Anti-CD3 抗体 (ab16044)
Key features and details
- Rabbit polyclonal to CD3 epsilon
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-CD3 antibody
CD3 epsilon 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CD3 epsilon -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details
適用なし: IHC -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human CD3 epsilon aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab16206) -
ポジティブ･コントロール
- Recombinant Human CD3 epsilon protein (ab114153) can be used as a positive control in WB. This antibody gave a positive signal in Jurkat whole cells and thymus tissue from Mouse and Rat.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16044の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use a concentration of 5 µg/ml.
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| WB |
Use a concentration of 0.5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
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| 特記事項 |
|---|
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IP
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
ターゲット情報
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機能
The CD3 complex mediates signal transduction. -
配列類似性
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 916 Human
- Entrez Gene: 12501 Mouse
- Entrez Gene: 315609 Rat
- Omim: 186830 Human
- SwissProt: P07766 Human
- SwissProt: P22646 Mouse
- Unigene: 3003 Human
- Unigene: 210361 Mouse
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別名
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
画像
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Western blot - Anti-CD3 antibody (ab16044)All lanes : Anti-CD3 antibody (ab16044) at 1 µg/ml
Lane 1 : THP1 whole cell lysate (-ve control)
Lane 2 : Raji whole cell lysate (-ve control)
Lane 3 : Jurkat whole cell lysate
Lane 4 : Human Thymus tissue lysate
Lane 5 : Mouse Thymus tissue lysate
Lane 6 : Rat Thymus tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.Lanes 1 - 6: Merged signal (red and green). Green – ab16044 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab16044 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.
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Western blot - Anti-CD3 antibody (ab16044)All lanes : Anti-CD3 antibody (ab16044) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 2 : Thymus (Mouse) Tissue Lysate at 20 µg
Lane 3 : Thymus (Rat) Tissue Lysate at 20 µg
Secondary
All lanes : VHH Single Domain Anti-Rabbit IgG Fc (HRP) (ab191866) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Additional bands at: 18 kDa, 48 kDa, 62 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab16044 overnight at 4°C. Antibody binding was detected using VHH Single Domain Anti-Rabbit IgG Fc (HRP), and visualised using ECL development solution ab133406.
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Immunoprecipitation - Anti-CD3 antibody (ab16044)CD3 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to CD3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16044.
Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.
Band: 23kDa; CD3
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab16044 は 12 報の論文で使用されています。
- Hahn L et al. IL-13 as Target to Reduce Cholestasis and Dysbiosis in Abcb4 Knockout Mice. Cells 9:N/A (2020). PubMed: 32846954
- Tian L et al. Calotropin regulates the apoptosis of non-small cell cancer by regulating the cytotoxic T-lymphocyte associated antigen 4-mediated TGF-ß/ERK signaling pathway. Mol Med Rep 17:7683-7691 (2018). PubMed: 29620207
- Carbone ML et al. Topical Plant Polyphenols Prevent Type I Interferon Signaling in the Skin and Suppress Contact Hypersensitivity. Int J Mol Sci 19:N/A (2018). PubMed: 30200670
- Daniels BP et al. Regional astrocyte IFN signaling restricts pathogenesis during neurotropic viral infection. J Clin Invest 127:843-856 (2017). PubMed: 28134626
- Martin JL et al. Inhibition of basal-like breast cancer growth by FTY720 in combination with epidermal growth factor receptor kinase blockade. Breast Cancer Res 19:90 (2017). PubMed: 28778177