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Anti-CD18 抗体 [MEM-148] (ab8220)

Reviews (1)Q&A (3)References (14)
Flow Cytometry - Anti-CD18 antibody [MEM-148] (ab8220)
  • Flow Cytometry - Anti-CD18 antibody [MEM-148] (ab8220)

Key features and details

  • Mouse monoclonal [MEM-148] to CD18
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

APC PE

製品の概要

  • 製品名

    Anti-CD18 antibody [MEM-148]
    CD18 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [MEM-148] to CD18

  • 由来種

    Mouse

  • 特異性

    Recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1.

  • アプリケーション

    適用あり: Flow Cytmore details

  • 種交差性

    交差種: Human
    非交差種: Pig, Monkey

  • 免疫原

    Tissue, cells or virus corresponding to Human CD18. Peripheral blood mononuclear cells

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab8220の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use 0.1µg for 106 cells.

The antibody is excellent marker of activated myeloid cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
特記事項
Flow Cyt
Use 0.1µg for 106 cells.

The antibody is excellent marker of activated myeloid cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ターゲット情報

  • 機能

    Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.

  • 関連疾患

    Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.

  • 配列類似性

    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.

  • 翻訳後修飾

    Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 95 subunit beta antibody
      • Beta 2 (antigen CD18 (p95) antibody
      • CD 18 antibody
      • CD18 antibody
      • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody
      • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody
      • Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody
      • Complement component 3 receptor 3 and 4 subunit antibody
      • Complement receptor C3 beta subunit antibody
      • Complement receptor C3 subunit beta antibody
      • Integrin beta 2 antibody
      • Integrin beta chain beta 2 antibody
      • Integrin beta-2 (ITGB2) antibody
      • Integrin beta-2 antibody
      • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody
      • ITB2_HUMAN antibody
      • ITGB2 antibody
      • LAD antibody
      • LCAMB antibody
      • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody
      • Leukocyte cell adhesion molecule CD18 antibody
      • LFA 1 antibody
      • LFA1 antibody
      • Lymphocyte function associated antigen 1 (LFA1) antibody
      • Lymphocyte function associated antigen 1 antibody
      • MAC 1 antibody
      • MAC1 antibody
      • Macrophage antigen 1 (mac1) beta antibody
      • MF17 antibody
      • MFI7 antibody
      • OTTHUMP00000115278 antibody
      • OTTHUMP00000115279 antibody
      • OTTHUMP00000115280 antibody
      • OTTHUMP00000115281 antibody
      • OTTHUMP00000115282 antibody
      see all

    画像

    • Flow Cytometry - Anti-CD18 antibody [MEM-148] (ab8220)
      Flow Cytometry - Anti-CD18 antibody [MEM-148] (ab8220)

      Flow cytometry analysis of human peripheral whole blood cells using ab8220 at 1.67 µg/ml showing separation of human monocytes (red-filled) from CD18 negative blood debris (black-dashed).

    • Flow Cytometry - Anti-CD18 antibody [MEM-148] (ab8220)
      Flow Cytometry - Anti-CD18 antibody [MEM-148] (ab8220)

      Human peripheral blood lymphocytes stained with ab8220 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab8220, 0.1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

    参考文献 (14)

    ab8220 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab8220 は 14 報の論文で使用されています。

    • Cha M  et al. Quantitative imaging of vesicle-protein interactions reveals close cooperation among proteins. J Extracell Vesicles 12:e12322 (2023). PubMed: 37186457
    • Baganha F  et al. Phosphorylcholine Monoclonal Antibody Therapy Decreases Intraplaque Angiogenesis and Intraplaque Hemorrhage in Murine Vein Grafts. Int J Mol Sci 23:N/A (2022). PubMed: 36362449
    • Baganha F  et al. Atorvastatin pleiotropically decreases intraplaque angiogenesis and intraplaque haemorrhage by inhibiting ANGPT2 release and VE-Cadherin internalization. Angiogenesis 24:567-581 (2021). PubMed: 33550461
    • Li D  et al. ß-1,3-Glucan/CR3/SYK pathway-dependent LC3B-II accumulation enhanced the fungicidal activity in human neutrophils. J Microbiol N/A:N/A (2019). PubMed: 30721460
    • Trentini A  et al. Hydroxyethyl Starch 130/0.4 Binds to Neutrophils Impairing Their Chemotaxis through a Mac-1 Dependent Interaction. Int J Mol Sci 20:N/A (2019). PubMed: 30769810
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-CD18 antibody [MEM-148]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hep-3B,HLF)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    20 µg
    Treatment
    25 µM BSO for 24hrs
    Specification
    Hep-3B,HLF
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Chengpeng Yu

    Verified customer

    投稿 Nov 26 2020

    Question

    ab8220 detects multiple bands in WB

    Read More
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ***.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Question

    Dear technical service, I would appreciate information concerning the number of cells (neutrophils) and levels of antibodies that you would recommend using for the following antibodies: ab8220 primary antibody ab6785 secondary ab7002-500 secondary We will further test saturation for our cells in our laboratory but would appreciate your initial advice.

    Read More
    Answer

    There is a citation from the published methodology (Drbal et al. Blood 98(5), pp1561-1566, 2001): mAb used in paper: MEM-148 (ab8220) MEM-48 (standard anti CD18, #11-216) and other mAbs supplied by Abcam Cell were stained with mAbs (20 ug/ml) for 30 min on ice and washed in HBSS (Hanks balanced salt solution) containing 0.2% gelatin and 0.1% NaN3 without Ca++ and Mg++ followed by fluorescein labeled antimouse Ig. Propidium iodide (0.1 ug/ml) and LDS-751 (0.2 ug/ml) were added prior to measurement on FACS in a standard 3-color setup. At least 10000 viable nucleated cells (PI- , LDS-751+) were collected for each sample. Leucocyte populations were resolved on the basis of their scatter properties.

    Read More

    Question

    Technical Services, Does CD18-ab8220 also recognize the high affinity state of Mac-1(CD11b/CD18)? Your product data sheet states that it recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high affinity state of LFA-1 (CD11a/CD18).

    Read More
    Answer

    It is not clear whether ab8220 recognizes the high-affinity (activated) conformation also on Mac-1, we have only indirect indications that it is the case but no definitive unambiguous data

    Read More

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