AB76000

Anti-CD134 / OX40L receptor 抗体 [EPR17Y]

Anti-CD134 / OX40L receptor antibody [EPR17Y]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal CD134 / OX40L receptor antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 2 publications.

別名を表示する

CD134, TXGP1L, TNFRSF4, Tumor necrosis factor receptor superfamily member 4, ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor

4 Images

Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR17Y] (AB76000)

Flow Cyt

Lab

Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR17Y] (AB76000)

Flow cytometry analysis of Human PBMC treated with 1© : g/ml PHA for 3 days cells labelling CD134 / OX40L receptor with ab76000 at 1/1200 dilution (1.05 μg/ml) (Red). Dylight488 (ab98462) was used as the secondary antibody at 1/2000 dilution.

Unknown

Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (AB76000)

Blocking and diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/1000 dilution

All lanes:

Human tonsil tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 29 kDa

Observed band size: 45 kDa

false

Unknown

Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (AB76000)

Blocking and diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/5000 dilution

Lane 1:

Human lymph node tissue lysate at 10 µg

Lane 2:

Human spleen tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 29 kDa

Observed band size: 45 kDa

false

Unknown

Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (AB76000)

Note : the actual molecular weight is larger than the predicted due to glycoslyation.

All lanes:

Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/500 dilution

Lane 1:

PBMC cell lysates, untreated at 10 µg

Lane 2:

PBMC cell lysates, treated with TPA at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 29 kDa,32 kDa

Observed band size: 45 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR17Y

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.19% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD134 also known as OX40 or TNFRSF4 is a receptor that plays an essential role in immune response regulation. This receptor is part of the tumor necrosis factor receptor (TNFR) superfamily and has a molecular mass of approximately 50 kDa. CD134 is expressed on activated T cells particularly CD4+ T cells and is present on various other immune cells under specific conditions. The receptor binds to the OX40L its ligand which is expressed on antigen-presenting cells such as dendritic cells and B cells.

Biological function summary

CD134 influences T cell proliferation survival and memory formation being an important component of the immune system's adaptive response. OX40L engagement with CD134 enhances the activation and survival of T cells promoting long-term immunity. This interaction does not form part of a larger complex but functions as a direct signaling mechanism that augments T cell receptor (TCR) signaling leading to increased cytokine production and sustained immune responses essential for controlling infections and cancer.

Pathways

CD134 signaling intersects with the nuclear factor-kappa B (NF-κB) and phosphoinositide 3-kinase (PI3K) pathways. These pathways play significant roles in regulating immune responses and cell survival. Through the NF-κB pathway CD134 influences gene transcription related to immune activation. It also connects with other immune regulatory proteins such as CD28 which further potentiates T cell responses and enhances effector functions.

CD134 has connections to autoimmune diseases and cancers. Overactivity of the CD134-OX40L pathway can lead to exacerbated autoimmune conditions such as lupus. This receptor has also been implicated in tumor immunity where its activation enhances the anti-tumor response of T cells. CD134 interacts with CTLA-4 another immune checkpoint protein playing a role in balancing immune activation and regulation within these disease contexts.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity.. (Microbial infection) Acts as a receptor for human herpesvirus 6B/HHV-6B.

See full target information TNFRSF4

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 12:3242-3248 PubMed27882144

2016

Effect of cyclosporin A intervention on the immunological mechanisms of coronary heart disease and restenosis.

Applications

IHC-P

Species

Rabbit

Xuan Wang,Yue-Cheng Hu,Ru-Yan Zhang,Dong-Xia Jin,Yuan Jiang,He-Nan Zhang,Hong-Liang Cong

The Journal of experimental medicine 210:1743-59 PubMed23897980

2013

Inherited human OX40 deficiency underlying classic Kaposi sarcoma of childhood.

Applications

WB, Flow Cyt

Species

Human, Human

Minji Byun,Cindy S Ma,Arzu Akçay,Vincent Pedergnana,Umaimainthan Palendira,Jinjong Myoung,Danielle T Avery,Yifang Liu,Avinash Abhyankar,Lazaro Lorenzo,Monika Schmidt,Hye Kyung Lim,Olivier Cassar,Melanie Migaud,Flore Rozenberg,Nur Canpolat,Gönül Aydogan,Bernhard Fleckenstein,Jacinta Bustamante,Capucine Picard,Antoine Gessain,Emmanuelle Jouanguy,Ethel Cesarman,Martin Olivier,Philippe Gros,Laurent Abel,Michael Croft,Stuart G Tangye,Jean-Laurent Casanova

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD134 / OX40L receptor 抗体 [EPR17Y]

Anti-CD134 / OX40L receptor antibody [EPR17Y]

Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR17Y] (AB76000)

Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (AB76000)

Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (AB76000)

Western blot - Anti-CD134 / OX40L receptor antibody [EPR17Y] (AB76000)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

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Abcam product promise