Anti-CD18 抗体 [MEM-48] - Low endotoxin, Azide free (ab46700)
![Flow Cytometry - Anti-CD18 antibody [MEM-48] - Low endotoxin, Azide free (ab46700)](https://www.abcam.co.jp/ps/products/46/ab46700/Images/ab46700-58934-anti-cd18-antibody-mem-48-flow-cytometry.jpg "Flow Cytometry - Anti-CD18 antibody [MEM-48] - Low endotoxin, Azide free (ab46700)")
Key features and details
- Mouse monoclonal [MEM-48] to CD18 - Low endotoxin, Azide free
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-CD18 antibody [MEM-48] - Low endotoxin, Azide free
CD18 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MEM-48] to CD18 - Low endotoxin, Azide free -
由来種
Mouse -
特異性
The antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin ß2 subunit; ß2 integrin). -
アプリケーション
適用あり: IHC-P, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to CD18. Leucocytes of a patient suffering from a LGL-type leukaemia.
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ポジティブ･コントロール
- WB: Kg-1a human leukemia cell lysate
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特記事項
Preserved by filter sterilizationThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Multi-step Chromatography -
特記事項（精製）
Purified from TCS. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
MEM-48 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46700の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
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IHC-P
Use at an assay dependent concentration. |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. -
関連疾患
Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions. -
配列類似性
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
翻訳後修飾
Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3689 Human
- Omim: 600065 Human
- SwissProt: P05107 Human
- Unigene: 375957 Human
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別名
- 95 subunit beta antibody
- Beta 2 (antigen CD18 (p95) antibody
- CD 18 antibody
see all
画像
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Flow Cytometry - Anti-CD18 antibody [MEM-48] - Low endotoxin, Azide free (ab46700)
Overlay histogram showing peripheral blood lymphocytes stained with ab46700 (red line). The cells were incubated with the antibody (ab46700, 1µg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/200 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab46700 は 1 報の論文で使用されています。
- Kuttruff S et al. NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood 113:358-69 (2009). PubMed: 18922855
