JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB324082

Anti-CD19 抗体 [EPR29472-65] - BSA and Azide free (Detector)

Anti-CD19 antibody [EPR29472-65] - BSA and Azide free (Detector)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal CD19 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

別名を表示する

CD19, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12

3 Images
Sandwich ELISA - Anti-CD19 antibody [EPR29472-65] - BSA and Azide free (Detector) (AB324082)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-CD19 antibody [EPR29472-65] - BSA and Azide free (Detector) (AB324082)

Sandwich ELISA using Human CD19 immunogen protein standard curve with the capture antibody ab324080 at 2 ug/mL and detector antibody ab324082 at 0.5 ug/mL. Background-subtracted absorbance data values (mean +/- standard deviation are shown.)

Sandwich ELISA - Anti-CD19 antibody [EPR29472-65] - BSA and Azide free (Detector) (AB324082)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-CD19 antibody [EPR29472-65] - BSA and Azide free (Detector) (AB324082)

Interpolated concentration of native CD19 was measured at different sample concentrations. Undiluted sample is as follows : Raji cell extract (0.25 mg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-CD19 antibody [EPR29472-65] - BSA and Azide free (Detector) (AB324082)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-CD19 antibody [EPR29472-65] - BSA and Azide free (Detector) (AB324082)

Interpolated concentration of native CD19 was measured at different sample concentrations. Undiluted sample is as follows : Daudi cell extract (0.25 mg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR29472-65

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

製品の詳細

ab324082 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human CD19. The recommended pair for sandwich ELISA is:

Capture: ab324080, Human CD19 Capture Antibody (unconjugated)

Detector: ab324082, Human CD19 Detector Antibody (unconjugated)

The reference range value is 19.5-5000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
style
left-column
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes (PubMed : 29523808). Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed : 1373518, PubMed : 16672701, PubMed : 2463100). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed : 12387743, PubMed : 16672701, PubMed : 9317126, PubMed : 9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed : 9317126). Required for normal differentiation of B-1 cells (By similarity). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed : 1373518, PubMed : 2463100). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed : 12387743, PubMed : 16672701, PubMed : 9317126).
See full target information CD19
style
left-column
style
left-column
style
right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com