Anti-CD22 (phospho Y842) 抗体 [Y507] (ab32355)
![Western blot - Anti-CD22 (phospho Y842) antibody [Y507] (ab32355)](https://www.abcam.co.jp/ps/products/32/ab32355/Images/ab32355-44534-anti-cd22-phospho-y842-antibody-y507-western-blot.jpg "Western blot - Anti-CD22 (phospho Y842) antibody [Y507] (ab32355)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y507] to CD22 (phospho Y842)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD22 (phospho Y842) antibody [Y507]
CD22 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [Y507] to CD22 (phospho Y842) -
由来種
Rabbit -
特異性
The antibody detects CD22 phosphorylation on Tyrosine 842 and is predicted to detect splice isoforms 2 based on sequence homology. -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,IHC or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
Y507 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32355の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000. Detects a band of approximately 150 kDa (predicted molecular weight: 95 kDa).
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| 特記事項 |
|---|
|
WB
1/2000. Detects a band of approximately 150 kDa (predicted molecular weight: 95 kDa). |
追加情報
Is unsuitable for Flow Cyt,IHC or IP.
ターゲット情報
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機能
Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. -
組織特異性
B-lymphocytes. -
配列類似性
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
ドメイン
Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
翻訳後修飾
Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
Phosphorylated on tyrosine residues by LYN. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 933 Human
- Omim: 107266 Human
- SwissProt: P20273 Human
- Unigene: 579691 Human
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別名
- B cell receptor CD22 precursor antibody
- B lymphocyte cell adhesion molecule antibody
- B-cell receptor CD22 antibody
see all
画像
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Western blot - Anti-CD22 (phospho Y842) antibody [Y507] (ab32355)All lanes : Anti-CD22 (phospho Y842) antibody [Y507] (ab32355) at 1/2000 dilution
Lane 1 : Raji Cell Lysate, Untreated
Lane 2 : Raji Cell Lysate, Treated with Pervanadate
Lysates/proteins at 10 µg per lane.
Predicted band size: 95 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
![Anti-CD22 (phospho Y842) antibody [Y507] (ab32355)](https://www.abcam.co.jp/ps/products/32/ab32355/Images/ab32355-2-benefits-of-recombinant-antibodies.png)
Anti-CD22 (phospho Y842) antibody [Y507] (ab32355)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab32355 は 1 報の論文で使用されています。
- Hsia CW et al. Suppression of Human Platelet Activation via Integrin aIIbß3 Outside-In Independent Signal and Reduction of the Mortality in Pulmonary Thrombosis by Auraptene. Int J Mol Sci 20:N/A (2019). PubMed: 31717348