Anti-CD23 抗体 [EPR26479-9] (BSA and Azide free) (ab302527)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR26479-9] (BSA and Azide free) (AB302527)](https://www.abcam.co.jp/ps/products/302/ab302527/Images/AB302527-2-anti-cd23-antibody-epr264799-immunohistochemistry-mouse-lymph-nodes1.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR26479-9] (BSA and Azide free) (AB302527)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26479-9] to CD23 - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-CD23 antibody [EPR26479-9] (BSA and Azide free)
CD23 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR26479-9] to CD23 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details
適用なし: Flow Cyt,ICC/IF,IHC-Fr,IP or WB -
種交差性
交差種: Mouse
非交差種: Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- IHC-P: Mouse spleen, lymph nodes FFPE tissue section.
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特記事項
ab302527 is the carrier-free version of ab302526.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.20
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR26479-9 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab302527の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). -
配列類似性
Contains 1 C-type lectin domain. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Cell membrane. Secreted. Also exists as a soluble excreted form. - Information by UniProt
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参照データベース
- Entrez Gene: 14128 Mouse
- SwissProt: P20693 Mouse
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別名
- Blast 2 antibody
- BLAST-2 antibody
- Blast2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR26479-9] (BSA and Azide free) (AB302527)
This data was developed using ab302526, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse lymph nodes tissue labeling CD23 with ab302526 at 1/100 dilution (4.67 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) as secondary antibody. Positive staining on mouse lymph nodes. The section was incubated with ab302526 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody without primary.
Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR26479-9] (BSA and Azide free) (AB302527)](https://www.abcam.co.jp/ps/products/302/ab302527/Images/AB302527-3-anti-cd23-antibody-epr264799-immunohistochemistry-mouse-spleen1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR26479-9] (BSA and Azide free) (AB302527)This data was developed using ab302526, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD23 with ab302526 at 1/100 dilution (4.67 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) as secondary antibody. Positive staining on mouse spleen. The section was incubated with ab302526 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody without primary.
Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR26479-9] (BSA and Azide free) (AB302527)](https://www.abcam.co.jp/ps/products/302/ab302527/Images/AB302527-4-anti-cd23-antibody-epr264799-immunohistochemistry-mouse-cardiac-muscle1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR26479-9] (BSA and Azide free) (AB302527)This data was developed using ab302526, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue to label CD23 with ab302526 at 1/100 dilution (4.67 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Negative control: no staining on mouse cardiac muscle. The section was incubated with ab302526 at 4°C overnight. Counterstained with Hematoxylin.
Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)
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![Anti-CD23 antibody [EPR26479-9] (BSA and Azide free) (AB302527)](https://www.abcam.co.jp/ps/products/302/ab302527/Images/AB302527-5-Benefits-of-Recombinant-antibodies.jpg)
Anti-CD23 antibody [EPR26479-9] (BSA and Azide free) (AB302527)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab302527 は論文での使用が確認できていません。
