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AB32123

Anti-CD22 (phospho Y822) 抗体 [Y506]

Anti-CD22 (phospho Y822) antibody [Y506]

3

(1 Review)

|

(2 Publications)

Rabbit Monoclonal CD22 phospho Y822 antibody. Suitable for Flow Cyt, Dot, WB and reacts with Human, Synthetic peptide samples. Cited in 2 publications.

別名を表示する

CD22, SIGLEC2, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, Sialic acid-binding Ig-like lectin 2, T-cell surface antigen Leu-14, BL-CAM, Siglec-2

4 Images
Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)
  • WB

Unknown

Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

All lanes:

Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123) at 1/1000 dilution

Lane 1:

Untreated Raji cell lysate

Lane 2:

Raji cell lysate treated with Pervanadate

Predicted band size: 95 kDa

Observed band size: 150 kDa

false

Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)
  • WB

Unknown

Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123) at 1/1000 dilution

Lane 1:

Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 10 µg

Lane 2:

Ramos treated with 1mM pervanadate for 30 min whole cell lysate at 10 µg

Lane 3:

Ramos treated with 1mM pervanadate for 30 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase. at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 95 kDa

Observed band size: 150 kDa

false

Flow Cytometry - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

Flow cytometric analysis of permeabilized K562 cells, untreated (green) or Pervanadate treated (red) using ab32123

Dot Blot - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)
  • Dot

Unknown

Dot Blot - Anti-CD22 (phospho Y822) antibody [Y506] (AB32123)

Dot blot analysis of CD22 (phospho Y822) phospho peptide (Lane 1) and CD22 non-phospho peptide (Lane 2) labelling CD22 (phospho Y822) with ab32123 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer : 5% NFDM/TBST. Dilution buffer : 5% NFDM /TBST.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-CD22 (phospho Y822) antibody [Y506] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

Y506

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, Flow Cyt, Dot

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

ab32123 recognises CD22 phosphorylation on Tyrosine 822. This antibody is predicted to detect splice isoform 2 based on sequence homology.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/10", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD22 also known as Siglec-2 or B-lymphocyte cell adhesion molecule (BL-CAM) is a transmembrane protein with a mass of around 140 kDa. It is selectively expressed on the surface of mature B cells and some immature B cells. This protein plays an important role in the immune system particularly in the regulation of B cell function. CD22 functions by modulating signal transduction processes through its cytoplasmic domain which contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs).
Biological function summary

CD22 acts mainly as a negative regulator of B cell receptor (BCR) signaling. It is part of a complex that interacts with the BCR and other accessory molecules. CD22 helps maintain B cell tolerance by inhibiting overactive signaling and preventing autoimmune responses. By binding to sialic acid-containing glycans CD22 modulates interactions between B cells and other cells aiding in the maintenance of immune homeostasis.

Pathways

CD22 plays a critical role in the BCR signaling pathway and the regulation of phosphatidylinositol 3-kinase (PI3K) signaling. It works together with proteins like CD19 and SHP-1 that are key components of these pathways. By influencing these signaling cascades CD22 contributes to the modulation of B cell activation proliferation and apoptosis ensuring proper functioning of the immune response.

CD22 is associated with autoimmune conditions and hematological malignancies such as hairy cell leukemia (HCL). Therapeutic approaches targeting CD22 such as anti-CD22 antibodies are being explored for treating these diseases. CD22's role in these conditions is connected with proteins involved in the autoimmune pathways or cancer development offering potential therapeutic targets for intervention.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Most highly expressed siglec (sialic acid-binding immunoglobulin-like lectin) on B-cells that plays a role in various aspects of B-cell biology including differentiation, antigen presentation, and trafficking to bone marrow (PubMed : 34330755, PubMed : 8627166). Binds to alpha 2,6-linked sialic acid residues of surface molecules such as CD22 itself, CD45 and IgM in a cis configuration. Can also bind to ligands on other cells as an adhesion molecule in a trans configuration (PubMed : 20172905). Acts as an inhibitory coreceptor on the surface of B-cells and inhibits B-cell receptor induced signaling, characterized by inhibition of the calcium mobilization and cellular activation. Mechanistically, the immunoreceptor tyrosine-based inhibitory motif domain is phosphorylated by the Src kinase LYN, which in turn leads to the recruitment of the protein tyrosine phosphatase 1/PTPN6, leading to the negative regulation of BCR signaling (PubMed : 8627166). If this negative signaling from is of sufficient strength, apoptosis of the B-cell can be induced (PubMed : 20516366).
See full target information CD22 phospho Y822
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of 112:E6515-24 PubMed26561584

2015

An extracatalytic function of CD45 in B cells is mediated by CD22.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Coughlin,Mark Noviski,James L Mueller,Ammarina Chuwonpad,William C Raschke,Arthur Weiss,Julie Zikherman

Blood 116:1698-704 PubMed20516366

2010

IVIg modulates BCR signaling through CD22 and promotes apoptosis in mature human B lymphocytes.

Applications

Flow Cyt

Species

Human

Jean-François Séïté,Divi Cornec,Yves Renaudineau,Pierre Youinou,Rizgar A Mageed,Sophie Hillion
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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