkat whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 23 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

ab52959 showing positive staining in Normal tonsil tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

ab52959 showing positive staining in Normal spleen tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

ab52959 showing negative staining in Normal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

ab52959 showing negative staining in Normal kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

ab52959 showing negative staining in Skeletal muscle tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-CD3 epsilon antibody [EP449E] (ab52959)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab52959 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab52959 は 16 報の論文で使用されています。

Gu J et al. A Low-Cost Modular Imaging System for Rapid, Multiplexed Immunofluorescence Detection in Clinical Tissues. Int J Mol Sci 24:N/A (2023). PubMed: 37108170
Simonson PD et al. Tyramide-conjugated DNA barcodes enable signal amplification for multiparametric CODEX imaging. Commun Biol 5:627 (2022). PubMed: 35754060
Schaadt NS et al. Graph-based description of tertiary lymphoid organs at single-cell level. PLoS Comput Biol 16:e1007385 (2020). PubMed: 32084130
Kim D et al. Somatic mTOR mutation in clonally expanded T lymphocytes associated with chronic graft versus host disease. Nat Commun 11:2246 (2020). PubMed: 32382059
Rao A et al. Cytokines regulate the antigen-presenting characteristics of human circulating and tissue-resident intestinal ILCs. Nat Commun 11:2049 (2020). PubMed: 32341343

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-4 of 4 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Skin)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate

Permeabilization

Specification

Skin

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 17 2022

Application

Western blot

Sample

Human Cell lysate - whole cell (T-cells)

Gel Running Conditions

Reduced Denaturing (4-20% Tris HCl)

Loading amount

7.69 µg

Specification

T-cells

Blocking step

Licor intercept TBS as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 13 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (distal colon)

Specification

distal colon

Fixative

Paraformaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate buffer

Permeabilization

Blocking step

Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Isabel Romero Calvo

Verified customer

投稿 Jan 11 2013

Question

Antikörper, der humane T Zellen erkennt aber NICHT T Zellen aus der Ratte in IHC-P.

Abcam community

Verified customer

Asked on Sep 22 2011

Answer

Vielen dank fuer Ihren Anruf. Ich habe nun ein paar Antikoerper fuer Sie herrausgesucht, die nur humane T Zellen erkennen und nicht T Zellen aus der Ratte und fuer IHC-P getestet und garantiert sind. Bitte folgen Sie den jeweiligen Links um zu den Datenblaetern mit mehr Informatinen zugelangen. ab32327 Vielen Dank für Ihren Anruf. Ich habe nun ein paar Antikörper für Sie herausgesucht, die nur humane T Zellen erkennen und nicht T Zellen aus der Ratte und für IHC-P getestet und garantiert sind. Bitte folgen Sie den jeweiligen Links um zu den Datenblättern mit mehr Informationen zugelangen. ab32327 Click here (or use the following: https://www.abcam.com/CD3-epsilon-antibody-E292-ab32327.html). ab33524 Click here (or use the following: https://www.abcam.com/CD3-epsilon-antibody-PS1-ab33524.html). ab52959 Click here (or use the following: https://www.abcam.com/CD3-epsilon-antibody-EP449E-ab52959.html). ab109531 Click here (or use the following: https://www.abcam.com/CD3D-antibody-EP4426-ab109531.html). Ich hoffe, diese Liste hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder zu melden, falls Sie weitere Fragen haben. Click here (or use the following: https://www.abcam.com/CD3-epsilon-antibody-E292-ab32327.html). ab33524 Click here (or use the following: https://www.abcam.com/CD3-epsilon-antibody-PS1-ab33524.html). ab52959 Click here (or use the following: https://www.abcam.com/CD3-epsilon-antibody-EP449E-ab52959.html). ab109531 Click here (or use the following: https://www.abcam.com/CD3D-antibody-EP4426-ab109531.html). Ich hoffe, diese Liste hilft Ihnen weiter. Bitte zoegern Sie nicht, sich wioeder zu melden, falls Sie weitere Fragen haben.

Abcam Scientific Support

Answered on Sep 22 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (16)

レビューと Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 epsilon antibody [EP449E]

Western blot abreview for Anti-CD3 epsilon antibody [EP449E]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 epsilon antibody [EP449E]