Anti-CD23 抗体 [SP23] (ab16702)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP23] to CD23
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD23 antibody [SP23]
CD23 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP23] to CD23 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment within Human CD23 aa 1-250. The exact sequence is proprietary.
Database link: P06734 -
ポジティブ・コントロール
- IHC-P: Human tonsil. Flow Cyt: Jurkat cell lysate. WB: Raji cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
SP23 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16702の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration.
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IHC-P |
1/100.
Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
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Flow Cyt |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
Use at an assay dependent concentration. |
IHC-P
1/100. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
Flow Cyt
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). -
配列類似性
Contains 1 C-type lectin domain. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Cell membrane. Secreted. Also exists as a soluble excreted form. - Information by UniProt
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参照データベース
- Entrez Gene: 2208 Human
- Omim: 151445 Human
- SwissProt: P06734 Human
- Unigene: 465778 Human
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別名
- Blast 2 antibody
- BLAST-2 antibody
- Blast2 antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded Human tonsil labeling CD23 with ab16702 at 1/100 (76 µg/ml). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16702 for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Counterstained with Hematoxylin and mounted with DPX.
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This image was generated using a previous batch manufactured using hybridoma production method.
Flow cytometric analysis of rabbit anti-CD23 (SP23) antibody ab16702 (1/100) in Jurkats (green) compared to negative control of rabbit IgG (blue).
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Anti-CD23 antibody [SP23] (ab16702) at 1/25 dilution + lysate prepared from Raji cells
Observed band size: 45 kDa why is the actual band size different from the predicted?This image was generated using a previous batch manufactured using hybridoma production method.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab16702 は 5 報の論文で使用されています。
- Hayashi Y et al. Density and maturity of peritumoral tertiary lymphoid structures in oesophageal squamous cell carcinoma predicts patient survival and response to immune checkpoint inhibitors. Br J Cancer 128:2175-2185 (2023). PubMed: 37016103
- Xu W et al. Heterogeneity in tertiary lymphoid structures predicts distinct prognosis and immune microenvironment characterizations of clear cell renal cell carcinoma. J Immunother Cancer 11:N/A (2023). PubMed: 38040418
- Fukano C et al. Establishment of enzyme-linked immunosorbent facilitated antigen binding as a biomarker assay for Japanese cedar pollen allergy immunotherapy. J Pharmacol Sci 140:223-227 (2019). PubMed: 31353210
- Siliņa K et al. A Quantitative Pathology Approach to Analyze the Development of Human Cancer-Associated Tertiary Lymphoid Structures. Methods Mol Biol 1845:71-86 (2018). PubMed: 30141008
- Koletsa T et al. Synchronous Presence of Nasopharyngeal Carcinoma and Marginal Zone (MALT-Type) B-Cell Lymphoma in the Pharynx. Patholog Res Int 2011:340763 (2011). PubMed: 21660262