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AB215267

Anti-CD3 抗体 [UCH-T1] - BSA and Azide free

Anti-CD3 antibody [UCH-T1]

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(1 Publication)

Mouse Monoclonal CD3d antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD3D protein.

別名を表示する

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

2 Images
Flow Cytometry - Anti-CD3 antibody [UCH-T1] (AB215267)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD3 antibody [UCH-T1] (AB215267)

Flow cytometric analysis of the dose-dependency (D, F, H) of ab215267 binding to live human PBMCs (B). The gating strategy is shown for control puposes (A, B, C, E, G).

Western blot - Anti-CD3 antibody [UCH-T1] (AB215267)
  • WB

Unknown

Western blot - Anti-CD3 antibody [UCH-T1] (AB215267)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-CD3 antibody [UCH-T1] (ab215267) at 3 µg/mL

Lane 1:

Jurkat cell lysate (in RIPA buffer) at 35 µg

Lane 2:

Molt4 cell lysate (in RIPA buffer) at 35 µg

Predicted band size: 19 kDa

Observed band size: 23 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

UCH-T1

アイソタイプ

IgG1

キャリアフリー

No

交差種

Human

アプリケーション

WB, Flow Cyt

applications

免疫原

Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information.

P04234

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代替製品

Primary Antibodies

AB5690

Anti-CD3 epsilon antibody

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody (AB5690)

  • 4
  • 46
Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

  • 4
  • 80
Primary Antibodies

AB21703

Anti-CD3 epsilon antibody [SP7], prediluted

BOND RX™ Validated|RabMAb|Lab Essentials|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7], prediluted (AB21703)

  • 5
  • 1
Primary Antibodies

AB246799

Anti-CD3 antibody [SP162] - Low endotoxin, Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (AB246799)

  • 0
  • 0
Primary Antibodies

AB245731

Anti-CD3 antibody [SP162] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD3 antibody [SP162] - BSA and Azide free (AB245731)

  • 5
  • 3
Primary Antibodies

AB135372

Anti-CD3 antibody [SP162]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] (AB135372)

  • 4
  • 10
Primary Antibodies

AB16044

Anti-CD3 antibody

Immunoprecipitation - Anti-CD3 antibody (AB16044)

  • 4
  • 2

組合せ製品

Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

  • 4
  • 80
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB237709

Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] (AB237709)

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "3-4 µg/mL", "WB-species-notes": "<p></p>" } } }
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製品の詳細

Clone UCH-T1 is considered a pan T-cell marker - it can be used for the detection of T cell populations in peripheral blood and lymph nodes and the categorisation of T versus B cell lymphomas and leukaemias. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukaemia cells, Sézary leukaemias and approximately 70% of acute lymphoblastic leukaemias of T cell origin.

ab215267 is a recombinant monoclonal antibody produced from the original monoclonal with variable regions (i.e. specificity) from the hybridoma.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
Preservative: 0.02% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).
See full target information CD3D
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

General physiology and biophysics 39:229-237 PubMed32525816

2020

The circRNA-MYLK plays oncogenic roles in the Hep-2 cell line by sponging microRNA-145-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Chen,Yanmei Wang,Yanmei Li,Xuechan Li,Tiejun Yuan,Shuqin Yang,Xiaoyan Sun
View all publications
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Abcam product promise

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