Anti-CD28 抗体 (ab245327)
Key features and details
- Rabbit polyclonal to CD28
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CD28 antibody
CD28 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CD28 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Cow
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免疫原
Synthetic peptide within Human CD28 aa 1-50. The exact sequence is proprietary. NP_006130.1
Database link: P10747 -
ポジティブ･コントロール
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: Jurkat whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245327 was affinity purified using an epitope specific to CD28 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245327の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 25 kDa.
|
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| IP |
Use at 2-10 µg/mg of lysate.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 25 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. -
組織特異性
Expressed in T-cells and plasma cells, but not in less mature B-cells. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 281050 Cow
- Entrez Gene: 940 Human
- Entrez Gene: 12487 Mouse
- Entrez Gene: 25660 Rat
- Omim: 186760 Human
- SwissProt: Q28071 Cow
- SwissProt: P10747 Human
- SwissProt: P31041 Mouse
see all -
別名
- CD 28 antibody
- CD28 antibody
- CD28 antigen antibody
see all
画像
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Immunoprecipitation - Anti-CD28 antibody (ab245327)
CD28 was immunoprecipitated from 1 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate using ab245327 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab245327 at 1 µg/ml.
Lane 1: ab245327 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in jurkat whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes. -
Western blot - Anti-CD28 antibody (ab245327)All lanes : Anti-CD28 antibody (ab245327) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 25 kDa
Exposure time: 3 minutes
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245327 は論文での使用が確認できていません。