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AB11089

Anti-CD3 抗体 [CD3-12]

Anti-CD3 antibody [CD3-12]

5

(18 Reviews)

|

(104 Publications)

Anti-CD3 antibody [CD3-12] (ab11089) is a rat monoclonal antibody detecting CD3 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 100 publications
- Trusted since 2004

別名を表示する

CD3e, T3E, CD3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (AB11089)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (AB11089)

Immunohistochemical analysis of paraffin-embedded Mouse spleen and kidney tissue labeling CD3 with ab11089 at 5 µg/mL, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (AB11089)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (AB11089)

Flow cytometric analysis of human peripheral blood mononuclear cells (PMBCs) labelling CD3 with ab11089 at 5 µg/mL (right) compared with a Rat IgG1k isotype control (left).

Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (AB11089)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (AB11089)

Flow cytometric analysis of Mouse spleen cells labelling CD3 with ab11089 at 1 µg/mL (right) compared with a Rat IgG1k isotype control (left).

Western blot - Anti-CD3 antibody [CD3-12] (AB11089)
  • WB

Lab

Western blot - Anti-CD3 antibody [CD3-12] (AB11089)

Western blot analysis of ab11089 at 1 µg/mL.

All lanes:

Western blot - Anti-CD3 antibody [CD3-12] (ab11089) at 1 µg/mL

Lane 1:

Jurkat whole cell lysate at 20 µg

Lane 2:

Human thymus tissue lysate at 20 µg

Lane 3:

Mouse thymus tissue lysate at 20 µg

Lane 4:

Raji whole cell lysate at 20 µg

Lane 5:

Human brain tissue lysate at 20 µg

Lane 6:

Mouse brain tissue lysate at 20 µg

Secondary

Lanes 1 - 6:

Goat Anti-Rat IgG H&L (IRDye® 800CW) at 1/20000 dilution

Lanes 1 - 6:

Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed at 1/20000 dilution

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関連する標識済み抗体及び組成の異なる製品 (1)

  • 519 FITC

    FITC Anti-CD3 antibody [CD3-12]

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

CD3-12

アイソタイプ

IgG1

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

IHC-P, WB, Flow Cyt

applications

免疫原

Synthetic Peptide within Human CD3E. The exact immunogen used to generate this antibody is proprietary information.

P07766

特異性

IHC-P is not recommended in Human samples.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<p>Membrane permeabilization is recommended for this application. ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p>1mM EDTA pH8.0 is recommended for this purpose.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µg/mL", "FlowCyt-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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製品の詳細

Product Specifications

Anti-CD3 antibody [CD3-12] (ab11089) is a rat recombinant monoclonal antibody and is validated for use in in human, mouse, rat samples.
Anti-CD3 antibody [CD3-12] (ab11089) specifically detects CD3 (UniProt ID: P07766; Molecular weight: 21kDa) and is sold in 100 µg and 1 mg selling sizes.

Quality and Validation

Abcam's high quality manufacturing and validation processes ensure Anti-CD3 antibody [CD3-12] (ab11089) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-CD3 antibody [CD3-12] (ab11089) has been cited over 104 times in peer reviewed journals and is trusted by the scientific community.
Anti-CD3 antibody [CD3-12] (ab11089) has 18 independent reviews from customers.

Related Products

Conjugation-ready, carrier free format available for antibody clone CD3-12 - ab255972.
Antibody clone CD3-12 is also available pre-conjugated to a variety of labels for your convenience - FITC (ab34722).

Target Information

CD3, or Cluster of Differentiation 3, is a protein complex and T cell co-receptor that plays a crucial role in the activation of T cells, which are essential for the immune response. In oncology, CD3 is particularly significant due to its involvement in cancer immunotherapy. CD3 is targeted to enhance the body's ability to fight cancer. Bispecific antibodies (bsAbs) that target CD3 and a tumor antigen bring T cells into close proximity with cancer cells, promoting their destruction.

This product has switched from a hybridoma to recombinant production method on 1st November 2024.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed : 10384095, PubMed : 26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed : 38614099).
See full target information CD3E
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文献 (104)

Recent publications for all applications. Explore the full list and refine your search

The Journal of pharmacology and experimental therapeutics 388:12-22 PubMed37699708

2024

Blockade of Proteinase-Activated Receptor 2 (PAR2) Attenuates Neuroinflammation in Experimental Autoimmune Encephalomyelitis.

Applications

Unspecified application

Species

Unspecified reactive species

Rahil Eftekhari,Benjamin W Ewanchuk,Khalil S Rawji,Robin M Yates,Farshid Noorbakhsh,Hedwich F Kuipers,Morley D Hollenberg

NPJ precision oncology 7:135 PubMed38092866

2023

Imaging and AI based chromatin biomarkers for diagnosis and therapy evaluation from liquid biopsies.

Applications

Unspecified application

Species

Unspecified reactive species

Kiran Challa,Daniel Paysan,Dominic Leiser,Nadia Sauder,Damien C Weber,G V Shivashankar

Molecular neurobiology 61:3105-3120 PubMed37971656

2023

Beneficial Effect of Fingolimod in a Lafora Disease Mouse Model by Preventing Reactive Astrogliosis-Derived Neuroinflammation and Brain Infiltration of T-lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Rubio,Ángela Campos-Rodríguez,Pascual Sanz

Histology and histopathology 39:623-632 PubMed37916940

2023

mitigates autoimmune hepatitis by regulating IL-33-induced Treg/Th17 imbalance via the TLR2/4 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianguo Song,Juan Dai,Xueping Chen,Fei Ding,Yanbo Ding,Liang Ma,Liwen Zhang

Frontiers in immunology 14:1245708 PubMed37795103

2023

Novel miRNA-based drug CD5-2 reduces liver tumor growth in diethylnitrosamine-treated mice by normalizing tumor vasculature and altering immune infiltrate.

Applications

Unspecified application

Species

Unspecified reactive species

Ken Liu,Jinbiao Chen,Yang Zhao,Jade Boland,Ka Ka Ting,Glen Lockwood,Catriona McKenzie,James Kench,Mathew A Vadas,Jennifer R Gamble,Geoffrey W McCaughan

JID innovations : skin science from molecules to population health 3:100221 PubMed37731472

2023

Topical Calcipotriol Plus Imiquimod Immunotherapy for Nonkeratinocyte Skin Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Marjan Azin,Kenneth H Ngo,Jennet Hojanazarova,Shadmehr Demehri

JCI insight 8: PubMed37252795

2023

The MIF promoter SNP rs755622 is associated with immune activation in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tyler J Alban,Matthew M Grabowski,Balint Otvos,Defne Bayik,Wesley Wang,Ajay Zalavadia,Vlad Makarov,Katie Troike,Mary McGraw,Anja Rabljenovic,Adam Lauko,Chase Neumann,Gustavo Roversi,Kristin A Waite,Gino Cioffi,Nirav Patil,Thuy T Tran,Kathleen McCortney,Alicia Steffens,C Marcela Diaz,J Mark Brown,Kathleen M Egan,Craig M Horbinski,Jill S Barnholtz-Sloan,Prajwal Rajappa,Michael A Vogelbaum,Richard Bucala,Timothy A Chan,Manmeet S Ahluwalia,Justin D Lathia

JCI insight 8: PubMed37345654

2023

Perturbation of endoplasmic reticulum proteostasis triggers tissue injury in the thyroid gland.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohan Zhang,Crystal Young,Xiao-Hui Liao,Samuel Refetoff,Mauricio Torres,Yaron Tomer,Mihaela Stefan-Lifshitz,Hao Zhang,Dennis Larkin,Deyu Fang,Ling Qi,Peter Arvan

Nature communications 14:1213 PubMed36869026

2023

Fibroblastic reticular cells in lymph node potentiate white adipose tissue beiging through neuro-immune crosstalk in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lai Yee Cheong,Baile Wang,Qin Wang,Leigang Jin,Kelvin H M Kwok,Xiaoping Wu,Lingling Shu,Huige Lin,Sookja Kim Chung,Kenneth K Y Cheng,Ruby L C Hoo,Aimin Xu

EBioMedicine 89:104465 PubMed36796230

2023

Selective emergence of antibody-secreting cells in the multiple sclerosis brain.

Applications

Unspecified application

Species

Unspecified reactive species

Laurens Bogers,Hendrik J Engelenburg,Malou Janssen,Peter-Paul A Unger,Marie-José Melief,Annet F Wierenga-Wolf,Cheng-Chih Hsiao,Matthew R J Mason,Jörg Hamann,Jamie van Langelaar,Joost Smolders,Marvin M van Luijn
View all publications
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Abcam product promise

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