Anti-CD11b 抗体 [EPR19373] (ab184307)
![Western blot - Anti-CD11b antibody [EPR19373] (ab184307)](https://www.abcam.co.jp/ps/products/184/ab184307/Images/ab184307-255702-anti-cd11b-antibody-epr19373-western-blot.jpg "Western blot - Anti-CD11b antibody [EPR19373] (ab184307)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19373] to CD11b
- Suitable for: WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
製品の概要
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製品名
Anti-CD11b antibody [EPR19373]
CD11b 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR19373] to CD11b -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt -
種交差性
交差種: Mouse, Rat
非交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Mouse and rat spleen lysates; C6, M1, RAW 264.7 and J774A.1 whole cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR19373 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184307の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 127 kDa).
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| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 127 kDa). |
追加情報
Is unsuitable for Flow Cyt.
ターゲット情報
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機能
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. -
組織特異性
Predominantly expressed in monocytes and granulocytes. -
関連疾患
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
配列類似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
ドメイン
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 16409 Mouse
- Entrez Gene: 25021 Rat
- SwissProt: P05555 Mouse
- Unigene: 262106 Mouse
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別名
- antigen CD11b (p170) antibody
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
see all
画像
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Western blot - Anti-CD11b antibody [EPR19373] (ab184307)Lanes 1-3 : Anti-CD11b antibody [EPR19373] (ab184307) at 1/1000 dilution
Lanes 4-5 : Anti-CD11b antibody [EPR19373] (ab184307) at 1/5000 dilution
Lane 1 : Mouse spleen lysate
Lane 2 : M1 (Mouse myeloblast myeloid leukaemia cell line) whole cell lysate
Lane 3 : WEHI-231 (Mouse lymphoblast B cell lymphoma cell line) whole cell lysate
Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 5 : J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 127 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit (PMID: 10603370). It is exclusively restricted to cells of the myeloid lineages and monocytes, but not in T or B cell lines, so WEHI-231 cell lysate serves as a negative control (PMID: 2457584).
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![Western blot - Anti-CD11b antibody [EPR19373] (ab184307)](https://www.abcam.co.jp/ps/products/184/ab184307/Images/ab184307-255701-anti-cd11b-antibody-epr19373-western-blot.jpg)
Western blot - Anti-CD11b antibody [EPR19373] (ab184307)All lanes : Anti-CD11b antibody [EPR19373] (ab184307) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 127 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit (PMID: 10603370).
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![Anti-CD11b antibody [EPR19373] (ab184307)](https://www.abcam.co.jp/ps/products/184/ab184307/Images/ab184307-5-benefits-of-recombinant-antibodies.png)
Anti-CD11b antibody [EPR19373] (ab184307)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (2)
ab184307 は 2 報の論文で使用されています。
- Zhang A et al. Biochemically functionalized probes for cell-type-specific targeting and recording in the brain. Sci Adv 9:eadk1050 (2023). PubMed: 38019917
- Wang C et al. Hippocampal microRNA-26a-3p deficit contributes to neuroinflammation and behavioral disorders via p38 MAPK signaling pathway in rats. J Neuroinflammation 19:283 (2022). PubMed: 36434679