JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB215884

Anti-CD146 抗体 [MCAM/1101]

Anti-CD146 antibody [MCAM/1101]

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Monoclonal CD146 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MCAM.

別名を表示する

CD146, MUC18, MCAM, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

MCAM/1101

アイソタイプ

IgG1

軽鎖のタイプ

kappa

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P

applications

免疫原

Recombinant Full Length Protein corresponding to Human MCAM.

P43121

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Primary incubation for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 8.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.</p>" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
精製に関する特記事項
ab215884 was purified from Bioreactor Concentrate by Protein A/G.
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD146 also known as MCAM or MUC18 is a cell adhesion molecule that belongs to the immunoglobulin superfamily. With a mass of approximately 113 kDa CD146 is expressed mainly on the surface of endothelial cells but it can also be found on smooth muscle cells some T-cells and in certain cancerous tissues. This protein plays a role in cell-cell adhesion contributing to the integrity and signaling functions within tissue microenvironments.
Biological function summary

CD146 influences the migration and organization of cells within the vascular system. It serves as a component of cell adhesion complexes facilitating interactions between endothelial cells and other cell types. Additionally CD146 contributes to angiogenesis the process by which new blood vessels form from existing vasculature which is critical during tissue development and repair. This function makes it an important element in maintaining normal physiological processes.

Pathways

CD146 participates in the Wnt/β-catenin and MAPK signaling pathways which are vital in cell proliferation and differentiation. Through the Wnt/β-catenin pathway CD146 interacts with other proteins like Frizzled receptors to regulate gene transcription. In the MAPK pathway it is linked with signaling cascades that involve proteins such as ERK leading to cellular responses necessary for growth and response to external stimuli.

CD146 has been implicated in melanoma progression and cardiovascular diseases. Its overexpression in melanoma associates it with increased tumor growth and metastasis often involving interactions with other adhesion molecules like integrins. In cardiovascular diseases CD146 relates to atherosclerosis as its expression on endothelial cells can promote inflammatory responses. Understanding these connections provides insights into potential therapeutic targets for treating these conditions.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
See full target information MCAM
style
left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 9:3634 PubMed30194383

2018

Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriel B Ferguson,Ben Van Handel,Maxwell Bay,Petko Fiziev,Tonis Org,Siyoung Lee,Ruzanna Shkhyan,Nicholas W Banks,Mila Scheinberg,Ling Wu,Biagio Saitta,Joseph Elphingstone,A Noelle Larson,Scott M Riester,April D Pyle,Nicholas M Bernthal,Hanna Ka Mikkola,Jason Ernst,Andre J van Wijnen,Michael Bonaguidi,Denis Evseenko
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB216361

PerCP Anti-CD146 antibody [EPR3208]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PerCP Anti-CD146 antibody [EPR3208] (AB216361)

  • 0
  • 0
Primary Antibodies

AB217072

Biotin Anti-CD146 antibody [EPR3208]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-CD146 antibody [EPR3208] (AB217072)

  • 3
  • 1
Primary Antibodies

AB209298

PE Anti-CD146 antibody [EPR3208]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-CD146 antibody [EPR3208] (AB209298)

  • 0
  • 0
Primary Antibodies

AB196448

Alexa Fluor® 488 Anti-CD146 antibody [EPR3208]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD146 antibody [EPR3208] (AB196448)

  • 4
  • 1
Primary Antibodies

AB210072

Anti-CD146 antibody [EPR3208] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-CD146 antibody [EPR3208] - BSA and Azide free (AB210072)

  • 4
  • 1
Primary Antibodies

AB75769

Anti-CD146 antibody [EPR3208]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [EPR3208] (AB75769)

  • 4
  • 41
Primary Antibodies

AB134065

Anti-CD146 antibody [EPR3207(2)]

RabMAb|Recombinant

Western blot - Anti-CD146 antibody [EPR3207(2)] (AB134065)

  • 4
  • 1

組合せ製品

Assay Kits

AB315318

Human CD146 ELISA Kit

SimpleStep

Sandwich ELISA - Human CD146 ELISA Kit (AB315318)

  • 0
  • 0
Primary Antibodies

AB32570

Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal (AB32570)

  • 4
  • 31
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB92574

Anti-CD90 / Thy1 antibody [EPR3132]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3132] (AB92574)

  • 5
  • 6
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com