Anti-CD27 抗体 [LG.3A10] - Low endotoxin, Azide free (ab185776)
![Flow Cytometry - Anti-CD27 antibody [LG.3A10] - Low endotoxin, Azide free (ab185776)](https://www.abcam.co.jp/ps/products/185/ab185776/Images/ab185776-219641-anti-cd27-antibody-lg3a10-flow-cytometry.jpg "Flow Cytometry - Anti-CD27 antibody [LG.3A10] - Low endotoxin, Azide free (ab185776)")
Key features and details
- Armenian hamster monoclonal [LG.3A10] to CD27 - Low endotoxin, Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CD27 antibody [LG.3A10] - Low endotoxin, Azide free
CD27 一次抗体 製品一覧 -
製品の詳細
Armenian hamster monoclonal [LG.3A10] to CD27 - Low endotoxin, Azide free -
由来種
Armenian hamster -
特異性
When the monoclonal antibody LG.3A10 is crosslinked on purified T cells, proliferation is increased. In the absence of crosslinking, the antibody inhibits CD27-CD70 interaction. -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse, Human -
免疫原
The details of the immunogen for this antibody are not available.
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ポジティブ･コントロール
- BALB/c mouse splenocytes.
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特記事項
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by the LAL assay.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Constituent: 100% PBS
Aqueous buffer, no sodium azide. -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Functional grade purified. -
ポリ/モノ
モノクローナル -
クローン名
LG.3A10 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185776の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use 0.25µg for 105-8 cells.
In a final volume of 100 μL. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
Flow Cyt
Use 0.25µg for 105-8 cells. In a final volume of 100 μL. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. -
組織特異性
Found in most T-lymphocytes. -
配列類似性
Contains 3 TNFR-Cys repeats. -
翻訳後修飾
Phosphorylated and O-glycosylated. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 939 Human
- Entrez Gene: 21940 Mouse
- Omim: 186711 Human
- SwissProt: P26842 Human
- SwissProt: P41272 Mouse
- Unigene: 355307 Human
- Unigene: 367714 Mouse
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別名
- CD 27 antibody
- CD27 antibody
- CD27 antigen antibody
see all
画像
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Flow Cytometry - Anti-CD27 antibody [LG.3A10] - Low endotoxin, Azide free (ab185776)
Flow cytometric analysis of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC and 0.125 µg of ab185776 followed by Anti-Armenian Hamster IgG PE. Total viable cells were analyzed.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab185776 は論文での使用が確認できていません。
