Anti-CD18 抗体 [C71/16] (ab19580)
![Flow Cytometry - Anti-CD18 antibody [C71/16] (ab19580)](https://www.abcam.co.jp/ps/products/19/ab19580/Images/ab19580-1-anti-cd18-antibody-c7116-flow-cytometry-mouse-peripheral-blood-mononuclear-cell.jpg "Flow Cytometry - Anti-CD18 antibody [C71/16] (ab19580)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [C71/16] to CD18
- Suitable for: Flow Cyt
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-CD18 antibody [C71/16]
CD18 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [C71/16] to CD18 -
由来種
Rat -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Flow Cyt: Mouse peripheral blood mononuclear cell (PBMC).
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Ion Exchange Chromatography -
ポリ/モノ
モノクローナル -
クローン名
C71/16 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab19580の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use a concentration of 1.003 µg/ml.
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| 特記事項 |
|---|
|
Flow Cyt
Use a concentration of 1.003 µg/ml. |
ターゲット情報
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機能
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. -
関連疾患
Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions. -
配列類似性
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
翻訳後修飾
Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 16414 Mouse
- SwissProt: P11835 Mouse
- Unigene: 1137 Mouse
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別名
- 95 subunit beta antibody
- Beta 2 (antigen CD18 (p95) antibody
- CD 18 antibody
see all
画像
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Flow Cytometry - Anti-CD18 antibody [C71/16] (ab19580)
Flow cytometric analysis of mouse peripheral blood mononuclear cell (PBMC) cells labelling CD18 with ab19580 at 1/1000 dilution (1.003µg/ml) (Right panel) compared with a rat monoclonal IgG isotype control (Left panel). Goat F(ab)2 Anti-rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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![Flow Cytometry - Anti-CD18 antibody [C71/16] (ab19580)](https://www.abcam.co.jp/ps/products/19/ab19580/Images/ab19580-2-anti-cd18-antibody-c7116-flow-cytometry-mouse-peripheral-blood-mononuclear-cell1.jpg)
Flow Cytometry - Anti-CD18 antibody [C71/16] (ab19580)Flow cytometric analysis of mouse peripheral blood mononuclear cell (PBMC) cells labelling CD18 with ab19580 at 1/1000 dilution (1.003µg/ml) (Red) compared with a rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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![Anti-CD18 antibody [C71/16] (ab19580)](https://www.abcam.co.jp/ps/products/19/ab19580/Images/ab19580-3-benefits-of-recombinant-antibodies.png)
Anti-CD18 antibody [C71/16] (ab19580)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab19580 は論文での使用が確認できていません。