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AB272017

Anti-CD2 抗体 [SP304] - BSA and Azide free

Anti-CD2 antibody [SP304] - BSA and Azide free

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Rabbit Recombinant Monoclonal CD2 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.

別名を表示する

CD2, SRBC, T-cell surface antigen CD2, Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, T-cell surface antigen T11/Leu-5

12 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)

Formalin-fixed, paraffin-embedded human stomach tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)

Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)

Formalin-fixed, paraffin-embedded human colon tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)

Formalin-fixed, paraffin-embedded human uterus tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)

Formalin-fixed, paraffin-embedded human thymus tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] - BSA and Azide free (AB272017)

Immunohistochemistry analysis of paraffin-embedded human tonsil tissue sections labelling CD2 with ab227698 at 1/100 dilution. The section was incubated with ab227698 for 10 mins at room temperature. LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining on the human tonsil tissue, performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227698).

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

SP304

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

AB119993

Anti-CD2 antibody [EPR3693]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-CD2 antibody [EPR3693] (AB119993)

  • 0
  • 0
Primary Antibodies

AB131276

Anti-CD2 antibody [EPR6451]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD2 antibody [EPR6451] (AB131276)

  • 0
  • 0
Primary Antibodies

AB219411

Anti-CD2 antibody [EPR21825]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR21825] (AB219411)

  • 0
  • 0
Primary Antibodies

AB247951

Anti-CD2 antibody [EPR3693] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-CD2 antibody [EPR3693] - BSA and Azide free (AB247951)

  • 0
  • 0
Primary Antibodies

AB248400

Anti-CD2 antibody [EPR6451] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD2 antibody [EPR6451] - BSA and Azide free (AB248400)

  • 0
  • 0
Primary Antibodies

AB231059

Anti-CD2 antibody [EPR21825] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR21825] - BSA and Azide free (AB231059)

  • 0
  • 0

組合せ製品

Sample Prep & Detection Kits

AB269821

Alexa Fluor® 568 Conjugation Kit (Fast) - Lightning-Link®

Conjugation - Alexa Fluor® 568 Conjugation Kit (Fast) - Lightning-Link® (AB269821)

  • 0
  • 0
Sample Prep & Detection Kits

AB188290

Gold-Maleimide Conjugation Kit (40nm, 20 OD)

Lateral flow assay - Gold-Maleimide Conjugation Kit (40nm, 20 OD) (AB188290)

  • 0
  • 0
Primary Antibodies

AB243903

Anti-TIGIT antibody [BLR047F] - BSA free

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [BLR047F] - BSA free (AB243903)

  • 4
  • 7
Primary Antibodies

AB246518

Anti-CXCL13 antibody [EPR23400-92]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody [EPR23400-92] (AB246518)

  • 5
  • 1
Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

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  • 80
Primary Antibodies

AB131254

Anti-CD27 antibody [EPR8569]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-CD27 antibody [EPR8569] (AB131254)

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.Incubate with primary antibody for 10 minutes at room temperature.</p>" } } }
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製品の詳細

ab272017 is the carrier-free version of ab227698.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A/G
精製に関する特記事項
Purified from TCS by protein A/G.
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD2 also known as T11 or LFA-2 acts as a cell surface glycoprotein expressed mainly on T-cells natural killer cells and thymocytes. This protein has a molecular mass of approximately 50-55 kDa. CD2 plays a critical role in facilitating cell-cell adhesion and signal transduction. It serves as a receptor for the CD58 ligand on antigen-presenting cells supporting the interaction between T-cells and these cells. By enhancing adhesion CD2 contributes to the formation of the immunological synapse necessary for effective immune response.
Biological function summary

The functioning of CD2 helps in the modulation of immune responses and T-cell activation. CD2 participates in signal transduction leading to cytokine production and proliferation. It is associated with the immunological synapse complex working closely with other surface molecules like Lck and CD3 to promote T-cell responses. This collaboration is vital for the development and function of immune cells driving adaptive immunity.

Pathways

CD2 engages in pathways integral to T-cell activation and signal transduction. A well-known pathway involving CD2 is the TCR (T-cell Receptor) signaling pathway where it partners with CD3 to initiate T-cell activation. Additionally CD2 influences the LAT (Linker for Activation of T-cells) pathway contributing to downstream signaling cascades that involve various signaling proteins such as Lck and ZAP-70 ensuring proper T-cell function.

CD2 shows connections to autoimmune diseases and cancers. For instance its involvement in abnormal T-cell activation links CD2 to autoimmune conditions like rheumatoid arthritis. Also various leukemias demonstrate altered CD2 expression suggesting a role in cancer pathogenesis. Through these disease states CD2's interaction with the CD58 protein becomes critical as this interaction affects the activity of T-cells in the disease milieu influencing disease progression and treatment outcomes.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
See full target information CD2
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Abcam product promise

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