Anti-CCR8 抗体 [E77] (ab32399)
![Western blot - Anti-CCR8 antibody [E77] (ab32399)](https://www.abcam.co.jp/ps/products/32/ab32399/Images/ab32399-383292-anti-ccr8-antibody-e77-westernblot-a431-human.jpg "Western blot - Anti-CCR8 antibody [E77] (ab32399)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E77] to CCR8
- Suitable for: ELISA, WB, IP
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
製品の概要
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製品名
Anti-CCR8 antibody [E77]
CCR8 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [E77] to CCR8 -
由来種
Rabbit -
特異性
Does not cross-react with other G-protein coupled receptor 1 family members. -
アプリケーション
適用あり: ELISA, WB, IPmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Synthetic peptide corresponding to Human CCR8.
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エピトープ
Residues in loop 2 extracellular domain of human CCR8 -
ポジティブ･コントロール
- WB: Human Thymus and spleen tissue lysates; A431 cell lysate IP: Human thymus tissue lysate
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
E77 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32399の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
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| WB |
1/1000 - 1/2000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
|
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| IP |
1/110.
|
| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
WB
1/1000 - 1/2000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
|
IP
1/110. |
ターゲット情報
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機能
Receptor for the chemokine CCL1/SCYA1/I-309. May regulate monocyte chemotaxis and thymic cell line apoptosis. Alternative coreceptor with CD4 for HIV-1 infection. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1237 Human
- Omim: 601834 Human
- SwissProt: P51685 Human
- Unigene: 113222 Human
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別名
- C C chemokine receptor type 8 antibody
- C C CKR 8 antibody
- C C motif chemokine receptor 8 antibody
see all
画像
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Western blot - Anti-CCR8 antibody [E77] (ab32399)Anti-CCR8 antibody [E77] (ab32399) at 1/1000 dilution (Purified) + A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDaBlocking/Diluting Buffer and concentration: 5% NFDM/TBST
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![Immunoprecipitation - Anti-CCR8 antibody [E77] (ab32399)](https://www.abcam.co.jp/ps/products/32/ab32399/Images/ab32399-330911-anti-ccr8-antibody-e77-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CCR8 antibody [E77] (ab32399)ab32399 (purified) at 1/110 dilution (20 µg/mL) immunoprecipitating CCR8 in Human thymus lysate. Human thymus lysate 10 µgab32399 & Human thymus lysateRabbit monoclonal IgG (ab172730) instead of ab32399 in Human thymus lysate
For western blotting, ab32399 at 1/500 dilution (4.45 µg/mL) and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST . -
![ELISA - Anti-CCR8 antibody [E77] (ab32399)](https://www.abcam.co.jp/ps/products/32/ab32399/Images/ab32399-370015-anti-ccr8-antibody-e77-elisa.jpg)
ELISA - Anti-CCR8 antibody [E77] (ab32399)ELISA using Human CCR8 Antigen at a concentration of 1000 ng/ml; and purified ab32399 at 1000-0 ng/ml. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at a 1:2500 dilution.
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![Western blot - Anti-CCR8 antibody [E77] (ab32399)](https://www.abcam.co.jp/ps/products/32/ab32399/Images/ab32399-330961-anti-ccr8-antibody-e77-western-blot.jpg)
Western blot - Anti-CCR8 antibody [E77] (ab32399)Anti-CCR8 antibody [E77] (ab32399) at 1/5000 dilution (unpurified) + Human Thymus lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa -
![Western blot - Anti-CCR8 antibody [E77] (ab32399)](https://www.abcam.co.jp/ps/products/32/ab32399/Images/ab32399-330960-anti-ccr8-antibody-e77-western-blot.jpg)
Western blot - Anti-CCR8 antibody [E77] (ab32399)Anti-CCR8 antibody [E77] (ab32399) at 1/5000 dilution (unpurified) + Human spleen tissue lysate at 15 µg
Secondary
Goat anti-Rabbit IgG, (H+L), peroxidase conjugated at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa -
![Anti-CCR8 antibody [E77] (ab32399)](https://www.abcam.co.jp/ps/products/32/ab32399/Images/ab32399-10-benefits-of-recombinant-antibodies.png)
Anti-CCR8 antibody [E77] (ab32399)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab32399 は 2 報の論文で使用されています。
- Liu Y & Zhang Y Hsa_circ_0134111 promotes osteoarthritis progression by regulating miR-224-5p/CCL1 interaction. Aging (Albany NY) 13:20383-20394 (2021). PubMed: 34413269
- Fujikawa M et al. Chemokine (C-C Motif) Ligand 1 Derived from Tumor-Associated Macrophages Contributes to Esophageal Squamous Cell Carcinoma Progression via CCR8-Mediated Akt/Proline-Rich Akt Substrate of 40 kDa/Mammalian Target of Rapamycin Pathway. Am J Pathol 191:686-703 (2021). PubMed: 33460563