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  1. Link

    products/primary-antibodies/cd3-antibody-ps1-ab699.pdf

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Immunology Adaptive Immunity T Cells CD
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Anti-CD3 抗体 [PS1] (ab699)

  • Datasheet
  • SDS
Reviews (12)Q&A (12)References (39)

Product price, shipping and contact information

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出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Mouse monoclonal [PS1] to CD3
  • Suitable for: IHC-Fr, IHC-P, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG2a

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-CD3 antibody [SP162] (ab135372)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-CD3 antibody [PS1]
    CD3 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [PS1] to CD3
  • 由来種

    Mouse
  • 特異性

    This antibody, when used in conjunction with UCHL-1, is regarded as a reliable pan T-cell marker for immunophenotyping of lymphomas (See reference). Exceptions include some of the more aggressive, high-grade T cell lymphomas, which may not express detectable antigen. This antibody does not react with B-cells.
  • アプリケーション

    適用あり: IHC-Fr, IHC-P, ICC/IF, WBmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Fusion protein corresponding to CD3. Prokaryotic fusion protein corresponding to the epsilon chain of CD3

  • ポジティブ・コントロール

    • Tonsil or T-cell lymphoma. IHC-P: Human colon tissue and colorectal mucosa tissue. IHC-Fr: Human skin tissue.
  • 特記事項

    This antibody works well when used in combination with ab972 - Antigen retrieval solution - heat mediated high pH 9.5.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.09% Sodium azide
    Constituents: 97.5% Water, 0.99% BSA, 0.33% Tris HCl, 0.04% Tween, 0.007% Hydrochloric acid
  • Concentration information loading...
  • 精製度

    Affinity purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    PS1
  • ミエローマ

    unknown
  • アイソタイプ

    IgG2a
  • 軽鎖の種類

    unknown
  • 研究分野

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Mouse CD3 protein (Fc Chimera) (ab220543)
    • Recombinant Human CD3 epsilon protein (Fc Chimera His Tag) (ab220590)
  • Related Products

    • Human CD3 peptide (ab16206)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab699の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr (1)
Use at an assay dependent concentration.
IHC-P (3)
Use at an assay dependent concentration.
ICC/IF (3)
Use at an assay dependent concentration.
WB (4)
Use at an assay dependent concentration. Predicted molecular weight: 19 kDa. PubMed: 21654633
特記事項
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 19 kDa. PubMed: 21654633

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (39)

ab699 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab699 は 39 報の論文で使用されています。

  • Gabanella F  et al. Reduced expression of secretogranin VGF in laryngeal squamous cell carcinoma. Oncol Lett 27:37 (2024). PubMed: 38108073
  • Kida Y  et al. Urokinase-type plasminogen activator blockade ameliorates experimental colitis in mice. Sci Rep 13:2899 (2023). PubMed: 36806262
  • Gao Y  et al. Hyperprogression of cutaneous T cell lymphoma after anti-PD-1 treatment. JCI Insight 8:N/A (2023). PubMed: 36649072
  • van den Bosch AMR  et al. Ultrastructural Axon-Myelin Unit Alterations in Multiple Sclerosis Correlate with Inflammation. Ann Neurol 93:856-870 (2023). PubMed: 36565265
  • Jiang H  et al. Revealing the transcriptional heterogeneity of organ-specific metastasis in human gastric cancer using single-cell RNA Sequencing. Clin Transl Med 12:e730 (2022). PubMed: 35184420
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 24 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [PS1]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Sarcoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
Human Sarcoma
Blocking step
innovex Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 09 2015

Western blot abreview for Anti-CD3 antibody [PS1]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gels)
Sample
Human Cell lysate - whole cell (Jurkats and PBMCS)
Specification
Jurkats and PBMCS
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 19 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-CD3 antibody [PS1]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Lymphocytes)
Specification
Lymphocytes
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 06 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [PS1]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 06 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-CD3 antibody [PS1]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human peripheral blood mononuclear cells)
Specification
Human peripheral blood mononuclear cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 25 2013

Western blot abreview for Anti-CD3 antibody [PS1]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Spleen whole extract)
Loading amount
100 µg
Specification
Spleen whole extract
Gel Running Conditions
Reduced Denaturing (Tris-Glycine -10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 28 2012

Western blot abreview for Anti-CD3 antibody [PS1]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Peripheral blood mononuclear cells)
Loading amount
20 µg
Specification
Peripheral blood mononuclear cells
Gel Running Conditions
Reduced Denaturing (Tris-Glycine -10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 31 2012

Western blot abreview for Anti-CD3 antibody [PS1]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human peripheral blood mononuclear cells)
Loading amount
25 µg
Specification
Human peripheral blood mononuclear cells
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 30 2012

Question

Interested in CD3 ab without sodium azide.

Read More

Abcam community

Verified customer

Asked on May 07 2013

Answer

Here is a link to our protocol for sodium azide removal

https://www.abcam.com/index.html?pageconfig=resource&rid=13869

Read More

Abcam Scientific Support

Answered on May 07 2013

Question

I ordered the following antibody which I will be using for IHC-P:

Anti-CD3 antibody [PS1] (ab699)

To use the corresponding isotype control with the correct concentration I need to know how the Anti-CD3 antibody is concentrated.

Could you please e-mail me the concentration or if the exact concentration is not known which concentration it roughly has?

Thank you in advance.

Read More

Abcam community

Verified customer

Asked on Jan 08 2013

Answer

Vielen Dank für Ihre Anfrage.

Die Konzentration für den ab699 Anti-CD3 antibody [PS1] mit dem Lot GR95849-1, welches Sie erhalten haben, beträgt 80ug/ml.

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

Answered on Jan 08 2013

1-10 of 24 Abreviews or Q&A

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