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リコンビナントRabMAb

Anti-CD11b 抗体 [EPR19387] (ab184308)

Reviews (1) Submit a question References (16)
Western blot - Anti-CD11b antibody [EPR19387] (ab184308)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EPR19387] (ab184308)
  • Immunoprecipitation - Anti-CD11b antibody [EPR19387] (ab184308)
  • Anti-CD11b antibody [EPR19387] (ab184308)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19387] to CD11b
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-CD11b antibody [EPR19387]
    CD11b 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR19387] to CD11b

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IF, IPmore details
    適用なし: Flow Cyt

  • 種交差性

    交差種: Mouse
    非交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: J774A.1, M1 and RAW 264.7 whole cell lysates. ICC/IF: RAW 264.7 cells. Flow Cyt: RAW 264.7 cells. IP: RAW 264.7 whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184308の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 127 kDa).
ICC/IF (1)
1/500.
IP
1/40.
特記事項
WB
1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 127 kDa).
ICC/IF
1/500.
IP
1/40.
追加情報
Is unsuitable for Flow Cyt.

ターゲット情報

  • 機能

    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.

  • 組織特異性

    Predominantly expressed in monocytes and granulocytes.

  • 関連疾患

    Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.

  • 配列類似性

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.

  • ドメイン

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • antigen CD11b (p170) antibody
      • Antigen CD11b p170 antibody
      • CD11 antigen like family member B antibody
      • CD11 antigen-like family member B antibody
      • CD11b antibody
      • CD11b/CD18 antibody
      • CD49d antibody
      • Cell surface glycoprotein MAC-1 subunit alpha antibody
      • Complement component 3 receptor 3 subunit antibody
      • Complement Component Receptor 3 Alpha antibody
      • Complement receptor type 3 antibody
      • Complement receptor type 3, alpha subunit antibody
      • CR 3 alpha chain (CR3A) antibody
      • CR 3 alpha chain antibody
      • CR-3 alpha chain antibody
      • CR3 antibody
      • CR3A antibody
      • F730045J24Rik antibody
      • Integrin Alpha M antibody
      • Integrin alpha M chain antibody
      • Integrin alpha-M antibody
      • Integrin beta 2 alpha subunit antibody
      • Integrin subunit alpha M antibody
      • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
      • ITAM_HUMAN antibody
      • ITGAM antibody
      • Leukocyte adhesion receptor MO1 antibody
      • Ly-40 antibody
      • MAC 1 antibody
      • Mac-1a antibody
      • MAC1 antibody
      • Mac1, alpha subunit antibody
      • MAC1A antibody
      • Macrophage antigen alpha polypeptide antibody
      • MGC117044 antibody
      • Mo1 antibody
      • Mo1, alpha subunit antibody
      • MO1A antibody
      • Neutrophil adherence receptor alpha M subunit antibody
      • Neutrophil adherence receptor antibody
      • SLEB6 antibody
      see all

    画像

    • Western blot - Anti-CD11b antibody [EPR19387] (ab184308)
      Western blot - Anti-CD11b antibody [EPR19387] (ab184308)
      All lanes : Anti-CD11b antibody [EPR19387] (ab184308) at 1/1000 dilution

      Lane 1 : J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate
      Lane 2 : M1 (Mouse myeloblast myeloid leukemia cell line) whole cell lysate
      Lane 3 : WEHI-231 (Mouse B cell lymphoma cell line) whole cell lysate
      Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 127 kDa
      Observed band size: 170 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit (PMID: 10603370). It is exclusively restricted to cells of the myeloid lineages and monocytes, but not in T or B cell lines, so WEHI-231 cell lysate serves as a negative control (PMID: 2457584).

    • Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EPR19387] (ab184308)
      Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EPR19387] (ab184308)

      Immunofluorescent analysis of 100% methanol-fixed RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) and WEHI-231 (Mouse B cell lymphoma cell line) cells labeling CD11b with ab184308 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution (green).

      Confocal image showing membrane staining on RAW 264.7 cells and no staining on WEHI-231 cells (negative cell line).

      The nuclear counter stain is DAPI (blue).

      Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1,000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1,000 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab184308 at 1/500 dilution followed by ab150120  at 1/1,000 dilution.
      -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/1,000 dilution.

    • Immunoprecipitation - Anti-CD11b antibody [EPR19387] (ab184308)
      Immunoprecipitation - Anti-CD11b antibody [EPR19387] (ab184308)

      CD11b was immunoprecipitated from 0.35 mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab184308 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184308 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: RAW 264.7 whole cell lysate, 10µg (Input).

      Lane 2: ab184308 IP in RAW 264.7 whole cell lysate.

      Lane 3: Rabbit IgG,monoclonal [EPR25A]- Isotype Control (ab172730) instead of ab184308 in RAW 264.7 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 8 seconds.

    • Anti-CD11b antibody [EPR19387] (ab184308)
      Anti-CD11b antibody [EPR19387] (ab184308)

    参考文献 (16)

    ab184308 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab184308 は 16 報の論文で使用されています。

    • Lin Y  et al. Caspase 6 promotes innate immune activation by functional crosstalk between RIPK1-IκBα axis in liver inflammation. Cell Commun Signal 21:282 (2023). PubMed: 37828624
    • Qu X  et al. C-X-C Motif Chemokine 3 Promotes the Inflammatory Response of Microglia after Escherichia coli-Induced Meningitis. Int J Mol Sci 24:N/A (2023). PubMed: 37445610
    • Sheng M  et al. Caspase 6/NR4A1/SOX9 signaling axis regulates hepatic inflammation and pyroptosis in ischemia-stressed fatty liver. Cell Death Discov 9:106 (2023). PubMed: 36977670
    • Jiang H  et al. M1 macrophage-derived exosomes and their key molecule lncRNA HOTTIP suppress head and neck squamous cell carcinoma progression by upregulating the TLR5/NF-κB pathway. Cell Death Dis 13:183 (2022). PubMed: 35210436
    • Li Y  et al. Qishen Granule (QSG) Inhibits Monocytes Released From the Spleen and Protect Myocardial Function via the TLR4-MyD88-NF-κB p65 Pathway in Heart Failure Mice. Front Pharmacol 13:850187 (2022). PubMed: 35370707
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD11b antibody [EPR19387]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mice hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Mice hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    投稿 Oct 07 2021

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