Anti-CCR8 抗体 [E77] (ab32399)
![Western blot - Anti-CCR8 antibody [E77] (ab32399)](https://www.abcam.co.jp/ps/products/32/ab32399/Images/ab32399-383292-anti-ccr8-antibody-e77-westernblot-a431-human.jpg "Western blot - Anti-CCR8 antibody [E77] (ab32399)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E77] to CCR8
- Suitable for: ELISA, WB, IP
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
製品の概要
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製品名
Anti-CCR8 antibody [E77]
CCR8 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [E77] to CCR8 -
由来種
Rabbit -
特異性
Does not cross-react with other G-protein coupled receptor 1 family members. -
アプリケーション
適用あり: ELISA, WB, IPmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Synthetic peptide corresponding to Human CCR8.
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エピトープ
Residues in loop 2 extracellular domain of human CCR8 -
ポジティブ･コントロール
- WB: Human Thymus and spleen tissue lysates; A431 cell lysate IP: Human thymus tissue lysate
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
E77 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32399の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| WB |
1/1000 - 1/2000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
|
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| IP |
1/110.
|
| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
WB
1/1000 - 1/2000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
|
IP
1/110. |
ターゲット情報
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機能
Receptor for the chemokine CCL1/SCYA1/I-309. May regulate monocyte chemotaxis and thymic cell line apoptosis. Alternative coreceptor with CD4 for HIV-1 infection. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1237 Human
- Omim: 601834 Human
- SwissProt: P51685 Human
- Unigene: 113222 Human
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別名
- C C chemokine receptor type 8 antibody
- C C CKR 8 antibody
- C C motif chemokine receptor 8 antibody
see all
画像
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Western blot - Anti-CCR8 antibody [E77] (ab32399)Anti-CCR8 antibody [E77] (ab32399) at 1/1000 dilution (Purified) + A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDaBlocking/Diluting Buffer and concentration: 5% NFDM/TBST
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![Immunoprecipitation - Anti-CCR8 antibody [E77] (ab32399)](https://www.abcam.co.jp/ps/products/32/ab32399/Images/ab32399-330911-anti-ccr8-antibody-e77-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CCR8 antibody [E77] (ab32399)ab32399 (purified) at 1/110 dilution (20 µg/mL) immunoprecipitating CCR8 in Human thymus lysate. Human thymus lysate 10 µgab32399 & Human thymus lysateRabbit monoclonal IgG (ab172730) instead of ab32399 in Human thymus lysate
For western blotting, ab32399 at 1/500 dilution (4.45 µg/mL) and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST . -
![ELISA - Anti-CCR8 antibody [E77] (ab32399)](https://www.abcam.co.jp/ps/products/32/ab32399/Images/ab32399-370015-anti-ccr8-antibody-e77-elisa.jpg)
ELISA - Anti-CCR8 antibody [E77] (ab32399)ELISA using Human CCR8 Antigen at a concentration of 1000 ng/ml; and purified ab32399 at 1000-0 ng/ml. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at a 1:2500 dilution.
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![Western blot - Anti-CCR8 antibody [E77] (ab32399)](https://www.abcam.co.jp/ps/products/32/ab32399/Images/ab32399-330961-anti-ccr8-antibody-e77-western-blot.jpg)
Western blot - Anti-CCR8 antibody [E77] (ab32399)Anti-CCR8 antibody [E77] (ab32399) at 1/5000 dilution (unpurified) + Human Thymus lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa -
![Western blot - Anti-CCR8 antibody [E77] (ab32399)](https://www.abcam.co.jp/ps/products/32/ab32399/Images/ab32399-330960-anti-ccr8-antibody-e77-western-blot.jpg)
Western blot - Anti-CCR8 antibody [E77] (ab32399)Anti-CCR8 antibody [E77] (ab32399) at 1/5000 dilution (unpurified) + Human spleen tissue lysate at 15 µg
Secondary
Goat anti-Rabbit IgG, (H+L), peroxidase conjugated at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa -
![Anti-CCR8 antibody [E77] (ab32399)](https://www.abcam.co.jp/ps/products/32/ab32399/Images/ab32399-10-benefits-of-recombinant-antibodies.png)
Anti-CCR8 antibody [E77] (ab32399)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab32399 は 1 報の論文で使用されています。
- Liu Y & Zhang Y Hsa_circ_0134111 promotes osteoarthritis progression by regulating miR-224-5p/CCL1 interaction. Aging (Albany NY) 13:20383-20394 (2021). PubMed: 34413269