AB134110

Anti-CD239/BCAM 抗体 [EPR4165]

Name: Anti-CD239/BCAM 抗体 [EPR4165] (ab134110) | Abcam
Brand: Abcam Limited
SKU: AB134110
Price: 34000 JPY
Availability: InStock
Rating: 4 (6 reviews)

Anti-CD239/BCAM antibody [EPR4165]

20ul selling size
RabMAb
Recombinant
KO Validated
詳細を見る

(6 Reviews)

(3 Publications)

Rabbit Recombinant Monoclonal CD239/BCAM antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

別名を表示する

CD239, LU, MSK19, BCAM, Basal cell adhesion molecule, Auberger B antigen, B-CAM cell surface glycoprotein, F8/G253 antigen, Lutheran antigen, Lutheran blood group glycoprotein

4 Images

Lab

Immunocytochemistry - Anti-CD239/BCAM antibody [EPR4165] (AB134110)

ab134110 staining BCAM in A431 (positive) and A431-BCAM (negative) cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4 degrees with ab134110 at 0.2 ug/ml (shown in green) and ab7291, Anti-alpha Tubulin antibody [DM1A] - Loading Control (shown in pseudocolour magenta). Cells were then incubated with ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution and ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution for 1 hour at room temperature. Nuclear DNA was labeled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Revvity) and a maximum intensity projection of confocal sections is shown.

Immunocytochemistry/ Immunofluorescence - Anti-CD239/BCAM antibody [EPR4165] (AB134110)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD239/BCAM antibody [EPR4165] (AB134110)

Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) labeling CD239/BCAM with ab134110 at 1/100 dilution followed by ab150077 at 1/1000 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilied with 0.1% TritonX-100. DAPI was used as nuclear counter stain. Cells were counterstained wth ab195889 at 1/200 dilution. Confocal image showing mainly membranous staining in A431 cell line. Negative control : JurKat (PMID : 30078440) Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4165] (AB134110)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4165] (AB134110)

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling CD239/BCAM with ab134110 at 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-CD239/BCAM antibody [EPR4165] (AB134110)

Secondary antibody - goat anti-rabbit HRP (ab6721)

All lanes:

Western blot - Anti-CD239/BCAM antibody [EPR4165] (ab134110) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 67 kDa

Observed band size: 85 kDa

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Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4165

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IHC-P, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "Perform antigen retrieval before commencing with IHC staining protocol", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/2500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

出荷温度

Conditional Ambient

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD239 also known as basal cell adhesion molecule (BCAM) is an integral protein with a mass of about 60 kDa. This protein is primarily expressed in endothelial cells red blood cells and various epithelial tissues. BCAM plays a significant role in cell adhesion acting as a receptor for the extracellular matrix component laminin specifically laminin-511/521. It helps cells attach an essential role for maintaining tissue integrity and cellular communication.

Biological function summary

BCAM influences processes related to cell adhesion and motility. Although not explicitly part of a large complex it interacts closely with the cytoskeleton and mediators of signal transduction. Functions of BCAM involve linking extracellular matrix signals to the intracellular environment impacting cell movement and stabilization within tissues. It also modulates cellular responses to external mechanical stresses playing essential roles in maintaining normal cell function.

Pathways

BCAM is involved in several critical signaling processes including integrin-mediated adhesion and the MAPK/ERK pathway. How BCAM affects these pathways is through its interaction with integrins and elements related to cellular migration and proliferation. Within these pathways related proteins like laminins and other integrin subunits contribute to its adhesion properties and signaling functions enabling coordinated cellular responses to environmental cues.

BCAM is notably associated with sickle cell disease and certain cancers. In sickle cell disease the altered expression or function of BCAM on red blood cells causes abnormal adhesion to the endothelium leading to vaso-occlusion. This adhesion is often exacerbated by interactions with other proteins like L-selectin. In cancer BCAM overexpression correlates with increased tumor cell migration and invasion influencing metastasis and patient prognosis. These associations highlight BCAM's critical role in disease pathology and potential as a therapeutic target.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Transmembrane glycoprotein that functions as both a receptor and an adhesion molecule playing a crucial role in cell adhesion, motility, migration and invasion (PubMed : 9616226, PubMed : 31413112). Extracellular domain enables binding to extracellular matrix proteins, such as laminin, integrin and other ligands while its intracellular domain interacts with cytoskeletal proteins like hemoglobin, facilitating cell signal transduction (PubMed : 17158232). Serves as a receptor for laminin alpha-5/LAMA5 to promote cell adhesion (PubMed : 15975931). Mechanistically, JAK2 induces BCAM phosphorylation and activates its adhesion to laminin by stimulating a Rap1/AKT signaling pathway in the absence of EPOR (PubMed : 23160466).

See full target information BCAM

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 16:1216-1224 PubMed30116372

2018

Application of a novel targeting nanoparticle contrast agent combined with magnetic resonance imaging in the diagnosis of intraductal papillary mucinous neoplasm.

Applications

Unspecified application

Species

Unspecified reactive species

Min Sun,Liqing Kang,Yanchao Cui,Guoce Li

Experimental cell research 323:7-27 PubMed24569142

2014

Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments.

Applications

Flow Cyt

Species

Mouse

Leslee Sprague,Maria Muccioli,Michelle Pate,Manindra Singh,Chengkai Xiong,Alexander Ostermann,Brandon Niese,Yihan Li,Yandi Li,Maria Cecilia Courreges,Fabian Benencia

Glycobiology 23:1240-9 PubMed23918816

2013

Systemic alteration of cell-surface and secreted glycoprotein expression in malignant breast cancer cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Leslie C Timpe,Roger Yen,Nicole V Haste,Christina Litsakos-Cheung,Ten-Yang Yen,Bruce A Macher

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD239/BCAM 抗体 [EPR4165]

Anti-CD239/BCAM antibody [EPR4165]

Immunocytochemistry - Anti-CD239/BCAM antibody [EPR4165] (AB134110)

Immunocytochemistry/ Immunofluorescence - Anti-CD239/BCAM antibody [EPR4165] (AB134110)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD239/BCAM antibody [EPR4165] (AB134110)

Western blot - Anti-CD239/BCAM antibody [EPR4165] (AB134110)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

Abcam product promise