Anti-CD147 抗体 [MEM-M6/2] (ab21903)
![Flow Cytometry - Anti-CD147 antibody [MEM-M6/2] (ab21903)](https://www.abcam.co.jp/ps/products/21/ab21903/Images/ab21903-2-Anti-CD147-antibody-MEM-M62-Human-A549-cells-flow-cytometry.jpg "Flow Cytometry - Anti-CD147 antibody [MEM-M6/2] (ab21903)")
Key features and details
- Mouse monoclonal [MEM-M6/2] to CD147
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-CD147 antibody [MEM-M6/2]
CD147 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MEM-M6/2] to CD147 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
A soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the entire extracellular region of CD147 fused to the DNA coding for the hinge region, CH2 and CH3 domain of human IgG1.
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エピトープ
This antibody recognises extracellular Ig domain D1 of CD147 antigen. -
ポジティブ･コントロール
- Flow Cyt: A549 cells.
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特記事項
This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
> 95% (by SDS-PAGE). Purified from TCS -
ポリ/モノ
モノクローナル -
クローン名
MEM-M6/2 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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KO cell lines
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab21903の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use a concentration of 5 µg/ml.
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| 特記事項 |
|---|
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Flow Cyt
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. -
組織特異性
Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 682 Human
- Omim: 109480 Human
- SwissProt: P35613 Human
- Unigene: 501293 Human
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別名
- 5A11 antigen antibody
- 5F7 antibody
- BASI_HUMAN antibody
see all
画像
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Flow Cytometry - Anti-CD147 antibody [MEM-M6/2] (ab21903)
Flow cytometry overlay histogram showing wild-type A549 (green line) and BSG knockout A549 cells (ab273748) stained with ab21903 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab21903) (1x106 in 100μl at 5 μg/ml) for 30 min at 4°C.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min at 4°C.
Isotype control antibody was mouse IgG2bκ (ab170192) used at the same concentration and conditions as the primary antibody (wild-type A549 - black line; BSG knockout A549 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab21903 は論文での使用が確認できていません。