AB212988

Anti-CD2 抗体 [1E7E8.G4] - BSA and Azide free

Name: Anti-CD2 抗体 [1E7E8.G4] - BSA and Azide free (ab212988) | Abcam
Brand: Abcam Limited
SKU: AB212988
Price: 129000 JPY
Availability: InStock

Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free

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Mouse Monoclonal CD2 antibody. Carrier free. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD2 protein.

別名を表示する

CD2, SRBC, T-cell surface antigen CD2, Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, T-cell surface antigen T11/Leu-5

3 Images

Flow Cytometry - Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free (AB212988)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free (AB212988)

Flow Cytometric Analysis of MOLT4 cells using ab193344 followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193344).

Immunocytochemistry/ Immunofluorescence - Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free (AB212988)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free (AB212988)

Immunofluorescence staining of MOLT-4 cells using ab193344 followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193344).

Supplier Data

Western blot - Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free (AB212988)

All lanes:

Western blot - Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free (ab212988) at 2 µg/mL

All lanes:

Jurkat cell lysate

Predicted band size: 39 kDa

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Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

1E7E8.G4

アイソタイプ

IgG2a

軽鎖のタイプ

kappa

キャリアフリー

Yes

交差種

Human

アプリケーション

WB, Flow Cyt, ICC/IF

applications

免疫原

Cell preparation containing CD2 protein. The exact immunogen used to generate this antibody is proprietary information.

P06729

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µL for 10^6 Cells", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "" } } }

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製品の詳細

ab212988 is a carrier free version of ab193344.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD2 also known as T11 or LFA-2 acts as a cell surface glycoprotein expressed mainly on T-cells natural killer cells and thymocytes. This protein has a molecular mass of approximately 50-55 kDa. CD2 plays a critical role in facilitating cell-cell adhesion and signal transduction. It serves as a receptor for the CD58 ligand on antigen-presenting cells supporting the interaction between T-cells and these cells. By enhancing adhesion CD2 contributes to the formation of the immunological synapse necessary for effective immune response.

Biological function summary

The functioning of CD2 helps in the modulation of immune responses and T-cell activation. CD2 participates in signal transduction leading to cytokine production and proliferation. It is associated with the immunological synapse complex working closely with other surface molecules like Lck and CD3 to promote T-cell responses. This collaboration is vital for the development and function of immune cells driving adaptive immunity.

Pathways

CD2 engages in pathways integral to T-cell activation and signal transduction. A well-known pathway involving CD2 is the TCR (T-cell Receptor) signaling pathway where it partners with CD3 to initiate T-cell activation. Additionally CD2 influences the LAT (Linker for Activation of T-cells) pathway contributing to downstream signaling cascades that involve various signaling proteins such as Lck and ZAP-70 ensuring proper T-cell function.

CD2 shows connections to autoimmune diseases and cancers. For instance its involvement in abnormal T-cell activation links CD2 to autoimmune conditions like rheumatoid arthritis. Also various leukemias demonstrate altered CD2 expression suggesting a role in cancer pathogenesis. Through these disease states CD2's interaction with the CD58 protein becomes critical as this interaction affects the activity of T-cells in the disease milieu influencing disease progression and treatment outcomes.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

See full target information CD2

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