Anti-CD130 (gp130) 抗体 [B-K5] - BSA and Azide free (ab34324)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [B-K5] to CD130 (gp130) - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
製品の概要
-
製品名
Anti-CD130 (gp130) antibody [B-K5] - BSA and Azide free
CD130 (gp130) 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [B-K5] to CD130 (gp130) - BSA and Azide free -
由来種
Mouse -
特異性
Recognises Gp130, common subunit for IL-6, IL-11, OSM, LIF, CNTF, CT-1 receptors, a 130-140 kDa protein.
-
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Full length protein corresponding to Human CD130 (gp130). Natural soluble gp130
Database link: P40189 -
特記事項
This product was changed from ascites to tissue culture supernatant on 4th April 2 19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Constituent: 100% PBS
Sterile-filtered through 0.22 µm. Carrier and preservative free -
キャリア・フリー
はい -
Concentration information loading...
-
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
B-K5 -
ミエローマ
x63-Ag8.653 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34324の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
---|
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
-
機能
Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events. -
組織特異性
Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells. -
配列類似性
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
ドメイン
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
翻訳後修飾
Phosphorylation of Ser-782 down-regulates cell surface expression.
Heavily N-glycosylated. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 3572 Human
- Omim: 600694 Human
- SwissProt: P40189 Human
- Unigene: 532082 Human
- Unigene: 706627 Human
-
別名
- CD130 antibody
- CD130 antigen antibody
- CDw130 antibody
see all
画像
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (2)
ab34324 は 2 報の論文で使用されています。
- Trillet K et al. The glycoprotein GP130 governs the surface presentation of the G protein-coupled receptor APLNR. J Cell Biol 220:N/A (2021). PubMed: 34287648
- Chevalier S et al. Interleukin-6 family of cytokines induced activation of different functional sites expressed by gp130 transducing protein. J Biol Chem 271:14764-72 (1996). PubMed: 8662918