AB240122

Anti-CD204 抗体 [EPR7536] - BSA and Azide free

Anti-CD204 antibody [EPR7536] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal CD204 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

別名を表示する

CD204, SCARA1, MSR1, Macrophage scavenger receptor types I and II, Macrophage acetylated LDL receptor I and II, Scavenger receptor class A member 1

4 Images

Unknown

Western blot - Anti-CD204 antibody [EPR7536] - BSA and Azide free (AB240122)

This data was developed using ab151707, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CD204 antibody [EPR7536] (<a href='/products/primary-antibodies/cd204-antibody-epr7536-ab151707'>ab151707</a>) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 10 µg

Lane 2:

THP1 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 50 kDa,79 kDa

false

Lab

Western blot - Anti-CD204 antibody [EPR7536] - BSA and Azide free (AB240122)

This data was developed using ab151707, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD204 antibody [EPR7536] (<a href='/products/primary-antibodies/cd204-antibody-epr7536-ab151707'>ab151707</a>) at 1/1000 dilution

Lane 1:

THP-1 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 75 kDa

false

Lab

Western blot - Anti-CD204 antibody [EPR7536] - BSA and Azide free (AB240122)

This data was developed using ab151707, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD204 antibody [EPR7536] (<a href='/products/primary-antibodies/cd204-antibody-epr7536-ab151707'>ab151707</a>) at 1/1000 dilution

All lanes:

Mouse placenta tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 75 kDa

false

Lab

Western blot - Anti-CD204 antibody [EPR7536] - BSA and Azide free (AB240122)

This data was developed using ab151707, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD204 antibody [EPR7536] (<a href='/products/primary-antibodies/cd204-antibody-epr7536-ab151707'>ab151707</a>) at 1/5000 dilution

All lanes:

Rat placenta tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 75 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR7536

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse, Rat, Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

ab240122 is the carrier-free version of ab151707.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD204 also known as MSR1 or the macrophage scavenger receptor 1 is a class A scavenger receptor. It has a molecular mass of approximately 80-85 kDa. CD204 is mainly expressed on the surface of macrophages as well as some dendritic cells. This receptor plays a role in mediating the endocytosis of various polyanionic molecules such as modified low-density lipoproteins. CD204 serves as a recognition site for apoptotic cells signaling molecules and some types of bacteria.

Biological function summary

CD204 influences inflammatory responses and lipid metabolism. It does not function alone but as part of a complex involving several other molecules like pattern recognition receptors. CD204 recognizes and binds to modified lipoproteins and is significant in processes like phagocytosis and clearance of cellular debris. Its activity affects immune response modulation and can influence atherosclerosis development by its interaction with oxidized low-density lipoproteins.

Pathways

The role of CD204 extends to key cellular and immunological pathways. This receptor is involved in the Toll-like receptor signaling pathway which is important for innate immunity besides participating in the macrophage activation pathways. In these pathways CD204 interacts with other proteins including CD36 and SRA. This interaction helps coordinate inflammatory responses and the resolution of inflammation.

CD204 is associated with atherosclerosis and chronic inflammation. Its connection with these conditions arises from its role in lipid uptake and immune cell regulation within the vascular system. In the context of atherosclerosis CD204's interaction with Apolipoprotein E (ApoE) influences the formation of foam cells which are significant in the disease's progression. Similarly in chronic inflammation CD204 modulates macrophage behavior impacting the inflammatory environment and potentially affecting the disease outcome.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL) (PubMed : 2251254). Isoform III does not internalize acetylated LDL (PubMed : 9548586).

See full target information MSR1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 13:5323-5335 PubMed32606750

2020

Connexin 43 Modulates the Cellular Resistance to Paclitaxel via Targeting β-Tubulin in Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Fu,Xiaoyin Sun,Zhangyuan Gu,Zhigang Zhuang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD204 抗体 [EPR7536] - BSA and Azide free

Anti-CD204 antibody [EPR7536] - BSA and Azide free

Western blot - Anti-CD204 antibody [EPR7536] - BSA and Azide free (AB240122)

Western blot - Anti-CD204 antibody [EPR7536] - BSA and Azide free (AB240122)

Western blot - Anti-CD204 antibody [EPR7536] - BSA and Azide free (AB240122)

Western blot - Anti-CD204 antibody [EPR7536] - BSA and Azide free (AB240122)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise