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リコンビナントRabMAb

Anti-CD24 抗体 [EPR26528-17] (BSA and Azide free) (ab290745)

Submit a review Submit a question References (1)
Western blot - Anti-YTHDF1 antibody [EPR26528-17] - BSA and Azide free (ab290745)
  • Western blot - Anti-YTHDF1 antibody [EPR26528-17] - BSA and Azide free (ab290745)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [EPR26528-17] (BSA and Azide free) (ab290745)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [EPR26528-17] (BSA and Azide free) (ab290745)
  • Flow Cytometry - Anti-YTHDF1 antibody [EPR26528-17] - BSA and Azide free (ab290745)
  • Flow Cytometry - Anti-YTHDF1 antibody [EPR26528-17] - BSA and Azide free (ab290745)
  • Anti-YTHDF1 antibody [EPR26528-17] - BSA and Azide free (ab290745)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26528-17] to CD24 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-CD24 antibody [EPR26528-17] (BSA and Azide free)
    CD24 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26528-17] to CD24 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, Flow Cyt, ICC/IFmore details
    適用なし: IHC-Fr,IHC-P or IP

  • 種交差性

    交差種: Mouse
    非交差種: Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate, Neuro-2a (mouse neuroblastoma neuroblast) transfected with scrambled siRNA control, whole cell lysate. ICC/IF: Mouse PBMC (mouse primary peripheral blood mononuclear cell), Neuro-2a (mouse neuroblastoma neuroblast). Flow cyt.: Mouse PBMCs (mouse primary peripheral blood mononuclear cells), Neuro-2a (Mouse neuroblastoma neuroblast).

  • 特記事項

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab290745の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 61 kDa).
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 61 kDa).
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
追加情報
Is unsuitable for IHC-Fr,IHC-P or IP.

ターゲット情報

  • 機能

    Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.

  • 組織特異性

    B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.

  • 関連疾患

    Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface.

  • 配列類似性

    Belongs to the CD24 family.

  • 翻訳後修飾

    Extensively O-glycosylated.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD 24 antibody
      • CD24 antibody
      • CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody
      • CD24 antigen antibody
      • CD24 molecule antibody
      • CD24_HUMAN antibody
      • CD24A antibody
      • FLJ22950 antibody
      • FLJ43543 antibody
      • GPI linked surface mucin antibody
      • Heat stable antigen antibody
      • HSA antibody
      • MGC75043 antibody
      • Nectadrin antibody
      • Signal transducer CD24 antibody
      • Small cell lung carcinoma cluster 4 antigen antibody
      see all

    画像

    • Western blot - Anti-YTHDF1 antibody [EPR26528-17] - BSA and Azide free (ab290745)
      Western blot - Anti-YTHDF1 antibody [EPR26528-17] - BSA and Azide free (ab290745)
      Anti-CD24 antibody [EPR26528-17] (ab290730) at 1/1000 dilution + Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 61 kDa
      Observed band size: 37 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      This data was developed using ab290730, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 30044847; PMID: 8928617).

    • Western blot - Anti-YTHDF1 antibody [EPR26528-17] - BSA and Azide free (ab290745)
      Western blot - Anti-YTHDF1 antibody [EPR26528-17] - BSA and Azide free (ab290745)
      All lanes : Anti-CD24 antibody [EPR26528-17] (ab290730) at 1/1000 dilution

      Lane 1 : Neuro-2a (mouse neuroblastoma neuroblast) transfected with scrambled siRNA control, whole cell lysate
      Lane 2 : Neuro-2a transfected with siRNA specifically targeting CD24, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 61 kDa
      Observed band size: 37 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      This data was developed using ab290730, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

       

    • Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [EPR26528-17] (BSA and Azide free) (ab290745)
      Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [EPR26528-17] (BSA and Azide free) (ab290745)

      This data was developed using ab290730, the same antibody clone in a different buffer formulation. 

      Immunoflueorecent analysis of 4% paraformaldehyde fixed and 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) labeling CD24 with ab290730 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Green) at 1/1000 dilution. Confocal image showing cytoplasmic and membranous staining in the Neuro-2a cell line. Nuclear counterstain is DAPI (Blue). 

      Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594, Red) (ab195889) at 1/200 dilution was used as a counterstain. 

      Negative control: PBS was used instead of primary antibody followed by secondary antibody. 

    • Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [EPR26528-17] (BSA and Azide free) (ab290745)
      Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [EPR26528-17] (BSA and Azide free) (ab290745)

      This data was developed using ab290730, the same antibody clone in a different buffer formulation. 

      Immunoflueorecent analysis of 4% paraformaldehyde fixed and 0.1% TritonX-100 permeabilized mouse PBMC (mouse primary peripheral blood mononuclear cell) labelling CD24 with ab290730 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Green) at 1/1000 dilution. Confocal image showing cytoplasmic staining in subsets of mouse PBMCs. Nuclear counterstain is DAPI (Blue). 

      Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution was used as a counterstain. 

      Negative control: PBS was used instead of primary antibody folled by secondary antibody. 

    • Flow Cytometry - Anti-YTHDF1 antibody [EPR26528-17] - BSA and Azide free (ab290745)
      Flow Cytometry - Anti-YTHDF1 antibody [EPR26528-17] - BSA and Azide free (ab290745)

      This data was developed using ab290730, the same antibody clone in a different buffer formulation. 

      Flow cytometric analysis of mouse PBMCs (mouse primary peripheral blood mononuclear cells) labelling CD24 with ab290730 at 1/50 dilution (Right) compared with rabbit monoclonal IgG (ab172730) isotype control (Left). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. 

      Cells were stained with rabbit IgG or ab290730. Then stained with anti-CD3 conjugated to Alexa Fluor® 647.
      Gated on viable cells.

       

    • Flow Cytometry - Anti-YTHDF1 antibody [EPR26528-17] - BSA and Azide free (ab290745)
      Flow Cytometry - Anti-YTHDF1 antibody [EPR26528-17] - BSA and Azide free (ab290745)

      This data was developed using ab290730, the same antibody clone in a different buffer formulation. 

      Flow cytometric analysis of Neuro-2a (Mouse neuroblastoma neuroblast) labelling CD24 with ab290730 (Red) at 1/50 dilution compered with an isotype control rabbit monoclonal IgG (ab172730) (Black) an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as secondary antibody. Gated at viable cells. 

    • Anti-YTHDF1 antibody [EPR26528-17] - BSA and Azide free (ab290745)
      Anti-YTHDF1 antibody [EPR26528-17] - BSA and Azide free (ab290745)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab290745 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab290745 は 1 報の論文で使用されています。

    • Tsai KC  et al. The Blockade of Mitogen-Activated Protein Kinase 14 Activation by Marine Natural Product Crassolide Triggers ICD in Tumor Cells and Stimulates Anti-Tumor Immunity. Mar Drugs 21:N/A (2023). PubMed: 37103364

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