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リコンビナントRabMAb

Anti-CD3 epsilon 抗体 [E272] (ab32186)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [E272] (ab32186)
  • Immunoprecipitation - Anti-CD3 epsilon antibody [E272] (ab32186)
  • Anti-CD3 epsilon antibody [E272] (ab32186)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E272] to CD3 epsilon
  • Suitable for: IHC-P, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-CD3 epsilon antibody [E272]
    CD3 epsilon 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [E272] to CD3 epsilon

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, IPmore details
    適用なし: Flow Cyt or WB

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human CD3 epsilon aa 150-250 (C terminal). The exact sequence is proprietary.

  • ポジティブ･コントロール

    • IP: Jurkat cell lysate IHC-P: Human lymph node.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab32186の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/50.
特記事項
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/50.
追加情報
Is unsuitable for Flow Cyt or WB.

ターゲット情報

  • 機能

    The CD3 complex mediates signal transduction.

  • 配列類似性

    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD3 epsilon antibody
      • CD3e antibody
      • CD3e antigen antibody
      • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3E antigen, epsilon subunit antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule, epsilon (CD3 TCR complex) antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3E_HUMAN antibody
      • IMD18 antibody
      • T cell antigen receptor complex epsilon subunit of T3 antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T3E antibody
      • TCRE antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [E272] (ab32186)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [E272] (ab32186)

      Ab32186, at a 1/100 dilution, staining CD3 epsilon in paraffin embedded human lymph node tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-CD3 epsilon antibody [E272] (ab32186)
      Immunoprecipitation - Anti-CD3 epsilon antibody [E272] (ab32186)
      Western blot analysis of immunoprecipitation pellets from Jurkat cell lysate or PBS (lane 2: negative control), using ab32186 at a 1/50 dilution. The predicted band size of 23 kDa was observed.
    • Anti-CD3 epsilon antibody [E272] (ab32186)
      Anti-CD3 epsilon antibody [E272] (ab32186)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab32186 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab32186 は論文での使用が確認できていません。

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