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Anti-CD3 抗体 [MEM-57] (ab8090)

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Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)
  • Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)
  • Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)
  • Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)

Key features and details

  • Mouse monoclonal [MEM-57] to CD3
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-CD3 antibody [MEM-57]
    CD3 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [MEM-57] to CD3

  • 由来種

    Mouse

  • 特異性

    This antibody reacts with epsilon-gamma and epsilon-delta dimers of human CD3 complex, a part of a bigger multisubunit complex of the T cell receptor for antigen (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes. It reacts more strongly with blood gamma-delta T lymphocytes than with alpha-beta T lymphocytes.

  • アプリケーション

    適用あり: Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Tissue, cells or virus corresponding to Human CD3. Human thymocytes and T lymphocytes

  • 特記事項

    Activates T cells in vitro. This antibody is a highly effective immunosuppressive agent. In this it is similar to OKT3.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • 特記事項（精製）

    This product has been purified from tissue culture supernatant.

  • 一次抗体 備考

    Activates T cells in vitro. This antibody is a highly effective immunosuppressive agent. In this it is similar to OKT3.

  • ポリ/モノ

    モノクローナル

  • クローン名

    MEM-57

  • アイソタイプ

    IgG2a

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab8090の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use a concentration of 2 - 5 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
特記事項
Flow Cyt
Use a concentration of 2 - 5 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 

画像

  • Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)
    Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)

    Human peripheral blood lymphocytes stained with ab8090 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab8090, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

  • Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)
    Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)

    Flow cytometry analysis of human peripheral whole blood labeling CD3 with ab8090 at 0.33 μg/mL (GAM APC).

  • Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)
    Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)

    Flow cytometry analysis of human peripheral whole blood labeling CD3 with ab8090 at 0.33 μg/mL (GAM APC). Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed).

  • Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)
    Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)
    Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD3 (MEM-57) Dy161. Gated on singlets.

参考文献 (8)

ab8090 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab8090 は 8 報の論文で使用されています。

  • Garrett SM  et al. Induction of a Th17 Phenotype in Human Skin-A Mimic of Dermal Inflammatory Diseases. Methods Protoc 2:N/A (2019). PubMed: 31164624
  • Savitski MM  et al. Multiplexed Proteome Dynamics Profiling Reveals Mechanisms Controlling Protein Homeostasis. Cell 173:260-274.e25 (2018). PubMed: 29551266
  • Fehrholz M  et al. Impact of the New Generation Reconstituted Surfactant CHF5633 on Human CD4+ Lymphocytes. PLoS One 11:e0153578 (2016). PubMed: 27077658
  • Lipp AM  et al. Lck mediates signal transmission from CD59 to the TCR/CD3 pathway in Jurkat T cells. PLoS One 9:e85934 (2014). Human . PubMed: 24454946
  • Giese S & Marsh M Tetherin can restrict cell-free and cell-cell transmission of HIV from primary macrophages to T cells. PLoS Pathog 10:e1004189 (2014). Flow Cyt . PubMed: 24991932
  • Bergamini G  et al. A selective inhibitor reveals PI3K? dependence of T(H)17 cell differentiation. Nat Chem Biol 8:576-82 (2012). PubMed: 22544264
  • Hilgert I  et al. Therapeutic in vivo use of the A1-CD3 monoclonal antibody. Transplantation 55:435-8 (1993). PubMed: 8434399
  • Horejsí V  et al. Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha) 34:23-34 (1988). PubMed: 2968928

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