Anti-CD13 抗体 [EPR4059]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [EPR4059] (AB108382)

Immunocytochemistry/ Immunofluorescence analysis of A375 (human malignant melanoma epithelial cell) cells labeling CD13 with purified ab108382 at 1 : 500 (0.7 μg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with None. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (AB108382)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling CD13 with purified ab108382 at 1 : 750 dilution (0.5 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (AB108382)

Unpurified ab108382 showing positive staining in human Prostatic carcinoma tissue.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (AB108382)

Unpurified ab108382 showing positive staining in Normal human tonsil tissue.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (AB108382)

Unpurified ab108382 showing positive staining in human Hepatocellular carcinoma tissue.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (AB108382)

Immunohistochemical staining of paraffin-embedded Human kidney tissue using unpurified ab108382 at a dilution of 1/100.

• IHC-P

AbReview52046****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (AB108382)

Unpurified ab108382 staining CD13 in human kidney tissue sections by Immunohistochemistry (Formaldehyde/PFA-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.05% tween-20 and blocked for 60 minutes at 25°C. Antigen retrieval was by heat mediation. Samples were incubated with primary antibody at a dilution of 1/400 for 1 hour at 25°C. An Alexa Flour^® 488-conjugated donkey anti-rabbit IgG polyclonal (1/1200) was used as the secondary antibody.

This image is courtesy of an anonymous abreview.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (AB108382)

Unpurified ab108382 showing positive staining in human Normal liver tissue.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [EPR4059] (AB108382)

ab108382 staining CD13 in wild-type THP-1 cells (top panel) and ANPEP knockout THP-1 cells (bottom panel) (ab273759). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab108382 at 1/1000 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor^® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor^® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (AB108382)

Unpurified ab108382 showing positive staining in Normal human breast tissue.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (AB108382)

Unpurified ab108382 showing positive staining in human Ovarian carcinoma tissue.

• IP

Lab

Immunoprecipitation - Anti-CD13 antibody [EPR4059] (AB108382)

ab108382 (purified) at 1 : 20 dilution (2ug) immunoprecipitating in THP-1 whole cell lysate. THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10ug
Lane 2 (+) : ab108382 & THP-1 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab108382 in THP-1 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-CD13 antibody [EPR4059] (ab108382)

Predicted band size: 109 kDa

Observed band size: 150 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (AB108382)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling CD13 with Purified ab108382 at 1 : 750 dilution (0.5 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

• WB

Lab

Western blot - Anti-CD13 antibody [EPR4059] (AB108382)

Lanes 1 - 4 : Merged signal (red and green). Green - ab108382 observed at 160 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab108382 was shown to react with CD13 in wild-type THP-1 cells in western blot with loss of signal observed in ANPEP knockout cell line ab273759 (knockout cell lysate ab275505). Wild-type and ANPEP knockout THP-1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab108382 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD13 antibody [EPR4059] (ab108382) at 1/1000 dilution

Lane 1:

Wild-type THP-1 cell lysate at 30 µg

Lane 2:

ANPEP knockout THP-1 cell lysate at 30 µg

Lane 2:

Western blot - Human ANPEP (CD13) knockout THP-1 cell line (<a href='/products/cell-lines/human-anpep-cd13-knockout-thp-1-cell-line-ab273759'>ab273759</a>)

Lane 3:

PANC-1 cell lysate at 30 µg

Lane 4:

HEK-293 cell lysate at 30 µg

Predicted band size: 109 kDa

Observed band size: 160 kDa

false

• WB

Unknown

Western blot - Anti-CD13 antibody [EPR4059] (AB108382)

All lanes:

Western blot - Anti-CD13 antibody [EPR4059] (ab108382) at 1/1000 dilution

Lane 1:

Rat lung lysates at 20 µg

Lane 2:

A375 (Human malignant melanoma epithelial cell) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 109 kDa

Observed band size: 150 kDa

false

• WB

Unknown

Western blot - Anti-CD13 antibody [EPR4059] (AB108382)

All lanes:

Western blot - Anti-CD13 antibody [EPR4059] (ab108382) at 1/1000 dilution

Lane 1:

THP-1 cell lysate at 10 µg

Lane 2:

U937 cell lysate at 10 µg

Lane 3:

A375 cell lysate at 10 µg

Predicted band size: 109 kDa,14 kDa,50 kDa,86 kDa,9 kDa

Observed band size: 150 kDa,50 kDa

false