Anti-CD15 抗体 [MMA] (ab17080)
Key features and details
- Mouse monoclonal [MMA] to CD15
- Suitable for: IHC-Fr, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgM
製品の概要
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製品名
Anti-CD15 antibody [MMA]
CD15 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MMA] to CD15 -
由来種
Mouse -
アプリケーション
適用あり: IHC-Fr, Flow Cyt, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-P: Human Hodgkin Lymphoma. Flow Cytometry: Human whole blood IHC-Fr: Human Spleen
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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ポリ/モノ
モノクローナル -
クローン名
MMA -
アイソタイプ
IgM -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17080の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
Use a concentration of 1 µg/ml.
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Flow Cyt |
Use a concentration of 1 µg/ml.
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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IHC-Fr
Use a concentration of 1 µg/ml. |
Flow Cyt
Use a concentration of 1 µg/ml. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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関連性
CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes. -
細胞内局在
Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi. -
別名
- 3 fucosyl N acetyl lactosamine antibody
- 3-FAL antibody
- 3-fucosyl-N-acetyl-lactosamine antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody [MMA] (ab17080)
IHC image of CD15 staining in Human tonsil formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6). The section was incubated with ab17080, 1µg/ml, for 15 mins at room temperature. A Goat polyclonal Secondary Antibody to Mouse IgM secondary antibody (ab97230) was used to detect the primary, and visualized using an HRP conjugated ABC system. The section was counterstained with haematoxylin and mounted with DPX.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre -
Human whole blood stained with ab17080 (right) or mouse IgM isotype control ab91545 (left). Red blood cells of 200ul human whole blood were lysed, then cells were incubated for 30 min on ice in 1x PBS containing 10ug/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab17080 ) or IgM isotype control (ab91545) (100ul at 1ug/ml) for 30 min on ice.
The secondary antibody Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (ab150121) was used at 1/2000 dilution for 30 min at 4° C. Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were collected with the forward and side light-scatter characteristics of viable cells.
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IHC image of CD15 staining in a section of frozen normal human spleen*. The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab17080 at 1µg/ml. The section was then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488), 1/1000)) (shown in green) for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab17080 は 1 報の論文で使用されています。
- Álvarez-Prado ÁF et al. Immunogenomic analysis of human brain metastases reveals diverse immune landscapes across genetically distinct tumors. Cell Rep Med 4:100900 (2023). PubMed: 36652909