AB87099

Anti-CD163 抗体

Name: Anti-CD163 抗体 (ab87099) | Abcam
Brand: Abcam Limited
SKU: AB87099
Price: 61000 JPY
Availability: InStock
Rating: 5 (6 reviews)

Anti-CD163 antibody

(6 Reviews)

(106 Publications)

Rabbit Polyclonal CD163 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 106 publications.

別名を表示する

CD163, M130, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-CD163 antibody (AB87099)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD163 antibody (AB87099)

ab87099 stained in THP1 PMA treated cells. Cells were fixed with 4% paraformaldehyde (10 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% Triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab87099 at 5μg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 μM for 1hour at room temperature.

Unknown

Western blot - Anti-CD163 antibody (AB87099)

All lanes:

Western blot - Anti-CD163 antibody (ab87099) at 1 µg/mL

Lane 1:

Human spleen tissue lysate - total protein (<a href='/products/unavailable/human-spleen-tissue-lysate-total-protein-ab29699'>ab29699</a>) at 10 µg

Lane 2:

Human thymus tissue lysate - total protein (ab30146) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 125 kDa

Observed band size: 12 kDa,150 kDa

true

Exposure time: 20min

Unknown

Western blot - Anti-CD163 antibody (AB87099)

All lanes:

Western blot - Anti-CD163 antibody (ab87099) at 2 µg/mL

Lane 1:

Human thymus tissue lysate - total protein (ab30146) at 20 µg

Lane 2:

Human spleen tissue lysate - total protein (<a href='/products/unavailable/human-spleen-tissue-lysate-total-protein-ab29699'>ab29699</a>) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 125 kDa

Observed band size: 150 kDa,35 kDa,45 kDa,55 kDa

true

Exposure time: 20min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1-5 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Macaque monkey": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD163 known also as hemoglobin scavenger receptor is a membrane glycoprotein with a molecular weight of approximately 130 kDa. It is predominantly expressed by monocytes and macrophages which are types of immune cells. Located on the cell surface CD163 functions as a receptor that aids in the clearance of hemoglobin-haptoglobin complexes playing a critical role in maintaining iron homeostasis and protecting tissues from oxidative damage. CD163 is not limited to human cells only; researchers often study its function using animal models such as chimeric rodents and chimeric rats.

Biological function summary

CD163 participates in anti-inflammatory processes and is linked to immune modulation. It plays a pivotal role in the resolution of inflammation and promotes the removal of damaged cells and tissues. Through its involvement in the endocytic pathway CD163 interacts with various ligands facilitating interactions with other proteins. In the context of research CD163 functionality can be analyzed using techniques like CD163 IHC CD163 ELISA and CD163 stain in both human and mouse models.

Pathways

CD163 is integral to the inflammation and immune response pathways. It participates in the heme-oxygenase pathway which is essential in the breakdown of heme into biliverdin free iron and carbon monoxide. This pathway involvement is tightly linked with heme oxygenase-1 (HO-1) a cytoprotective enzyme that helps to mitigate inflammatory responses. The interaction with haptoglobin and related proteins provides further insight into its regulatory roles within the immune system.

CD163 is associated with conditions like atherosclerosis and acute inflammation disorders. Its elevated levels indicate macrophage activation observed in chronic inflammations such as cardiovascular diseases. In atherosclerosis CD163 expression correlates with the uptake of oxidized LDL connecting it to progression of the disease. Additionally soluble CD163 levels serve as a biomarker in conditions like chronic liver disease where it is linked to macrophage activation and fibrosis. Within these disorders proteins like LDL and inflammatory cytokines are closely associated with CD163 function and regulation.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

See full target information CD163

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文献 (106)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40643489

2025

Effects of Obeticholic Acid Treatment on Primary Human Hepatocytes in a Novel Tri-Culture Model System.

Applications

Unspecified application

Species

Unspecified reactive species

Justin J Odanga,Sharon M Anderson,Edward L LeCluyse,Sharon C Presnell,Jingsong Chen,Jessica R Weaver

Frontiers in oncology 15:1554845 PubMed40548122

2025

Interrogation of macrophage-related prognostic signatures reveals a potential immune-mediated therapy strategy by histone deacetylase inhibition in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Xisen Wang,Xuya Wang,Lei Chen,Haozhe Ding,Jikang Fan,Jianshen Liang,Yu Zhang,Yiming Li,Yiming Zhang,Shengping Yu,Chen Zhang,Yaohua Li,Tao Li,Xuejun Yang

Cellular and molecular neurobiology 45:47 PubMed40394428

2025

Atorvastatin Protects Against the Macrophage/Microglia-Related Neuroinflammation via Inhibiting Lipocalin-2 in Mouse Experimental Intracerebral Hemorrhage Model.

Applications

Unspecified application

Species

Unspecified reactive species

Guangming Wang,Hongkang Hu,Junbin Liu,Xiaowei Fei,Yanan Dou,Li Wang,Lin Ying,Guohan Hu,Danfeng Zhang,Lei Jiang,Jialiang Wei

STAR protocols 6:103760 PubMed40238632

2025

Protocol for characterizing craniopharyngioma subtypes and their microenvironments using single-cell RNA sequencing and immunohistochemistry.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Kono,Tatsuma Matsuda,Masanori Fujimoto,Yuki Taki,Ikki Sakuma,Naoko Hashimoto,Yasuhiro Nakamura,Kentaro Horiguchi,Yoshinori Higuchi,Atsushi Onodera,Takashi Miki,Tomoaki Tanaka

Pharmaceuticals (Basel, Switzerland) 17: PubMed39770467

2025

Impact of Post-Thaw Enrichment of Primary Human Hepatocytes on Steatosis, Inflammation, and Fibrosis in the TruVivo System.

Applications

Unspecified application

Species

Unspecified reactive species

Justin J Odanga,Sharon M Anderson,Sharon C Presnell,Edward L LeCluyse,Jingsong Chen,Jessica R Weaver

Cancer immunology, immunotherapy : CII 74:55 PubMed39751840

2025

CCR5 and IL-12 co-expression in CAR T cells improves antitumor efficacy by reprogramming tumor microenvironment in solid tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Yonggui Tian,Liubo Zhang,Yu Ping,Zhen Zhang,Chang Yao,Chunyi Shen,Feng Li,Chunli Wen,Yi Zhang

Cancer immunology, immunotherapy : CII 74:37 PubMed39738657

2025

Targeting OAS3 for reversing M2d infiltration and restoring anti-tumor immunity in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shaopeng Zhang,Ximo Xu,Kundong Zhang,Changzheng Lei,Yitian Xu,Pengshan Zhang,Yuan Zhang,Haitao Gu,Chen Huang,Zhengjun Qiu

iScience 27:111068 PubMed39483146

2024

Deciphering craniopharyngioma subtypes: Single-cell analysis of tumor microenvironment and immune networks.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuma Matsuda,Takashi Kono,Yuki Taki,Ikki Sakuma,Masanori Fujimoto,Naoko Hashimoto,Eiryo Kawakami,Noriaki Fukuhara,Hiroshi Nishioka,Naoko Inoshita,Shozo Yamada,Yasuhiro Nakamura,Kentaro Horiguchi,Takashi Miki,Yoshinori Higuchi,Tomoaki Tanaka

STAR protocols 5:103359 PubMed39368096

2024

Protocol for isolating CD163 Kupffer cells from human liver resections.

Applications

Unspecified application

Species

Unspecified reactive species

Armando Andres Roca Suarez,Marie-Laure Plissonnier,Maud Michelet,Anaëlle Dubois,Sarah Heintz,Maria Saez-Palma,Marion Delphin,Isabelle Bordes,Jennifer Molle,Audrey Diederichs,Mélanie Rodà,Emmanuel Combe,Léa Mougené,Guillaume Giraud,Xavier Grand,Michel Rivoire,Massimo Levrero,Barbara Testoni,Fabien Zoulim

Journal of personalized medicine 14: PubMed39338219

2024

Characterization of Tissue Immunity Defense Factors of the Lip in Primary Dentition Children with Bilateral Cleft Lip Palate.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Ozola,Mara Pilmane

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CD163 抗体

Anti-CD163 antibody

Immunocytochemistry/ Immunofluorescence - Anti-CD163 antibody (AB87099)

Western blot - Anti-CD163 antibody (AB87099)

Western blot - Anti-CD163 antibody (AB87099)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (106)

Abcam product promise