Anti-CD133 抗体 [mAbcam27699] (ab27699)
Key features and details
- Mouse monoclonal [mAbcam27699] to CD133
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG2b
製品の概要
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製品名
Anti-CD133 antibody [mAbcam27699]
CD133 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [mAbcam27699] to CD133 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Mouse CD133.
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ポジティブ・コントロール
- WB: Mouse kidney tissue lysate.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
mAbcam27699 -
ミエローマ
Sp2 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab27699の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).
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特記事項 |
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WB
Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). |
ターゲット情報
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機能
May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439). -
組織特異性
Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level). -
関連疾患
Retinitis pigmentosa 41
Cone-rod dystrophy 12
Stargardt disease 4
Retinal macular dystrophy 2 -
配列類似性
Belongs to the prominin family. -
翻訳後修飾
Isoform 1 and isoform 2 are glycosylated.
Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis. -
細胞内局在
Apical cell membrane. Cell projection, microvillus membrane. Cell projection, cilium, photoreceptor outer segment. Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine. - Information by UniProt
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参照データベース
- Entrez Gene: 19126 Mouse
- SwissProt: O54990 Mouse
- Unigene: 6250 Mouse
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別名
- AC133 antibody
- Antigen AC133 antibody
- CD133 antibody
see all
画像
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Anti-CD133 antibody [mAbcam27699] (ab27699) at 5 µg + Mouse kidney tissue lysate at 20 µg
Secondary
HRP conjugated goat anti-mouse at 1/5000 dilution
Predicted band size: 97 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa (possible IgG)This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab27699 overnight at 4°C. Antibody binding was detected using a goat anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab27699 は 12 報の論文で使用されています。
- Gisina A et al. Can CD133 Be Regarded as a Prognostic Biomarker in Oncology: Pros and Cons. Int J Mol Sci 24:N/A (2023). PubMed: 38139228
- Matalkah F et al. The mAB 13A4 monoclonal antibody to the mouse PROM1 protein recognizes a structural epitope. PLoS One 17:e0274958 (2022). PubMed: 36215230
- Donnio LM et al. XAB2 dynamics during DNA damage-dependent transcription inhibition. Elife 11:N/A (2022). PubMed: 35880862
- Nosrati A et al. Cancer Stem Cell Markers CD44, CD133 in Primary Gastric Adenocarcinoma. Int J Mol Cell Med 3:279-86 (2014). IHC-P ; Human . PubMed: 25635255
- Lubanska D et al. The cyclin-like protein Spy1 regulates growth and division characteristics of the CD133+ population in human glioma. Cancer Cell 25:64-76 (2014). ICC/IF ; Human . PubMed: 24434210