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Anti-CD16 抗体 [MEM-154] - BSA and Azide free (ab46679)

Submit a review Q&A (2)References (4)
Flow Cytometry - Anti-CD16 antibody [MEM-154] - Azide free (ab46679)
  • Flow Cytometry - Anti-CD16 antibody [MEM-154] - BSA and Azide free (ab46679)
  • Flow Cytometry - Anti-CD16 antibody [MEM-154] - BSA and Azide free (ab46679)

Key features and details

  • Mouse monoclonal [MEM-154] to CD16 - BSA and Azide free
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-CD16 antibody [MEM-154] - BSA and Azide free
    CD16 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [MEM-154] to CD16 - BSA and Azide free

  • 由来種

    Mouse

  • 特異性

    The antibody MEM-154 reacts with the epitope on CD16 antigen residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcγRIII antigen). MEM-154 recognizes a polymorphism of CD16a (NK cells form), it reacts well with granulocyte form (CD16b) of all subjects. The antibody blocks binding of human IgG to FcγRIII.

  • アプリケーション

    適用あり: Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Tissue, cells or virus corresponding to Human CD16. Human granulocytes

  • ポジティブ･コントロール

    • Peripheral blood lymphocytes

  • 特記事項

    Preserved by filter sterilization.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab46679の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
特記事項
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

  • 組織特異性

    Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.

  • 配列類似性

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

  • 翻訳後修飾

    Glycosylated. Contains high mannose- and complex-type oligosaccharides.
    The soluble form is produced by a proteolytic cleavage.

  • 細胞内局在

    Cell membrane. Secreted. Exists also as a soluble receptor.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD 16 antibody
      • CD 16a antibody
      • CD16 antibody
      • CD16a antibody
      • CD16a antigen antibody
      • CD16B antibody
      • CD16b antigen antibody
      • Fc fragment of IgG antibody
      • Fc fragment of IgG low affinity IIIa receptor (CD16) antibody
      • Fc fragment of IgG low affinity IIIa receptor antibody
      • Fc fragment of IgG receptor IIIa antibody
      • Fc fragment of IgG, low affinity III, receptor (CD16) antibody
      • Fc fragment of IgG, low affinity III, receptor for (CD16) antibody
      • Fc fragment of IgG, low affinity IIIa, receptor (CD16) antibody
      • Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antibody
      • Fc fragment of IgG, low affinity IIIa, receptor for antibody
      • Fc fragment of IgG, low affinity IIIb, receptor (CD16b) antibody
      • Fc fragment of IgG, low affinity IIIb, receptor for (CD16) antibody
      • Fc gamma R3 antibody
      • Fc gamma receptor III 2 (CD 16) antibody
      • Fc gamma receptor III A antibody
      • Fc gamma receptor IIIA antibody
      • Fc gamma receptor IIIb (CD 16) antibody
      • Fc gamma RIII alpha antibody
      • Fc gamma RIII antibody
      • Fc gamma RIII beta antibody
      • Fc gamma RIIIa antibody
      • Fc gamma RIIIb antibody
      • Fc of IgG antibody
      • Fc-gamma receptor III2 (CD 16) antibody
      • Fc-gamma receptor III2 (CD16) antibody
      • Fc-gamma receptor IIIb (CD16) antibody
      • Fc-gamma RIII antibody
      • Fc-gamma RIII-alpha antibody
      • Fc-gamma RIIIa antibody
      • FCG 3 antibody
      • FCG3 antibody
      • FCG3A_HUMAN antibody
      • FCgammaRIIIA antibody
      • FCGR 3 antibody
      • FCGR 3A antibody
      • FCGR3 antibody
      • FCGR3A antibody
      • FCGR3A protein antibody
      • FCGRIII antibody
      • FCGRIII-2 antibody
      • FcR 10 antibody
      • FcR-10 antibody
      • FcR10 antibody
      • FcRIII antibody
      • FcRIIIa antibody
      • IGFR 3 antibody
      • IGFR3 antibody
      • IgG Fc receptor III 1 antibody
      • IgG Fc receptor III 2 antibody
      • IgG Fc receptor III-2 antibody
      • IMD20 antibody
      • immunoglobulin G Fc receptor III antibody
      • immunoglobulin G Fc receptor III-2 antibody
      • Low affinity IIIa receptor antibody
      • Low affinity immunoglobulin gamma Fc region receptor III A antibody
      • Low affinity immunoglobulin gamma Fc region receptor III-A antibody
      • Low affinity immunoglobulin gamma Fc region receptor IIIB antibody
      • neutrophil-specific antigen NA antibody
      see all

    画像

    • Flow Cytometry - Anti-CD16 antibody [MEM-154] - Azide free (ab46679)
      Flow Cytometry - Anti-CD16 antibody [MEM-154] - Azide free (ab46679)

      Human peripheral blood granulocytes stained with ab46679 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab46679, 1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood granulocytes.

    • Flow Cytometry - Anti-CD16 antibody [MEM-154] - BSA and Azide free (ab46679)
      Flow Cytometry - Anti-CD16 antibody [MEM-154] - BSA and Azide free (ab46679)
      Flow cytometry surface staining pattern of human peripheral blood stained using ab46679 at 2 μg/ml) GAM APC.
       

       

    • Flow Cytometry - Anti-CD16 antibody [MEM-154] - BSA and Azide free (ab46679)
      Flow Cytometry - Anti-CD16 antibody [MEM-154] - BSA and Azide free (ab46679)

      Separation of human neutrophil granulocytes (red-filled) from CD16 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab46679 at 2 μg/ml.

    参考文献 (4)

    ab46679 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab46679 は 4 報の論文で使用されています。

    • Zhang PP  et al. COVID-19-associated monocytic encephalitis (CAME): histological and proteomic evidence from autopsy. Signal Transduct Target Ther 8:24 (2023). PubMed: 36609561
    • Wu S  et al. The long noncoding RNA LINC01140/miR-140-5p/FGF9 axis modulates bladder cancer cell aggressiveness and macrophage M2 polarization. Aging (Albany NY) 12:25845-25864 (2020). PubMed: 33234721
    • Gan ES  et al. Hypoxia enhances antibody-dependent dengue virus infection. EMBO J 36:1348-1363 (2017). WB . PubMed: 28320741
    • Karagiannis P  et al. IgG4 subclass antibodies impair antitumor immunity in melanoma. J Clin Invest 123:1457-74 (2013). IHC-Fr ; Human . PubMed: 23454746

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    Dear Tech support   Could you please specify what are the methods for which antibody ab46679 was tested for under the FuncS (functional Studies) category? Thanks in advance.

    Read More
    Answer

    Thank you for contacting us. The antibody ab46679 blocks binding of human IgG to FcgammaRIII. The experiment was published in: Tamm A, Schmidt RE: The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding. J Immunol. 1996 Aug 15;157(4):1576-81. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. 

    Read More

    Question

    Thank you for your quick reply. How can I be sure that the Fc portion of the whole IgG antibody molecules do not actually stimulate the Fc receptors that I intend to block?Christine.

    Read More
    Answer

    Thank you for your enquiry. I can confirm the following details which I have obtained from the laboratories: ab41899 CD32 antibody: Unfortunately, we do not have a specific protocol for the use of this product in blocking experiments, this application is supported based upon the literature. There are a number of articles which may be helpful to you, I have listed some examples below: Larsson M, Berge J, Johansson AG, Forsum U. Human dendritic cells handling of binding, uptake and degradation of free and IgG-immune complexed dinitrophenylated human serum albumin in vitro. Immunology. 1997 Jan;90(1):138-46. PMID: 9038724 Bredius RG, Fijen CA, De Haas M, Kuijper EJ, Weening RS, Van de Winkel JG, Out TA. Role of neutrophil Fc gamma RIIa (CD32) and Fc gamma RIIIb (CD16) polymorphic forms in phagocytosis of human IgG1- and IgG3-opsonized bacteria and erythrocytes. Immunology. 1994 Dec;83(4):624-30. PMID: 7875742 Pawar P, Shin PK, Mousa SA, Ross JM, Konstantopoulos K. Fluid shear regulates the kinetics and receptor specificity of Staphylococcus aureus binding to activated platelets. J Immunol. 2004 Jul 15;173(2):1258-65. PMID: 15240718 ab46679 CD16 antibody This antibody can be used for blocking IgG binding to FcγRIII. Further details can be found in the following references: HLDA IV - PART 4: NK13.3 section - NK cell structural studies: Localization of the epitopes of hu CD16 MAbs on the molecular model of CD16, Tamm, A., et al., p. 324-326. Tamm A, Schmidt RE.: The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding. J Immunol. 1996 Aug 15;157(4):1576-81. PMID: 8759741 I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

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