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Anti-CD226 (phospho S329) 抗体 (ab61790)

Reviews (1)Q&A (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 (phospho S329) antibody (ab61790)
  • Western blot - Anti-CD226 (phospho S329) antibody (ab61790)

Key features and details

  • Rabbit polyclonal to CD226 (phospho S329)
  • Suitable for: WB, IHC-P
  • Reacts with: Human, African green monkey
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-CD226 (phospho S329) antibody
    CD226 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to CD226 (phospho S329)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human, African green monkey
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide corresponding to Human CD226 aa 250-350 (phospho S329).
    Database link: Q15762

  • ポジティブ･コントロール

    • Human brain tissue; extracts from COS7 cells

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab61790の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 39 kDa).
IHC-P
1/50 - 1/100.
特記事項
WB
1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 39 kDa).
IHC-P
1/50 - 1/100.

ターゲット情報

  • 機能

    Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell.

  • 組織特異性

    Expressed by peripheral blood T-lymphocytes.

  • 配列類似性

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • adhesion glycoprotein antibody
      • CD226 antibody
      • CD226 antigen antibody
      • CD226 molecule antibody
      • CD226_HUMAN antibody
      • DNAM 1 antibody
      • DNAM-1 antibody
      • DNAM1 antibody
      • DNAX accessory molecule 1 antibody
      • platelet and T cell activation antigen 1 antibody
      • PTA1 antibody
      • T lineage specific activation antigen 1 antigen antibody
      • TLiSA1 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 (phospho S329) antibody (ab61790)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD226 (phospho S329) antibody (ab61790)
      Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab61790 at 1/50-1/100 dilution with (+) or without (-) immunising phosphopeptide.
    • Western blot - Anti-CD226 (phospho S329) antibody (ab61790)
      Western blot - Anti-CD226 (phospho S329) antibody (ab61790)
      All lanes : Anti-CD226 (phospho S329) antibody (ab61790) at 1/500 dilution

      Lane 1 : Extracts from COS7 cells
      Lane 2 : Extracts from COS7 cells with the immunising phosphopeptide

      Predicted band size: 39 kDa
      Observed band size: ~ 59 kDa why is the actual band size different from the predicted?

    参考文献 (0)

    ab61790 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab61790 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Flow Cytometry abreview for Anti-CD226 (phospho S329) antibody

     Excellent
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Jurkat)
    Permeabilization
    Yes - 50% Methanol
    Gating Strategy
    FSC-A v SSC-A (cell gate) followed by single cells gate (SSC-A v SSC-H)
    Specification
    Jurkat
    Fixation
    eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Diana Shinko

    Verified customer

    投稿 Jun 29 2022

    Question

    je voulais savoir si l'anticorps Anti-CD226 (phospho S329) antibody (ab61790) avait été testé et fonctionnait sur des coupes congelées.

    Read More
    Answer

    Merci de votre intérêt pour ab61790.

    Cet anticorps n’a pas encore été testé en IHC-Fr et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires.
    Cependant, si vous souhaitez tester ab61790 en IHC-Fr, il me sera possible de vous offrir une remise.
    Après soumission d’une Abreview avec vos résultats, un anticorps primaire de votre choix vous sera offert.

    Etapes à suivre :

    1. Nous confirmer que vous souhaitez tester ab61790 en IHC-Fr afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de ab61790

    2. Acheter ab61790 par téléphone, fax ou internet (https://www.abcam.com)

    3. Le tester en IHC-Fr

    4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews

    5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire.
    Même si ab61790 ne pas fonctionne pas en IHC-Fr, après soumission de votre Abreview, vous recevrez quand même gratuitement un anticorps primaire de votre choix.

    N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle.

    Termes et Conditions : https://www.abcam.com/collaborationdiscount.

    Read More

    Question

    Dear colleague,
    I would like to know if the Anti-CD226 (phospho S329) antibody (ab61790) has been tested in frozen tissu sections (for immunohistochemistry)
    Best regards,

    Read More
    Answer

    Thank you for contacting us and for your interest in our products.

    As far as we are aware, ab61790 has never been tested in frozen sections (IHC-Fr). All tested and guaranteed applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

    If you would like to test the antibody in this untested application, please contact our Scientific Support team prior to the purchase by replying to this message as you may be eligible for our testing discount program.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

    Read More

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