Anti-CD15 抗体 [BRA-4F1] (ab115993)
Key features and details
- Mouse monoclonal [BRA-4F1] to CD15
- Suitable for: WB, IHC-P, IHC-Fr, ICC, ICC/IF
- Reacts with: Human
- Isotype: IgM
製品の概要
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製品名
Anti-CD15 antibody [BRA-4F1]
CD15 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [BRA-4F1] to CD15 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, IHC-Fr, ICC, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human CD15. Erythroid myeloid leukemia cells line K562.
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ポジティブ･コントロール
- Reed Sternberg cells in Hodgkin’s lymphoma tissue.
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特記事項
This product was changed from ascites to tissue culture supernatant on 15th June 2017. Lot numbers higher than GR177792-4 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
BRA-4F1 -
アイソタイプ
IgM -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115993の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/100 - 1/200. Predicted molecular weight: 59 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IHC-Fr |
1/50 - 1/100.
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| ICC |
Use at an assay dependent concentration.
(methanol fixed) Use at an assay dependent concentration |
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| ICC/IF |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
1/100 - 1/200. Predicted molecular weight: 59 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
IHC-Fr
1/50 - 1/100. |
|
ICC
Use at an assay dependent concentration. (methanol fixed) Use at an assay dependent concentration |
|
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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関連性
CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes. -
細胞内局在
Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi. -
別名
- 3 fucosyl N acetyl lactosamine antibody
- 3-FAL antibody
- 3-fucosyl-N-acetyl-lactosamine antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab115993 は論文での使用が確認できていません。