JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB228178

Anti-CD276 抗体 [SP206], prediluted

Anti-CD276 antibody [SP206], prediluted

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal CD276 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

別名を表示する

CD276, B7H3, PSEC0249, UNQ309/PRO352, CD276 antigen, 4Ig-B7-H3, B7 homolog 3, Costimulatory molecule, B7-H3

12 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD276 with ab228178 in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178)

Formalin-fixed, paraffin-embedded human lung SCC tissue stained for CD276 with ab228178 in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD276 with ab228178 in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178)

Formalin-fixed, paraffin-embedded human lung tissue stained for CD276 with ab228178 in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178)

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for CD276 with ab228178 in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178)

Formalin-fixed, paraffin-embedded human colon tissue stained for CD276 with ab228178 in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178)

Formalin-fixed, paraffin-embedded human stomach tissue stained for CD276 with ab228178 in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD276 with ab228178 in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for CD276 with ab228178 in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178)

Formalin-fixed, paraffin-embedded human testis tissue stained for CD276 with ab228178 in immunohistochemical analysis.

Western blot - Anti-CD276 antibody [SP206], prediluted (AB228178)
  • WB

Lab

Western blot - Anti-CD276 antibody [SP206], prediluted (AB228178)

This data was developed using the same antibody clone in a different buffer formulation (ab227670).

Lanes 1- 4 : Merged signal (red and green). Green - ab227670 observed at 90-110 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab227670 was shown to react with CD276 antigen in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261756 (knockout cell lysate ab257098) was used. Wild-type HeLa and CD276 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab227670 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 400 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

Lanes 1 - 4:

Western blot - Anti-CD276 antibody [SP206], prediluted (ab228178) at 1/400 dilution

Lanes 1 - 4:

Western blot - Anti-CD276 antibody [SP206] (<a href='/products/primary-antibodies/cd276-antibody-sp206-ab227670'>ab227670</a>) at 1/400 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CD276 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CD276 knockout HeLa cell line (<a href='/products/cell-lines/human-cd276-knockout-hela-cell-line-ab261756'>ab261756</a>)

Lane 3:

LNCaP cell lysate at 20 µg

Lane 4:

Raji cell lysate at 20 µg

Predicted band size: 57 kDa

Observed band size: 90-110 kDa

false

Western blot - Anti-CD276 antibody [SP206], prediluted (AB228178)
  • WB

Lab

Western blot - Anti-CD276 antibody [SP206], prediluted (AB228178)

This data was developed using the same antibody clone in a different buffer formulation (ab227670).

Lanes 1-4 : Merged signal (red and green). Green - ab227670 observed at 90-110 kDa. Red - loading control ab8245 observed at 36 kDa.

ab227670 Anti-CD276 antibody [SP206] was shown to specifically react with CD276 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266658 (knockout cell lysate ab257097) was used. Wild-type and CD276 knockout samples were subjected to SDS-PAGE. ab227670 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

Lanes 1 - 4:

Western blot - Anti-CD276 antibody [SP206] (<a href='/products/primary-antibodies/cd276-antibody-sp206-ab227670'>ab227670</a>) at 1/1000 dilution

Lanes 1 - 4:

Western blot - Anti-CD276 antibody [SP206], prediluted (ab228178) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

CD276 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human CD276 knockout HEK-293T cell line (<a href='/products/cell-lines/human-cd276-knockout-hek-293t-cell-line-ab266658'>ab266658</a>)

Lane 3:

LNCaP cell lysate at 20 µg

Lane 4:

Raji cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 57 kDa

Observed band size: 90-110 kDa

false

関連する標識済み抗体及び組成の異なる製品 (10)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

SP206

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1", "IHCP-species-notes": "<p>Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.</p><p>Pre-diluted / ready to use.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A/G
精製に関する特記事項
Purified from TCS by protein A/G.
バッファー組成
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: Tris buffered saline, 1% BSA
出荷温度
Blue Ice
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD276 also known as B7-H3 is an immunoregulatory protein with a mass of approximately 57 kDa although post-translational modifications can increase its observed size. It is a member of the B7 family which is part of the immunoglobulin superfamily. CD276 is widely expressed in various tissues including placenta liver heart and also in many tumor cells. It is observed in lesser amounts in normal tissues making it an interesting target for cancer research. Various assays like HEK293 and HEK293T cell confluency studies often use CD276 to explore its expression under different conditions and seeding densities which are represented in confluency charts.
Biological function summary

CD276 modulates immune responses by acting as a co-inhibitory ligand impacting T-cell proliferation and cytokine synthesis. Not part of a known complex it plays a role in the adaptive immune system by providing negative feedback regulation that can inhibit the immune response. This capability has implications in preventing autoimmunity but may also contribute to tumor immune evasion by reducing effective anti-tumor responses. The B7-H3/CD276 interaction with receptors such as IL receptor family members suggests its diverse immunomodulatory functions.

Pathways

CD276 participates in the T-cell receptor (TCR) signaling pathways influencing immune checkpoint pathways. It interacts with other immune checkpoint proteins like PD-L1 and CTLA-4 but its exact receptor partner remains unidentified. The presence of CD276 in these pathways highlights its role in controlling T-cell activation and tolerance. By modulating TCR signals and impacting downstream effects CD276 serves as a regulatory point that can balance immune activation and inhibition critical in immune-mediated conditions.

CD276 has strong associations with cancer particularly in solid tumors such as prostate and breast cancer. It is often overexpressed in malignant tissues contributing to immune evasion by tumors. Researchers propose it as a potential target for cancer immunotherapy due to this attribute. CD276's link to disorders like asthma has also been suggested where its interaction with proteins involved in inflammatory pathways such as IL response elements can exacerbate the condition. This implicates it as a dual-role protein in both disease progression and potential therapeutic targeting.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
See full target information CD276
style
left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical pathology 69:307-12 PubMed26463756

2015

Differential diagnosis of bladder versus colorectal adenocarcinoma: keratin 7 and GATA3 positivity in nuclear ß-catenin-negative glandular tumours defines adenocarcinoma of the bladder.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Broede,Matthias Oll,Angela Maurer,Sabine Siegert,Stephan Stoerkel,Reinhard Golz,Kristina Schwamborn,Jürgen Veeck,Ruth Knuechel,Nadine T Gaisa
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB218280

Alexa Fluor® 488 Anti-CD276 antibody [EPNCIR122]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD276 antibody [EPNCIR122] (AB218280)

  • 0
  • 0
Primary Antibodies

AB218283

Alexa Fluor® 555 Anti-CD276 antibody [EPNCIR122]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-CD276 antibody [EPNCIR122] (AB218283)

  • 0
  • 0
Primary Antibodies

AB134161

Anti-CD276 antibody [EPNCIR122]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-CD276 antibody [EPNCIR122] (AB134161)

  • 0
  • 0
Primary Antibodies

AB219648

Anti-CD276 antibody [EPR20115]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [EPR20115] (AB219648)

  • 4
  • 1
Primary Antibodies

AB256585

Anti-CD276 antibody [EPNCIR122] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry - Anti-CD276 antibody [EPNCIR122] - BSA and Azide free (AB256585)

  • 0
  • 0
Primary Antibodies

AB227577

Anti-CD276 antibody [EPR20115] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [EPR20115] - BSA and Azide free (AB227577)

  • 0
  • 1
Primary Antibodies

AB209895

Anti-CD276 antibody [EPNCIR122] - Low endotoxin, Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry - Anti-CD276 antibody [EPNCIR122] - Low endotoxin, Azide free (AB209895)

  • 0
  • 0

組合せ製品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB205921

Anti-PD-L1 antibody [28-8]

BOND RX™ Validated|20ul selling size|Recombinant|KO Validated|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (AB205921)

  • 4
  • 15
Primary Antibodies

AB20034

Anti-FOXP3 antibody [236A/E7]

BOND RX™ Validated|Recombinant|Lab Essentials|Advanced Validation

Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] (AB20034)

  • 4
  • 38
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com