Anti-CD163 抗体 [RM3/1] (ab17051)

Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information or see the link below:

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Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

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Thank you for your enquiry and your interest in our products.

Generally, each antibody and batch should be titrated to determine the optimal working dilutions/concentrations.

ab17051: this antibody has only been tested in ICC/IF and IHC/Fr but it is unsuitable for formalin-fixed paraffin-embedded sections. The unit size is 50 µg at 0.1mg/ml and it is suggested to apply for IHC-Fr at aconcentration of 4 µg/ml (please read application notes).

ab21562:this antibody has only been tested in IHC/Fr but it is unsuitable for formalin-fixed paraffin-embedded sections. The unit size is 100 µg at 0.5mg/ml but the working dilution needs to be optimized by the end user.

If you need any further assistance in the future, please do not hesitate to contact me.

Thank you for contacting us. The antibody ab17051 has been tested with rat samples and turned out to not be working for this species (indicated under "Does not react with"). The other antibodies for CD163 have not been tested with rat so far. I do not have the rat sequence to check sequence homology. If you could sent me the sequence, I'd be happy to check the homology between human and rat for you. And if the homology is 85% or higher (giving a good chance of cross reactivity), you can try one of the antibodies with rat samples and use our testing discount program. Here is some information about how the testing discount program works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for getting back to me with further details. I appreciate the steps that you have taken to optimise this antibody and the control stainings that you detail. I am certainly prepared to offer you a credit note against this purchase provided that the antibody was purchased within the past 90 days. If this is the case please email me details of the order including the order number and date of purchase. I look forward to hearing from you.

Thank you for your enquiry. I am sorry to the delay to your enquiry and sorry to hear that you have been having difficulties with this antibody. I have been in touch with the originator and they have offered the following suggestions. CD163 is a membrane glycoprotein, which is expressed exclusively on the cell surface of human monocytes and macrophages. Therefore no antigen retrieval step should be necessary. CD163 is sensitive to ionic detergents and chloroform/methanol treatment, this is not just for Western Blot but also for IHC-P protocols. Can I ask whether you tested another antigen on the cells as positive control, to determine whether your procedure was ok? At this stage I would like to suggest that you perform the test one more time without antigen retrieval using an overnight incubation. Should your results not improve please get back in touch with me.