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Anti-CD14 抗体 [1H5D8] (ab181470)

Submit a review Submit a question References (14)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [1H5D8] (ab181470)
  • Western blot - Anti-CD14 antibody [1H5D8] (ab181470)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD14 antibody [1H5D8] (ab181470)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [1H5D8] (ab181470)
  • Western blot - Anti-CD14 antibody [1H5D8] (ab181470)

Key features and details

  • Mouse monoclonal [1H5D8] to CD14
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-CD14 antibody [1H5D8]
    CD14 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [1H5D8] to CD14

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ICC/IF, IHC-P, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment corresponding to Human CD14 aa 20-214. (Expressed in E.coli).
    Sequence:

    TTPEPCELDDEDFRCVCNFSEPQPDWSEAFQCVSAVEVEIHAGGLNLEPF LKRVDADADPRQYADTVKALRVRRLTVGAAQVPAQLLVGALRVLAYSRLK ELTLEDLKITGTMPPLPLEATGLALSSLRLRNVSWATGRSWLAELQQWLK PGLKVLSIAQAHSPAFSCEQVRAFPALTSLDLSDNPGLGERGLMA


    Database link: P08571
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: Human CD14 (aa 20-214) recombinant protein; CD14 (aa 20-214)-hIgGFc transfected HEK-293 cell lysate. IHC-P: Human cervical cancer and colon tissues. ICC/IF: HepG2 cells.

  • 特記事項

    This product was changed from ascites to supernatant. Lot no’s high than GR281538-10 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab181470の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/200 - 1/1000.
IHC-P
1/200 - 1/1000.
WB
1/500 - 1/2000. Predicted molecular weight: 40 kDa.
特記事項
ICC/IF
1/200 - 1/1000.
IHC-P
1/200 - 1/1000.
WB
1/500 - 1/2000. Predicted molecular weight: 40 kDa.

ターゲット情報

  • 機能

    Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

  • 組織特異性

    Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

  • 配列類似性

    Contains 11 LRR (leucine-rich) repeats.

  • 翻訳後修飾

    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD 14 antibody
      • CD_antigen=CD14 antibody
      • CD14 antibody
      • CD14 antigen antibody
      • CD14 molecule antibody
      • CD14_HUMAN antibody
      • LPS-R antibody
      • Mo2 antibody
      • Monocyte differentiation antigen CD14 antibody
      • Monocyte differentiation antigen CD14 urinary form antibody
      • Monocyte differentiation antigen CD14, membrane-bound form antibody
      • Myeloid cell specific leucine rich glycoprotein antibody
      • Myeloid cell-specific leucine-rich glycoprotein antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [1H5D8] (ab181470)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [1H5D8] (ab181470)

      Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD14 with ab181470 at 1/200 dilution with DAB staining.

    • Western blot - Anti-CD14 antibody [1H5D8] (ab181470)
      Western blot - Anti-CD14 antibody [1H5D8] (ab181470)
      All lanes : Anti-CD14 antibody [1H5D8] (ab181470) at 1/500 dilution

      Lane 1 : Non-transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
      Lane 2 : CD14 (aa 20-214)-hIgGFc transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

      Predicted band size: 40 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-CD14 antibody [1H5D8] (ab181470)
      Immunocytochemistry/ Immunofluorescence - Anti-CD14 antibody [1H5D8] (ab181470)

      Immunofluorescent analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling CD14 with ab181470 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [1H5D8] (ab181470)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [1H5D8] (ab181470)

      Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling CD14 with ab181470 at 1/200 dilution with DAB staining.

    • Western blot - Anti-CD14 antibody [1H5D8] (ab181470)
      Western blot - Anti-CD14 antibody [1H5D8] (ab181470)
      Anti-CD14 antibody [1H5D8] (ab181470) at 1/500 dilution + Human CD14 (aa 20-214) recombinant protein

      Predicted band size: 40 kDa



      Expected MWt is 46.8 kDa.

    参考文献 (14)

    ab181470 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab181470 は 14 報の論文で使用されています。

    • Zhang PP  et al. COVID-19-associated monocytic encephalitis (CAME): histological and proteomic evidence from autopsy. Signal Transduct Target Ther 8:24 (2023). PubMed: 36609561
    • Zhai Z  et al. Attenuation of Rheumatoid Arthritis Through the Inhibition of Tumor Necrosis Factor-Induced Caspase 3/Gasdermin E-Mediated Pyroptosis. Arthritis Rheumatol 74:427-440 (2022). PubMed: 34480835
    • Wang M  et al. Renalase and its receptor, PMCA4b, are expressed in the placenta throughout the human gestation. Sci Rep 12:4953 (2022). PubMed: 35322081
    • Lee HR  et al. CD14+ monocytes and soluble CD14 of synovial fluid are associated with osteoarthritis progression. Arch Rheumatol 37:335-343 (2022). PubMed: 36589618
    • Wu M  et al. FSTL1 promotes growth and metastasis in gastric cancer by activating AKT related pathway and predicts poor survival. Am J Cancer Res 11:712-728 (2021). PubMed: 33791149
    View all Publications for this product

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