Anti-CD19 抗体 [SP291] - C-terminal

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD19 with ab227688 at 1/100 (0.15 ug/ml) dilution, followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining in human tonsil.
The section was incubated with ab227688 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

This data was developed using ab237772, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human spleen tissue labeling CD8 alpha with ab245118 at 1/500 dilution, CD4 with ab238798 at 1/500, and CD19 with ab237772 at 1/5000 dilution.

Panel A : merged staining of anti-CD8 alpha (magenta; Opal™690), anti-CD4 (green; Opal™520) and anti-CD19 (red; Opal™570) on human spleen.
Panel B : anti-CD8 alpha stained on cytotoxic T cells.
Panel C : anti-CD4 stained on T helper cells.
Panel D : anti-CD19 stained on B cells.

Sections were treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins before antibody incubation. The section was incubated in three rounds of staining : in the order of ab245118 for 30 mins, then ab238798 and ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

DAPI was used as a nuclear counterstain.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

This data was developed using ab237772, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human tonsil tissue labeling CD8 alpha with ab245118 at 1/500 dilution, CD4 with ab238798 at 1/500, and CD19 with ab237772 at 1/5000 dilution.

Panel A : merged staining of anti-CD8 alpha (magenta; Opal™690), anti-CD4 (green; Opal™520) and anti-CD19 (red; Opal™570) on human tonsil.
Panel B : anti-CD8 alpha stained on cytotoxic T cells.
Panel C : anti-CD4 stained on T helper cells.
Panel D : anti-CD19 stained on B cells.

Sections were treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins before antibody incubation. The section was incubated in three rounds of staining : in the order of ab245118 for 30 mins, then ab238798 and ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

DAPI was used as a nuclear counterstain.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

Panel A : merged staining of anti-CD68 (magenta; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD19 (red; Opal™570) on Formalin/PFA-fixed paraffin-embedded sections of human spleen. Secondary antibody was Opal Polymer HRP Ms + Rb, and couterstaining was with DAPI. Panel B : anti-CD3 epsilon stained on T cells with ab16669 at 1/500 dilution Panel C : anti-CD19 stained on B cells with ab237772 at 1/5000 dilution Panel D : anti-CD68 stained on macrophages with ab213363 1/500 dilution The section was incubated in three rounds of staining : in the order of ab213363 and ab16669 for 30 mins, then ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human spleen tissue staining TCR delta with ab313573 at a 1/500 dilution, ab16669 anti-CD3 used at 1/100 dilution and ab227688 anti-CD19 used at a 1/100 dilution.

Panel A : merged staining of anti-TCR delta (green; Opal^™520), anti-CD3 (magenta; Opal^™570) and anti-CD19 (yellow; Opal^™690) on human colon.
Panel B : anti-TCR delta staining immune cells in human colon.
Panel C : anti-CD3 staining T lymphocytes in human colon.
Panel D : anti-CD19 staining B lymphocytes in human colon.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab313573, ab16669 and ab227688 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins..

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

Formalin-fixed, paraffin-embedded human thymus tissue stained for CD19 using ab227688 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

Formalin-fixed, paraffin-embedded human spleen tissue stained for CD19 using ab227688 at 1/100 dilution in immunohistochemical analysis.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

Flow Cytometry analysis of Ramos (human Burkitt's lymphoma cell line) cells, labeling CD19 with ab227688 at 1/400 dilution (green) compared to a Rabbit IgG negative control (blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

Formalin-fixed, paraffin-embedded human B-Cell lymphoma tissue stained for CD19 using ab227688 at 1/100 dilution in immunohistochemical analysis.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

Flow cytometry analysis of Raji (human Burkitt's lymphoma) labeling CD19 with purified ab227688 at 1/20 dilution (7.5 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for CD19 using ab227688 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

The BOND™ Polymer Refine Detection System on the BOND RX Automated Stainer (DS9800, Leica Biosystems, Wetzlar, Germany) was used for immunohistochemical labelling of formalin-fixed, paraffin-embedded (FFPE) tissues. The protocol includes a peroxide block, post-primary reagent, polymer detection, DAB chromogen, and haematoxylin counterstain, all automated to minimize variability. Antigen retrieval was performed by heat-induced epitope retrieval (HIER) for 20 minutes in Tris-EDTA buffer (BOND Epitope Retrieval Solution 2, AR9640, Leica Biosystems, Wetzlar, Germany). For mouse and rabbit antibodies, the sequence comprised peroxide block (10 min), primary antibody incubation (30 min), post-primary (10 min), polymer (10 min), DAB (10 min), and haematoxylin (8 min). For rat antibodies, an anti-rat step (1 : 300 dilution, Vector Laboratories, California, USA; 20 min) was included prior to polymer application (15 min).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

Formalin-fixed, paraffin-embedded human colon tissue stained for CD19 using ab227688 at 1/100 dilution in immunohistochemical analysis.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

This data was developed using ab237772, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver cancer labelling CD208 with ab281573 at 1/1200 (B), CD3E with ab237707 at 1/1200 dilution (C) and CD19 with ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human liver cancer.

Panel B : anti-CD208 staining dendritic cells in human liver cancer.

Panel C : anti-CD3E staining T lymphocytes in human liver cancer.

Panel D : anti-CD19 staining B lymphocytes of human liver cancer.

The section was incubated in three rounds of staining : in the order ab281573, ab237707, and ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

This data was developed using ab237772, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil labelling CD208 with ab281573 at 1/1200 (B), CD3E with ab237707 at 1/1200 dilution (C) and CD19 with ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human tonsil.
Panel B : anti-CD208 staining dendritic cells in human tonsil.
Panel C : anti-CD3E staining T lymphocytes in human tonsil.
Panel D : anti-CD19 staining B lymphocytes of human tonsil.

The section was incubated in three rounds of staining : in the order ab281573, ab237707, and ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

This data was developed using ab237772, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon labelling CD208 with ab281573 at 1/1200 (B), CD3E with ab237707 at 1/1200 dilution (C) and CD19 with ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human colon.

Panel B : anti-CD208 staining dendritic cells in human colon.

Panel C : anti-CD3E staining T lymphocytes in human colon.

Panel D : anti-CD19 staining B lymphocytes of human colon.

The section was incubated in three rounds of staining : in the order ab281573, ab237707, and ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

This data was developed using ab237772, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon cancer labelling CD208 with ab281573 at 1/1200 (B), CD3E with ab237707 at 1/1200 dilution (C) and CD19 with ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human colon cancer.

Panel B : anti-CD208 staining dendritic cells in human colon cancer.

Panel C : anti-CD3E staining T lymphocytes in human colon cancer.

Panel D : anti-CD19 staining B lymphocytes of human colon cancer.

The section was incubated in three rounds of staining : in the order ab281573, ab237707, and ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

Panel A : merged staining of anti-CD68 (gray; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD19 (red; Opal™570) on Formalin/PFA-fixed paraffin-embedded sections of human tonsil. Secondary antibody was Opal Polymer HRP Ms + Rb, and counterstaining was with DAPI. Panel B : anti-CD3 epsilon stained on T cells with ab16669 at 1/500 dilution Panel C : anti-CD19 stained on B cells with ab237772 at 1/5000 dilution Panel D : anti-CD68 stained on macrophages with ab213363 1/500 dilution The section was incubated in three rounds of staining : in the order of ab213363 and ab16669 for 30 mins, then ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

Panel A : merged staining of anti-CD31 (gray; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD19 (red; Opal™570) on Formalin/PFA-fixed paraffin-embedded sections of human tonsil. Secondary antibody was Opal Polymer HRP Ms + Rb, nuclear counterstain was DAPI. Panel B : anti-CD3 epsilon stained on T cells with ab16669 at 1/500 dilution Panel C : anti-CD19 stained on B cells with ab237772 at 1/5000 dilution Panel D : anti-CD31 stained on endothelial cells and immune cell subsets with ab207090 at 1/500 dilution The section was incubated in three rounds of staining : in the order of ab207090 and ab16669 for 30 mins, then ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human hodgkin's lymphoma tissue staining CD72 with ab324351 at a 1 : 500 (1.018 ug/ml) dilution, ab227688 anti-CD19 used at 1 : 100 (0.15 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.

Panel A : merged staining of anti-CD72 (green; Opal™520), anti-CD19 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on human Hodgkin's lymphoma.
Panel B : anti-CD72 staining immune cells in human Hodgkin's lymphoma.
Panel C : ant-CD19 staining B lymphocytes in human Hodgkin's lymphoma.
Panel D : ant-CD3 staining T lymphocytes in human Hodgkin's lymphoma.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324351, ab227688 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human spleen tissue staining TCR delta with ab313573 at a 1/500 dilution, ab16669 anti-CD3 used at 1/100 dilution and ab227688 anti-CD19 used at a 1/100 dilution.

Panel A : merged staining of anti-TCR delta (green; Opal^™520), anti-CD3 (magenta; Opal^™570) and anti-CD19 (yellow; Opal^™690) on human spleen.
Panel B : anti-TCR delta staining immune cells in human spleen.
Panel C : anti-CD3 staining T lymphocytes in human spleen.
Panel D : anti-CD19 staining B lymphocytes in human spleen.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab313573, ab16669 and ab227688 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

This data was developed using ab237772, the same antibody clone in a different buffer formulation.

Panel A : merged staining of anti-CD19 (green; Opal™520), anti-CD19 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on human Hodgkin's lymphoma. Panel B : anti-CD19 staining the B lymphocytes in human Hodgkin's lymphoma. Panel C : anti-CD19 staining the B lymphocytes in human Hodgkin's lymphoma. Panel D : anti-CD3 staining the T lymphocytes in human Hodgkin's lymphoma. Nuclear DNA was labeled with DAPI (shown in blue). The section was incubated in three rounds of staining : in the order of ab320735 at 1/2000 dilution, ab237772 at 1/5000 dilution and ab16669 at 1/150 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The secondary used was Opal Polymer HRP Ms + Rb. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle labeling CD19 with ab227688 at 1/100 (0.15 ug/ml) dilution, followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Negative control : no staining in human skeletal muscle.
The section was incubated with ab227688 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

This data was developed using ab237772, the same antibody clone in a different buffer formulation.

Panel A : merged staining of anti-CD19 (green; Opal™520), anti-CD19 (green; Opal™570) and anti-CD3 (yellow; Opal™690) on human cerebrum. Panel B : anti-CD19 showed no staining in human cerebrum. Panel C : anti-CD19 showed no staining in human cerebrum. Panel D : anti-CD3 showed no staining in human cerebrum. Nuclear DNA was labeled with DAPI (shown in blue). The section was incubated in three rounds of staining : in the order of ab320735 at 1/2000 dilution, ab237772 at 1/5000 dilution and ab16669 at 1/150 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The secondary used was Opal Polymer HRP Ms + Rb. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

This data was developed using ab237772, the same antibody clone in a different buffer formulation.

Panel A : merged staining of anti-CD19 (green; Opal™520), anti-CD19 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on human tonsil. Panel B : anti-CD19 staining the B lymphocytes in human tonsil. Panel C : anti-CD19 staining the B lymphocytes in human tonsil. Panel D : anti-CD3 staining the T lymphocytes in human tonsil. Nuclear DNA was labeled with DAPI (shown in blue). The section was incubated in three rounds of staining : in the order of ab320735 at 1/2000 dilution, ab237772 at 1/5000 dilution and ab16669 at 1/150 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The secondary used was Opal Polymer HRP Ms + Rb. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• WB

Supplier Data

Western blot - Anti-CD19 antibody [SP291] - C-terminal (AB227688)

All lanes:

Western blot - Anti-CD19 antibody [SP291] - C-terminal (ab227688) at 1/400 dilution

All lanes:

Ramos (human Burkitt's lymphoma cell line) cell lysate

Predicted band size: 61 kDa

false