Anti-CD130 (gp130) 抗体 [B-P4] - BSA and Azide free (ab34325)
![Flow Cytometry - Anti-CD130 (gp130) antibody [B-P4B-P4] - BSA and Azide free (ab34325)](https://www.abcam.co.jp/ps/products/34/ab34325/Images/ab34325-1-38121anticd130gp130antibodybp4flowcytometry.jpg "Flow Cytometry - Anti-CD130 (gp130) antibody [B-P4B-P4] - BSA and Azide free (ab34325)")
Key features and details
- Mouse monoclonal [B-P4] to CD130 (gp130) - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CD130 (gp130) antibody [B-P4] - BSA and Azide free
CD130 (gp130) 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [B-P4] to CD130 (gp130) - BSA and Azide free -
由来種
Mouse -
特異性
Recognises the Gp130, common subunit for IL-6, IL-11, OSM, LIF, CNTF, CT-1 receptors, a 130-140 kDa protein. Inhibits IL-11 activity and binding to gp130, but not CNTF, LIF and OSM activities.
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アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Full length protein corresponding to Human CD130 (gp130). Natural soluble gp130
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特記事項
ab34325 is a carrier free version of ab27357.
This product was changed from ascites to tissue culture supernatant on 4th April 2 19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.3
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Tissue culture supernatant -
特記事項（精製）
This antibody was purified by ion exchange chromatography. It is supplied sterile filtered through 0.22 µm and treated to remove endotoxins. -
ポリ/モノ
モノクローナル -
クローン名
B-P4 -
ミエローマ
x63-Ag8.653 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34325の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | (1) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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Flow Cyt
Use at an assay dependent concentration. |
ターゲット情報
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機能
Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events. -
組織特異性
Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells. -
配列類似性
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
ドメイン
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
翻訳後修飾
Phosphorylation of Ser-782 down-regulates cell surface expression.
Heavily N-glycosylated. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3572 Human
- Omim: 600694 Human
- SwissProt: P40189 Human
- Unigene: 532082 Human
- Unigene: 706627 Human
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別名
- CD130 antibody
- CD130 antigen antibody
- CDw130 antibody
see all
画像
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Flow Cytometry - Anti-CD130 (gp130) antibody [B-P4B-P4] - BSA and Azide free (ab34325)
Ab27357, staining CD130 (gp130) of Eahy 926 cell line by Flow Cytometry.
This image was generated using the ascites version of the product.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab27357).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab34325 は 3 報の論文で使用されています。
- Lee SY et al. IL-6 trans-signaling system in intra-amniotic inflammation, preterm birth, and preterm premature rupture of the membranes. J Immunol 186:3226-36 (2011). WB, IHC-P ; Human . PubMed: 21282511
- Schmitz J et al. SOCS3 exerts its inhibitory function on interleukin-6 signal transduction through the SHP2 recruitment site of gp130. J Biol Chem 275:12848-56 (2000). PubMed: 10777583
- Wijdenes J et al. Interleukin-6 signal transducer gp130 has specific binding sites for different cytokines as determined by antagonistic and agonistic anti-gp130 monoclonal antibodies. Eur J Immunol 25:3474-81 (1995). PubMed: 8566040
